Carbohydrate Binding Module Family 80 - Revision history

Harry Brumer: Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

2021-12-18T21:17:04Z

Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

Elizabeth Ficko-Blean at 09:41, 24 September 2018

2018-09-24T09:41:15Z

Elizabeth Ficko-Blean at 09:40, 24 September 2018

2018-09-24T09:40:53Z

Elizabeth Ficko-Blean at 11:52, 29 August 2018

2018-08-29T11:52:29Z

Elizabeth Ficko-Blean at 09:29, 27 August 2018

2018-08-27T09:29:15Z

Elizabeth Ficko-Blean at 09:28, 27 August 2018

2018-08-27T09:28:28Z

Elizabeth Ficko-Blean at 09:24, 27 August 2018

2018-08-27T09:24:52Z

Elizabeth Ficko-Blean at 09:12, 27 August 2018

2018-08-27T09:12:27Z

Immacolata Venditto at 13:13, 23 August 2018

2018-08-23T13:13:39Z

Immacolata Venditto at 13:11, 23 August 2018

2018-08-23T13:11:46Z

@@ Line 1: / Line 1: @@
 <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption -->
 {{CuratorApproved}}
-* [[Author]]: ^^^Immacolata Venditto^^^
+* [[Author]]: [[User:Immacolata Venditto|Immacolata Venditto]]
-* [[Responsible Curator]]:  ^^^Harry Gilbert^^^
+* [[Responsible Curator]]:  [[User:Harry Gilbert|Harry Gilbert]]
 ----

@@ Line 1: / Line 1: @@
 <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption -->
-{{UnderConstruction}}
+{{CuratorApproved}}
 * [[Author]]: ^^^Immacolata Venditto^^^
 * [[Responsible Curator]]:  ^^^Harry Gilbert^^^

@@ Line 24: / Line 24: @@
 == Functionalities ==
-CBM80 modules play an enzyme-targeting role in a highly complex scaffold that is specific to the ''Ruminococcus'' <cite>Bensoussan2017</cite>. CBM80<sub>RfGH5-1/2</sub>, a module in an enzyme that contains [[GH5]]_7 ([http://www.cazy.org/GH5_7.html CAZy - GH5_7]) and [[GH5]]_4 catalytic modules ([http://www.cazy.org/GH5_4.html CAZy - GH5_4]), binds β-glucans and  β-mannans <cite>VendittoI2016</cite>. Other examples of CBMs that recognize both β-1,4-glucans and β-1,4-mannans are found in families [[CBM16]] <cite>BaeB2008</cite> and [[CBM29]] <cite>CharnockSJ2002</cite>. CBM80<sub>RfGH5</sub>, a module in an enzyme that contains a [[GH5]]_4 ([http://www.cazy.org/GH5_4.html CAZy - GH5_4]) catalytic module, displays specificity for β-glucans <cite>VendittoI2016</cite>.
+''R. flavefaciens'' forms a multi-enzyme cellulosome complex that plays an integral role in the ability of this bacterium to degrade plant cell wall polysaccharides <cite>BergMiller2009</cite>. CBM80 modules play an enzyme-targeting role in a highly complex scaffold that is specific to the ''Ruminococcus'' <cite>Bensoussan2017</cite>.  CBM80<sub>RfGH5-1/2</sub>, a module in an enzyme that contains [[GH5]]_7 ([http://www.cazy.org/GH5_7.html CAZy - GH5_7]) and [[GH5]]_4 catalytic modules ([http://www.cazy.org/GH5_4.html CAZy - GH5_4]), binds β-glucans and  β-mannans <cite>VendittoI2016</cite>. Other examples of CBMs that recognize both β-1,4-glucans and β-1,4-mannans are found in families [[CBM16]] <cite>BaeB2008</cite> and [[CBM29]] <cite>CharnockSJ2002</cite>. CBM80<sub>RfGH5</sub>, a module in an enzyme that contains a [[GH5]]_4 ([http://www.cazy.org/GH5_4.html CAZy - GH5_4]) catalytic module, displays specificity for β-glucans <cite>VendittoI2016</cite>.
 == Family Firsts ==
@@ Line 37: / Line 37: @@
 #BaeB2008 pmid=18025086
 #CharnockSJ2002 pmid=12391332
 </biblio>
 [[Category:Carbohydrate Binding Module Families|CBM080]]

