User:Hiroyuki Nakai - Revision history

Harry Brumer: added to contributor category

2011-05-26T06:33:39Z

added to contributor category

Hiroyuki Nakai at 02:19, 26 May 2011

2011-05-26T02:19:07Z

Hiroyuki Nakai: Created page with "'''Hiroyuki Nakai''' is an assistant professor at Food Glycoscience Laboratory in Graduate School of Science and Technology, Niigata University. He obtained Ph. D from Division o..."

2011-05-25T07:25:00Z

Created page with "'''Hiroyuki Nakai''' is an assistant professor at Food Glycoscience Laboratory in Graduate School of Science and Technology, Niigata University. He obtained Ph. D from Division o..."

New page

'''Hiroyuki Nakai''' is an assistant professor at Food Glycoscience Laboratory in Graduate School of Science and Technology, Niigata University. He obtained Ph. D from Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University in 2005, under the supervision of Prof. Atsuo Kimura and Prof. Seiya Chiba. He joined the group of Prof. Birte Svensson at Enzyme and Protein Chemistry, Department of Systems Biology, the Technical University of Denmark as post-doctoral fellow (2007) and assistant professor (2008-2010), working on chemoenzymatic synthesis of oligosaccharides by glycoside hydrolase and phosphorylase catalysing transglycosylation and reverse phosphorolysis, respectively. His recent research interests concern structure and function of Carbohydrate Active enZymes, enzymatic synthesis of oligosaccharides showing healthful effect on human intestine, antibacterial activity, and immunostimulatory activity.

* Nakai H., Abou Hachem M., Petersen B.O., Westphal Y., Mannerstedt K., Baumann M.J., Dilokpimol A., Schols H.A., Duus J.Ø., Svensson B. Efficient chemoenzymatic oligosaccharide synthesis by reverse phosphorolysis using cellobiose phosphorylase and cellodextrin phosphorylase from ''Clostridium thermocellum.''　Biochimie (2010) 92, 1818–1826.

* Nakai H., Baumann M.J., Petersen B.O., Westphal Y., Abou Hachem M., Dilokpimol A., Duus J.Ø., Schols H.A., Svensson B.　''Aspergillus nidulans'' α-galactosidase of glycoside hydrolase family 36 catalyses the formation of α-galacto-oligosaccharides by transglycosylation. The FEBS Journal (2010) 277, 3538–3551.

* Nakai H., Petersen B.O., Westphal Y., Dilokpimol A., Abou Hachem M., Duus J.Ø., Schols H.A., Svensson B. Rational engineering of ''Lactobacillus acidophilus'' NCFM maltose phosphorylase into either trehalose or kojibiose dual specificity phosphorylase. Protein Engineering Design & Selection (2010) 23, 781–787.

* Nakai H., Dilokpimol A., Abou Hachem M., Svensson B. Efficient one-pot enzymatic synthesis of α-(1→4)-glucosidic disaccharides through a coupled reaction catalysed by ''Lactobacillus acidophilus'' NCFM maltose phosphorylase. Carbohydrate Research (2010) 345, 1061–1064.

* Nakai H., Baumann M.J., Petersen B.O., Westphal Y., Schols H.A., Dilokpimol A., Abou Hachem M., Lahtinen S.J., Duus J.Ø., Svensson B. The maltodextrin transport system and metabolism in ''Lactobacillus acidophilus'' NCFM and production of novel α-glucosides through reverse phosphorolysis by maltose phosphorylase. The FEBS Journal (2009) 276, 7353–7365.

@@ Line 1: / Line 1: @@
-[[Image:Nakai.jpg|thumb|200px|right]]
+[[Image:Nakai.jpg|200px|right]]
 '''Hiroyuki Nakai''' is an assistant professor at Food Glycoscience Laboratory in Graduate School of Science and Technology, Niigata University. He obtained Ph. D from Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University in 2005, under the supervision of Prof. Atsuo Kimura and Prof. Seiya Chiba. He joined the group of Prof. Birte Svensson at Enzyme and Protein Chemistry, Department of Systems Biology, the Technical University of Denmark as post-doctoral fellow (2007) and assistant professor (2008-2010), working on chemoenzymatic synthesis of oligosaccharides by glycoside hydrolase of [[GH36]] <cite>Nakai2010a</cite> and phosphorylases of [[GH94]] <cite>Nakai2010b</cite> and [[GH65]] <cite>Nakai2010c Nakai2010d Nakai2009</cite> catalysing transglycosylation and reverse phosphorolysis, respectively. His recent research interests concern structure and function of Carbohydrate Active enZymes, enzymatic synthesis of oligosaccharides showing healthful effect on human intestine, antibacterial activity, and immunostimulatory activity.
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 #Nakai2009 pmid=19919544
 </biblio>

@@ Line 1: / Line 1: @@
-'''Hiroyuki Nakai''' is an assistant professor at Food Glycoscience Laboratory in Graduate School of Science and Technology, Niigata University. He obtained Ph. D from Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University in 2005, under the supervision of Prof. Atsuo Kimura and Prof. Seiya Chiba. He joined the group of Prof. Birte Svensson at Enzyme and Protein Chemistry, Department of Systems Biology, the Technical University of Denmark as post-doctoral fellow (2007) and assistant professor (2008-2010), working on chemoenzymatic synthesis of oligosaccharides by glycoside hydrolase  and phosphorylase catalysing transglycosylation and reverse phosphorolysis, respectively. His recent research interests concern structure and function of Carbohydrate Active enZymes, enzymatic synthesis of oligosaccharides showing healthful effect on human intestine, antibacterial activity, and immunostimulatory activity.
+[[Image:Nakai.jpg|thumb|200px|right]]
-* Nakai H., Baumann M.J., Petersen B.O., Westphal Y., Abou Hachem M., Dilokpimol A., Duus J.Ø., Schols H.A., Svensson B.　''Aspergillus nidulans'' α-galactosidase of glycoside hydrolase family 36 catalyses the formation of α-galacto-oligosaccharides by transglycosylation. The FEBS Journal (2010) 277, 3538–3551.
+----
-* Nakai H., Petersen B.O., Westphal Y., Dilokpimol A., Abou Hachem M., Duus J.Ø., Schols H.A., Svensson B. Rational engineering of ''Lactobacillus acidophilus'' NCFM maltose phosphorylase into either trehalose or kojibiose dual specificity phosphorylase. Protein Engineering Design & Selection (2010) 23, 781–787.
+<biblio>
+#Nakai2010a pmid=20681989
-* Nakai H., Dilokpimol A., Abou Hachem M., Svensson B. Efficient one-pot enzymatic synthesis of α-(1→4)-glucosidic disaccharides through a coupled reaction catalysed by ''Lactobacillus acidophilus'' NCFM maltose phosphorylase. Carbohydrate Research (2010) 345, 1061–1064.
+#Nakai2010b pmid=20678539
+#Nakai2010c pmid=20713411
-* Nakai H., Baumann M.J., Petersen B.O., Westphal Y., Schols H.A., Dilokpimol A., Abou Hachem M., Lahtinen S.J., Duus J.Ø., Svensson B. The maltodextrin transport system and metabolism in ''Lactobacillus acidophilus'' NCFM and production of novel α-glucosides through reverse phosphorolysis by maltose phosphorylase. The FEBS Journal (2009) 276, 7353–7365.
+#Nakai2010d pmid=20392438