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Difference between revisions of "User:Birgitte Zeuner"
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| − | + | '''Assistant Professor''' at the [https://www.bioengineering.dtu.dk/english Department of Biotechnology and Biomedicine], [https://www.dtu.dk Technical University of Denmark]. | |
| − | + | == Background == | |
| − | |||
| − | |||
| − | + | I obtained an MSc degree in Biotechnology at [https://www.kt.dtu.dk Department of Chemical and Biochemical Engineering], [https://www.dtu.dk Technical University of Denmark] in 2009. I continued my PhD studies there under the supervision of Anne S. Meyer, where I worked with [[CE1]] feruloyl esterases to catalyze transesterification in ionic liquids <cite>Zeuner2011 Zeuner2012</cite>, as well as with [[GH33]] sialidases for transsialylation <cite>Zeuner2014a Zeuner2014b</cite>. Starting from solvent engineering and reaction design to improve enzyme-catalysed synthesis, my work has since evolved to include protein engineering and enzyme discovery. My post doc studies included a lot of carbohydrate analysis (HPAEC-PAD) and work on [[GH33]] sialidases <cite>Zeuner2016a Nordvang2016 Zeuner2017</cite>, [[GH29]] α-L-fucosidases <cite>Zeuner2018a Zeuner2018b Zeuner2020</cite> , [[GH20]] β-N-acetylhexosaminidases <cite>Nyffenegger2015 Jamek2018</cite>, and other GH families <cite>Zeuner2016b</cite> for transglycosylation to yield human milk oligosaccharide structures. In 2018, I was appointed Assistant Professor in Enzyme Technology for Oligosaccharide Synthesis at [https://www.bioengineering.dtu.dk/english Department of Biotechnology and Biomedicine], [https://www.dtu.dk Technical University of Denmark]. My current work focuses on transglycosylation <cite>Zeuner2014c, Zeuner2019, Muschiol2020, Zeuner2020</cite>. As GH-catalysed synthesis is not always a walk in the park, I sometimes spend time off from that to deal with targeted carbohydrate breakdown using [[PL1]] pectin lyases <cite>Chung2017</cite>. | |
| + | == Selected papers == | ||
| + | <biblio> | ||
| + | |||
| + | #Zeuner2011 Zeuner, B., Ståhlberg, T., van Buu, O.N., Kunov-Kruse, A.J., Riisager, A., Meyer, A.S. (2011) Dependency of the hydrogen bonding capacity of the solvent anion on the thermal stability of feruloyl esterases in ionic liquid systems. Green Chem. 13, 1550-1557.[https://doi.org/10.1039/C1GC15115K DOI: 10.1039/C1GC15115K] | ||
| + | #Zeuner2012 pmid=22154740 | ||
| + | #Zeuner2014a pmid=24411449 | ||
| + | #Zeuner2014b pmid=24563319 | ||
| + | #Zeuner2016a pmid=26672447 | ||
| + | #Nordvang2016 pmid=27367145 | ||
| + | #Zeuner2017 Zeuner, B., González-Delgado, I., Holck, J., Morales, G., López-Muñoz, M.J., Segura, Y., Meyer, A.S., Mikkelsen, J.D. (2017) Characterization and immobilization of engineered sialidases from Trypanosoma rangeli for transsialylation. AIMS Mol. Sci. 4(2), 140-163.[https://doi.org/10.3934/molsci.2017.2.140 DOI: 10.3934/molsci.2017.2.140] | ||
| + | #Muschiol2020 Muschiol, J., Vuillemin, M., Meyer, A.S., Zeuner, B. (2020) β-N-Acetylhexosaminidases for carbohydrate synthesis via trans-glycosylation. Catalysts 10, 365.[https://doi.org/10.3390/catal10040365 DOI: 10.3390/catal10040365] | ||
| + | #Jamek2018 pmid=29911342 | ||
| + | #Nyffenegger2015 pmid=25843303 | ||
| + | #Zeuner2019 pmid=31141914 | ||
| + | #Zeuner2014c pmid=25208138 | ||
| + | |||
| + | #Zeuner2018a pmid=29221760 | ||
| + | #Zeuner2018b pmid=29859601 | ||
| + | |||
| + | #Zeuner2020 Zeuner, B., Meyer, A.S. (2020) Enzymatic transfucosylation for synthesis of human milk oligosaccharides. Carbohydr. Res.[https://doi.org/10.1016/j.carres.2020.108029 DOI: 10.1016/j.carres.2020.108029] | ||
| − | + | #Chung2017 pmid=29029078 | |
| − | + | #Zeuner2016b pmid=26802542 | |
| − | # | ||
</biblio> | </biblio> | ||
| − | |||
<!-- Do not remove this Category tag --> | <!-- Do not remove this Category tag --> | ||
[[Category:Contributors|Zeuner,Birgitte]] | [[Category:Contributors|Zeuner,Birgitte]] | ||
Revision as of 20:09, 14 May 2020
Assistant Professor at the Department of Biotechnology and Biomedicine, Technical University of Denmark.