@@ Line 24: / Line 24: @@
 == Functionalities ==
-CBM80 modules play an enzyme-targeting role that is specific to the ''Ruminococcus'' in a highly complex scaffold <cite>Bensoussan2017</cite>. CBM80<sub>RfGH5-1/2</sub>, a module in an enzyme that contains [[GH5]]_7 ([http://www.cazy.org/GH5_7.html CAZy - GH5_7]) and [[GH5]]_4 catalytic modules ([http://www.cazy.org/GH5_4.html CAZy - GH5_4]), binds β-glucans and  β-mannans <cite>VendittoI2016</cite>. Other examples of CBMs that recognize both β-1,4-glucans and β-1,4-mannans are found in families [[CBM16]] <cite>BaeB2008</cite> and [[CBM29]] <cite>CharnockSJ2002</cite>. CBM80<sub>RfGH5</sub>, a module in an enzyme that contains a [[GH5]]_4 ([http://www.cazy.org/GH5_4.html CAZy - GH5_4]) catalytic module, displays specificity for β-glucans <cite>VendittoI2016</cite>.
+CBM80 modules play an enzyme-targeting role in a highly complex scaffold that is specific to the ''Ruminococcus'' <cite>Bensoussan2017</cite>. CBM80<sub>RfGH5-1/2</sub>, a module in an enzyme that contains [[GH5]]_7 ([http://www.cazy.org/GH5_7.html CAZy - GH5_7]) and [[GH5]]_4 catalytic modules ([http://www.cazy.org/GH5_4.html CAZy - GH5_4]), binds β-glucans and  β-mannans <cite>VendittoI2016</cite>. Other examples of CBMs that recognize both β-1,4-glucans and β-1,4-mannans are found in families [[CBM16]] <cite>BaeB2008</cite> and [[CBM29]] <cite>CharnockSJ2002</cite>. CBM80<sub>RfGH5</sub>, a module in an enzyme that contains a [[GH5]]_4 ([http://www.cazy.org/GH5_4.html CAZy - GH5_4]) catalytic module, displays specificity for β-glucans <cite>VendittoI2016</cite>.
 == Family Firsts ==

@@ Line 18: / Line 18: @@
 == Ligand specificities ==
 CBM80 is a small bacterial CBM family comprising around 96 amino acids and identified in the ''Ruminococcus flavefaciens'' cellulosome <cite>RinconMT2010</cite>. The only characterized CBM80 modules are CBM80<sub>RfGH5-1/2</sub> and CBM80<sub>RfGH5</sub>, which are components of enzymes containing catalytic modules derived from [[GH5]]_4 ([http://www.cazy.org/GH5_4.html CAZy - GH5_4]) and [[GH5]]_7 ([http://www.cazy.org/GH5_7.html CAZy - GH5_7]) for CBM80<sub>RfGH5-1/2</sub> and [[GH5]]_4 ([http://www.cazy.org/GH5_4.html CAZy - GH5_4]) for CBM80<sub>RfGH5</sub> <cite>VendittoI2016</cite>.
-CBM80<sub>RfGH5-1/2</sub> and CBM80<sub>RfGH5</sub> display specificity for β-1,4- and mixed linked β-1,3-1,4-glucans, while CBM80<sub>RfGH5-1/2</sub> also binds β-1,4-mannans <cite>VendittoI2016</cite>. CBM80<sub>RfGH5-1/2</sub> binds galactomannan in addition to the β-glucans with affinities in the range of 10<sup>4</sup> M<sup>-1</sup> to 10<sup>5</sup> M<sup>-1</sup> <cite>VendittoI2016</cite>. The endo-mode of binding to soluble polysaccharide indicates that CBM80 is a [[Carbohydrate-binding_modules#Types|type B]] CBM <cite>VendittoI2016</cite>. The key residues implicated in ligand binding were identified by site-direct mutagenesis (Figure 1) <cite>VendittoI2016</cite>. Alanine substitution of Trp453 and Trp489 revealed a complete abrogation of binding to β-glucans and β-mannans, showing the importance of tryptophan residues in ligand recognition <cite>VendittoI2016</cite>. CBMs that bind to β-1,4-glycans typically contain three aromatic residues that make apolar interactions with sugars <cite>CharnockSJ2002</cite>. The predicted polar interactions between the protein and β-glycans have very little influence on affinity <cite>VendittoI2016</cite>.
+CBM80<sub>RfGH5-1/2</sub> and CBM80<sub>RfGH5</sub> display specificity for β-1,4- and mixed linked β-1,3-1,4-glucans, while CBM80<sub>RfGH5-1/2</sub> also binds β-1,4-mannans <cite>VendittoI2016</cite>. CBM80<sub>RfGH5-1/2</sub> binds galactomannan in addition to the β-glucans with affinities in the range of 10<sup>4</sup> M<sup>-1</sup> to 10<sup>5</sup> M<sup>-1</sup> <cite>VendittoI2016</cite>. The endo-mode of binding to soluble polysaccharide indicates that CBM80 is a [[Carbohydrate-binding_modules#Types|type B]] CBM <cite>VendittoI2016</cite>. The key residues implicated in CBM80<sub>RfGH5-1/2</sub> ligand binding were identified by site-direct mutagenesis (Figure 1) <cite>VendittoI2016</cite>. Alanine substitution of Trp453 and Trp489 revealed a complete abrogation of binding to β-glucans and β-mannans, showing the importance of tryptophan residues in ligand recognition <cite>VendittoI2016</cite>. CBMs that bind to β-1,4-glycans typically contain three aromatic residues that make apolar interactions with sugars <cite>CharnockSJ2002</cite>. The predicted polar interactions between the protein and β-glycans have very little influence on affinity <cite>VendittoI2016</cite>.
 == Structural Features ==
 [[File:CBM80.jpg|thumb|300px|right|'''Figure 1.'''  Crystal structure of CBM80<sub>RfGH5-1/2</sub>. The residues that contribute to ligand recognition are shown.]]

