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Difference between revisions of "Glycoside Hydrolase Family 34"
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| − | * [[Author]]: ^^^ | + | * [[Author]]: ^^^Kyle Robinson^^^ |
* [[Responsible Curator]]: ^^^Steve Withers^^^ | * [[Responsible Curator]]: ^^^Steve Withers^^^ | ||
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|- | |- | ||
|'''Clan''' | |'''Clan''' | ||
| − | |GH- | + | |GH-E |
|- | |- | ||
|'''Mechanism''' | |'''Mechanism''' | ||
| − | |retaining | + | |retaining |
|- | |- | ||
|'''Active site residues''' | |'''Active site residues''' | ||
| − | | | + | |Tyr/Glu |
|- | |- | ||
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link''' | |{{Hl2}} colspan="2" align="center" |'''CAZy DB link''' | ||
| Line 47: | Line 47: | ||
== Family Firsts == | == Family Firsts == | ||
| − | ;First stereochemistry determination: | + | ;First stereochemistry determination: Determined for influenza NA by NMR by the von Itzstein group Eur. J. Biochem. (1992) 207, 335-343. <cite>Comfort2007</cite>. |
| − | ;First catalytic nucleophile identification: | + | ;First catalytic nucleophile identification: Implied by X-ray structures, though intermediate thought to be a carbocation. Determined definitively using a 2,3-difluorosialic acid in the Withers group Kim et al Science (2013) 340, 71-75. <cite>Sinnott1990</cite>. |
| − | ;First general acid/base residue identification: | + | ;First general acid/base residue identification: Inferred from X-ray structure below <cite>He1999</cite>. |
| − | ;First 3-D structure: | + | ;First 3-D structure: Influenza neuraminidase determined by Colman group: Varghese et al (1983) Nature 303, 35-40. <cite>StickWilliams</cite>. |
== References == | == References == | ||
Revision as of 16:55, 15 June 2020
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author: ^^^Kyle Robinson^^^
- Responsible Curator: ^^^Steve Withers^^^
| Glycoside Hydrolase Family GH34 | |
| Clan | GH-E |
| Mechanism | retaining |
| Active site residues | Tyr/Glu |
| CAZy DB link | |
| https://www.cazy.org/GH34.html | |
Substrate specificities
Content is to be added here.
This is an example of how to make references to a journal article [1]. (See the References section below). Multiple references can go in the same place like this [1, 2]. You can even cite books using just the ISBN [3]. References that are not in PubMed can be typed in by hand [4].
Kinetics and Mechanism
Content is to be added here.
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Content is to be added here.
Family Firsts
- First stereochemistry determination
- Determined for influenza NA by NMR by the von Itzstein group Eur. J. Biochem. (1992) 207, 335-343. [1].
- First catalytic nucleophile identification
- Implied by X-ray structures, though intermediate thought to be a carbocation. Determined definitively using a 2,3-difluorosialic acid in the Withers group Kim et al Science (2013) 340, 71-75. [4].
- First general acid/base residue identification
- Inferred from X-ray structure below [2].
- First 3-D structure
- Influenza neuraminidase determined by Colman group: Varghese et al (1983) Nature 303, 35-40. [3].
References
- Comfort DA, Bobrov KS, Ivanen DR, Shabalin KA, Harris JM, Kulminskaya AA, Brumer H, and Kelly RM. (2007). Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Biochemistry. 2007;46(11):3319-30. DOI:10.1021/bi061521n |
- He S and Withers SG. (1997). Assignment of sweet almond beta-glucosidase as a family 1 glycosidase and identification of its active site nucleophile. J Biol Chem. 1997;272(40):24864-7. DOI:10.1074/jbc.272.40.24864 |
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Sinnott, M.L. (1990) Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90, 1171-1202. DOI: 10.1021/cr00105a006