Background
I obtained an MSc degree in Biotechnology at Department of Chemical and Biochemical Engineering, Technical University of Denmark in 2009. I continued my PhD studies there under the supervision of Anne S. Meyer, where I worked with CE1 feruloyl esterases to catalyze transesterification in ionic liquids [1, 2], as well as with GH33 sialidases for transsialylation [3, 4]. Starting from solvent engineering and reaction design to improve enzyme-catalysed synthesis, my work has since evolved to include protein engineering and enzyme discovery. My post doc studies included a lot of carbohydrate analysis (HPAEC-PAD) and work on GH33 sialidases [5, 6, 7], GH29 α-L-fucosidases [8, 9, 10] , GH20 β-N-acetylhexosaminidases [11, 12], and other GH families [13] for transglycosylation to yield human milk oligosaccharide structures. In 2018, I was appointed Assistant Professor in Enzyme Technology for Oligosaccharide Synthesis at Department of Biotechnology and Biomedicine, Technical University of Denmark. My current work focuses on transglycosylation [10, 14, 15, 16]. As GH-catalysed synthesis is not always a walk in the park, I sometimes spend time off from that to deal with targeted carbohydrate breakdown using PL1 pectin lyases [17].
Selected papers
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Zeuner, B., Ståhlberg, T., van Buu, O.N., Kunov-Kruse, A.J., Riisager, A., Meyer, A.S. (2011) Dependency of the hydrogen bonding capacity of the solvent anion on the thermal stability of feruloyl esterases in ionic liquid systems. Green Chem. 13, 1550-1557.DOI: 10.1039/C1GC15115K
- Zeuner B, Kontogeorgis GM, Riisager A, and Meyer AS. (2012). Thermodynamically based solvent design for enzymatic saccharide acylation with hydroxycinnamic acids in non-conventional media. N Biotechnol. 2012;29(3):255-70. DOI:10.1016/j.nbt.2011.11.011 |
- Zeuner B, Luo J, Nyffenegger C, Aumala V, Mikkelsen JD, and Meyer AS. (2014). Optimizing the biocatalytic productivity of an engineered sialidase from Trypanosoma rangeli for 3'-sialyllactose production. Enzyme Microb Technol. 2014;55:85-93. DOI:10.1016/j.enzmictec.2013.12.009 |
- Zeuner B, Riisager A, Mikkelsen JD, and Meyer AS. (2014). Improvement of trans-sialylation versus hydrolysis activity of an engineered sialidase from Trypanosoma rangeli by use of co-solvents. Biotechnol Lett. 2014;36(6):1315-20. DOI:10.1007/s10529-014-1488-3 |
- Zeuner B, Holck J, Perna V, Mikkelsen JD, and Meyer AS. (2016). Quantitative enzymatic production of sialylated galactooligosaccharides with an engineered sialidase from Trypanosoma rangeli. Enzyme Microb Technol. 2016;82:42-50. DOI:10.1016/j.enzmictec.2015.08.010 |
- Nordvang RT, Nyffenegger C, Holck J, Jers C, Zeuner B, Sundekilde UK, Meyer AS, and Mikkelsen JD. (2016). It All Starts with a Sandwich: Identification of Sialidases with Trans-Glycosylation Activity. PLoS One. 2016;11(7):e0158434. DOI:10.1371/journal.pone.0158434 |