@@ Line 25: / Line 25: @@
 == Functionalities ==
-CBM80 modules play an enzyme-targeting role that is specific to the Ruminococcusin a highly complex scaffold <cite>Bensoussan2017</cite>. CBM80<sub>RfGH5-1/2</sub>, a module in an enzyme that contains GH5_7 ([http://www.cazy.org/GH5_7.html CAZy - GH5_7]) and GH5_4 catalytic modules ([http://www.cazy.org/GH5_4.html CAZy - GH5_4]), binds β-glucans and  β-mannans <cite>VendittoI2016</cite>. Other examples of CBMs that recognize both β-1,4-glucans and β-1,4-mannans are found in families [[CBM16]] <cite>BaeB2008</cite> and [[CBM29]] <cite>CharnockSJ2002</cite>. The key residues implicated in ligand binding were identified by site-direct mutagenesis. Alanine substitution of Trp453 and Trp489 revealed a complete abrogation of binding to β-glucans and β-mannans, showing the importance of tryptophan residues in ligand recognition <cite>VendittoI2016</cite>. CBMs that bind to β-1,4-glycans typically contain three aromatic residues that make apolar interactions with sugars <cite>CharnockSJ2002</cite>. The predicted polar interactions between the protein and β-glycans have very little influence on affinity <cite>VendittoI2016</cite>.
+CBM80 modules play an enzyme-targeting role that is specific to the ''Ruminococcus'' in a highly complex scaffold <cite>Bensoussan2017</cite>. CBM80<sub>RfGH5-1/2</sub>, a module in an enzyme that contains GH5_7 ([http://www.cazy.org/GH5_7.html CAZy - GH5_7]) and GH5_4 catalytic modules ([http://www.cazy.org/GH5_4.html CAZy - GH5_4]), binds β-glucans and  β-mannans <cite>VendittoI2016</cite>. Other examples of CBMs that recognize both β-1,4-glucans and β-1,4-mannans are found in families [[CBM16]] <cite>BaeB2008</cite> and [[CBM29]] <cite>CharnockSJ2002</cite>. The key residues implicated in ligand binding were identified by site-direct mutagenesis. Alanine substitution of Trp453 and Trp489 revealed a complete abrogation of binding to β-glucans and β-mannans, showing the importance of tryptophan residues in ligand recognition <cite>VendittoI2016</cite>. CBMs that bind to β-1,4-glycans typically contain three aromatic residues that make apolar interactions with sugars <cite>CharnockSJ2002</cite>. The predicted polar interactions between the protein and β-glycans have very little influence on affinity <cite>VendittoI2016</cite>.
 == Family Firsts ==
@@ Line 35: / Line 35: @@
 #RinconMT2010 pmid=20814577
 #VendittoI2016 pmid=27298375
 #BaeB2008 pmid=18025086
 #CharnockSJ2002 pmid=12391332