Polysaccharide Lyases - Revision history

Harry Brumer: Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

2021-12-18T21:17:27Z

Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

Harry Brumer: Added some references.

2014-10-10T17:52:17Z

Added some references.

Michael Suits at 17:23, 9 October 2014

2014-10-09T17:23:41Z

Spencer Williams at 00:44, 29 November 2013

2013-11-29T00:44:32Z

Harry Brumer: Created page with " {{UnderConstruct..."

2013-09-20T18:03:38Z

Created page with " {{UnderConstruct..."

New page

{{UnderConstruction}}
* Author: ^^^Michael Suits^^^ and ^^^Wade Abbott^^^
* Responsible Curator: ^^^Spencer Williams^^^
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== Introduction ==
The Polysaccharide Lyases (PLs) have been grouped into families and subfamilies within the Carbohydrate-Active Enzyme (CAZy) framework <cite>Lombard2010</cite>. Please see the following reviews on PL classification, specificities, and structures <cite>Lombard2010 Garron2010</cite>

== References ==
<biblio>
#Lombard2010 pmid=20925655
#Garron2010 pmid=20805221
</biblio>

[[Category:Definitions and explanations]]

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 == Introduction ==
-Polysaccharide lyases (PLs) cleave uronic acid-containing polysaccharides via a β-elimination mechanism to generate an unsaturated hexenuronic acid residue and a new reducing end at the point of cleavage. PLs have been grouped into families and subfamilies within the Carbohydrate-Active Enzyme (CAZy) framework <cite>Lombard2010</cite>.  Please see the following reviews on PL classification, specificities, and structures <cite>Lombard2010 Garron2010</cite>
+Polysaccharide lyases (PLs) cleave uronic acid-containing polysaccharides via a β-elimination mechanism to generate an unsaturated hexenuronic acid residue and a new reducing end at the point of cleavage <cite>Lombard2010 Garron2010</cite>. PLs have been grouped into families and subfamilies within the Carbohydrate-Active Enzyme (CAZy) framework and that the PLs included are restricted to those operating via ''syn''- and ''anti''-elimination mechanisms <cite>Lombard2010</cite>. In this way, they are distinct from carbon-oxygen lyases that modify polysaccharide composition. Broadly, the mechanism of PL action can be described as consisting of three events: (i) abstraction of the C-5 proton on the sugar ring of a uronic acid or ester by a charge stabilizing cation such as Ca<sup>2+</sup> or a basic amino acid side chain, (ii) stabilization of the resulting anion by charge delocalization onto the C-6 carbonyl group, and (iii) lytic cleavage of the O-4:C-4 bonding that is facilitated by proton donation from a catalytic acid <cite>Lombard2010</cite>.  Please see the following reviews on PL classification, specificities, and structures <cite>Lombard2010 Garron2010</cite>.
 == References ==

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 {{UnderConstruction}}
-* Author: ^^^Michael Suits^^^ and ^^^Wade Abbott^^^
+* Author: [[User:Michael Suits|Michael Suits]] and [[User:Wade Abbott|Wade Abbott]]
-* Responsible Curator:  ^^^Spencer Williams^^^
+* Responsible Curator:  [[User:Spencer Williams|Spencer Williams]]
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 == Introduction ==
-Polysaccharide lyases (PLs) cleave uronic acid-containing polysaccharides via a β-elimination mechanism to generate an unsaturated hexenuronic acid residue and a new reducing end at the point of cleavage <cite>Lombard2010 Garron2010</cite>. PLs have been grouped into families and subfamilies within the Carbohydrate-Active Enzyme (CAZy) framework and that the PLs included are restricted to those operating via ''syn''- and ''anti''-elimination mechanisms <cite>Lombard2010</cite>. In this way, they are distinct from carbon-oxygen lyases that modify polysaccharide composition. Broadly, the mechanism of PL action can be described as consisting of three events: (i) abstraction of the C-5 proton on the sugar ring of a uronic acid or ester by a charge stabilizing cation such as Ca<sup>2+</sup> or a basic amino acid side chain, (ii) stabilization of the resulting anion by charge delocalization onto the C-6 carbonyl group, and (iii) lytic cleavage of the O-4:C-4 bonding that is facilitated by proton donation from a catalytic acid <cite>Lombard2010</cite>.  Please see the following reviews on PL classification, specificities, and structures <cite>Lombard2010 Garron2010</cite>.
+Polysaccharide lyases (PLs) cleave uronic acid-containing polysaccharides via a β-elimination mechanism to generate an unsaturated hexenuronic acid residue and a new reducing end at the point of cleavage <cite>Lombard2010 Garron2010</cite>. PLs have been grouped into families and subfamilies within the Carbohydrate-Active Enzyme (CAZy) framework and that the PLs included are restricted to those operating via ''syn''- and ''anti''-elimination mechanisms <cite>Lombard2010</cite>. In this way, they are distinct from carbon-oxygen lyases that modify polysaccharide composition. Broadly, the mechanism of PL action can be described as consisting of three events: (i) abstraction of the C-5 proton on the sugar ring of a uronic acid or ester by a charge stabilizing cation such as Ca<sup>2+</sup> or a basic amino acid side chain, (ii) stabilization of the resulting anion by charge delocalization onto the C-6 carbonyl group, and (iii) lytic cleavage of the O-4:C-4 bonding that is facilitated by proton donation from a catalytic acid <cite>Lombard2010 YipWithers2006</cite>.  Please see the following reviews on PL classification, specificities, mechanisms, and structures <cite>Lombard2010 Garron2010 Garron2014 YipWithers2006</cite>.
 == References ==
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 #Lombard2010 pmid=20925655
 #Garron2010 pmid=20805221
 </biblio>
 [[Category:Definitions and explanations]]