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Zeuner, B., González-Delgado, I., Holck, J., Morales, G., López-Muñoz, M.J., Segura, Y., Meyer, A.S., Mikkelsen, J.D. (2017) Characterization and immobilization of engineered sialidases from Trypanosoma rangeli for transsialylation. AIMS Mol. Sci. 4(2), 140-163.DOI: 10.3934/molsci.2017.2.140
- Zeuner B, Muschiol J, Holck J, Lezyk M, Gedde MR, Jers C, Mikkelsen JD, and Meyer AS. (2018). Substrate specificity and transfucosylation activity of GH29 α-l-fucosidases for enzymatic production of human milk oligosaccharides. N Biotechnol. 2018;41:34-45. DOI:10.1016/j.nbt.2017.12.002 |
- Zeuner B, Vuillemin M, Holck J, Muschiol J, and Meyer AS. (2018). Loop engineering of an α-1,3/4-l-fucosidase for improved synthesis of human milk oligosaccharides. Enzyme Microb Technol. 2018;115:37-44. DOI:10.1016/j.enzmictec.2018.04.008 |
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Zeuner, B., Meyer, A.S. (2020) Enzymatic transfucosylation for synthesis of human milk oligosaccharides. Carbohydr. Res.DOI: 10.1016/j.carres.2020.108029
- Nyffenegger C, Nordvang RT, Zeuner B, Łężyk M, Difilippo E, Logtenberg MJ, Schols HA, Meyer AS, and Mikkelsen JD. (2015). Backbone structures in human milk oligosaccharides: trans-glycosylation by metagenomic β-N-acetylhexosaminidases. Appl Microbiol Biotechnol. 2015;99(19):7997-8009. DOI:10.1007/s00253-015-6550-0 |
- Jamek SB, Muschiol J, Holck J, Zeuner B, Busk PK, Mikkelsen JD, and Meyer AS. (2018). Loop Protein Engineering for Improved Transglycosylation Activity of a β-N-Acetylhexosaminidase. Chembiochem. 2018;19(17):1858-1865. DOI:10.1002/cbic.201800181 |
- Zeuner B, Nyffenegger C, Mikkelsen JD, and Meyer AS. (2016). Thermostable β-galactosidases for the synthesis of human milk oligosaccharides. N Biotechnol. 2016;33(3):355-60. DOI:10.1016/j.nbt.2016.01.003 |
- Zeuner B, Jers C, Mikkelsen JD, and Meyer AS. (2014). Methods for improving enzymatic trans-glycosylation for synthesis of human milk oligosaccharide biomimetics. J Agric Food Chem. 2014;62(40):9615-31. DOI:10.1021/jf502619p |
- Zeuner B, Teze D, Muschiol J, and Meyer AS. (2019). Synthesis of Human Milk Oligosaccharides: Protein Engineering Strategies for Improved Enzymatic Transglycosylation. Molecules. 2019;24(11). DOI:10.3390/molecules24112033 |
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Muschiol, J., Vuillemin, M., Meyer, A.S., Zeuner, B. (2020) β-N-Acetylhexosaminidases for carbohydrate synthesis via trans-glycosylation. Catalysts 10, 365.DOI: 10.3390/catal10040365
- Chung WSF, Meijerink M, Zeuner B, Holck J, Louis P, Meyer AS, Wells JM, Flint HJ, and Duncan SH. (2017). Prebiotic potential of pectin and pectic oligosaccharides to promote anti-inflammatory commensal bacteria in the human colon. FEMS Microbiol Ecol. 2017;93(11). DOI:10.1093/femsec/fix127 |