User:Marcelo Liberato

Marcelo Liberato is a postdoctoral fellow in the Brazilian Bioethanol Science and Technology Laboratory (CTBE). He obtained his B. Sc. in Biology from the Federal University of São Carlos and his master and PhD were obtained in Biomolecular Physics at University of São Paulo, under the supervision of Prof. Igor Polikarpov. His work is focused on multimodular enzymes, trying to comprehend the influences of accessory modules on catalytic domains. He has determined the crystal structures of

Glycoside Hydrolases:

• GH5 Bacillus licheniformis endoglucanase [1]

• GH12 Trichoderma harzianum endoglucanase [2]

• GH62 Aspergillus nidulans arabinofuranosidase [3]

Carbohydrate-binding modules:

• CBM46 Bacillus licheniformis [1]

• CBM81 from sugar cane soil metagenome [4]

References

2. Prates ÉT, Stankovic I, Silveira RL, Liberato MV, Henrique-Silva F, Pereira N, Polikarpov I and Skaf MS. X-ray structure and molecular dynamics simulations of endoglucanase 3 from trichoderma harzianum: structural organization and substrate recognition by endoglucanases that lack cellulose binding module. Plos One. 2013 Mar;8(3):e59069. DOI:10.1371/journal.pone.0059069 | PubMed ID:23516599 | HubMed [Prates2013].

3. Contesini FJ, Liberato MV, Rubio MV, Calzado F, Zubieta MP, Riaño-Pachón DM, Squina FM, Bracht F, Skaf MS and Damasio AR. Structural and functional characterization of a highly secreted α-l-arabinofuranosidase (GH62) from Aspergillus nidulans grown on sugarcane bagasse. Biochim Biophys Acta. 2017 Dec;1865(12):1758-1769. DOI: 10.1016/j.bbapap.2017.09.001 | PubMed ID:28890404 | HubMed [Contesini2017].

4. Campos BM, Liberato MV, Alvarez TM, Zanphorlin LM, Ematsu GC, Barud H, Polikarpov I, Ruller R, Gilbert HJ, Zeri AC and Squina FM. A Novel Carbohydrate-binding Module from Sugar Cane Soil Metagenome Featuring Unique Structural and Carbohydrate Affinity Properties. J Biol Chem. 2016 Nov 4;291(45):23734-23743. DOI:10.1074/jbc.M116.744383 | PubMed ID:27621314 | HubMed [Campos2017].

1. Liberato MV, Silveira RL, Prates ÉT, de Araujo EA, Pellegrini VO, Camilo CM, Kadowaki MA, Neto Mde O, Popov A, Skaf MS, and Polikarpov I. (2016). Molecular characterization of a family 5 glycoside hydrolase suggests an induced-fit enzymatic mechanism. Sci Rep. 2016;6:23473. DOI:10.1038/srep23473 |