Carbohydrate Binding Module Family 19

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• Author: ^^^Elizabeth Ficko-Blean^^^
• Responsible Curator: ^^^Elizabeth Ficko-Blean^^^

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CAZy DB link

https://www.cazy.org/CBMnn.html

Ligand specificities

The CBM19 found in the CTS1 chitinase produced by Saccharomyces cerevisiae has been characterized as a chitin-binding CBM [1].

Mention here all major natural ligand specificities that are found within a given family (also plant or mammalian origin). Certain linkages and promiscuity would also be mentioned here if biologically relevant.

Note: Here is an example of how to insert references in the text, together with the "biblio" section below: Please see these references for an essential introduction to the CAZy classification system: [2, 3]. CBMs, in particular, have been extensively reviewed [4, 5, 6, 7, 8].

Structural Features

Content in this section should include, in paragraph form, a description of:

• Fold: Structural fold (beta trefoil, beta sandwich, etc.)
• Type: Include here Type A, B, or C and properties
• Features of ligand binding: Describe CBM binding pocket location (Side or apex) important residues for binding (W, Y, F, subsites), interact with reducing end, non-reducing end, planar surface or within polysaccharide chains. Include examples pdb codes. Metal ion dependent. Etc.

Functionalities

Chitin is an important component of the cell wall of Saccharomyces cerevisiae. It is specifically located at the junction of mother and daughter cells providing mechanical stability. The CTS1 enzyme produced by S. cerevisiae hydrolyses chitin [9, 10] and has a role in cell separation [1].

Content in this section should include, in paragraph form, a description of:

• Functional role of CBM: Describe common functional roles such as targeting, disruptive, anchoring, proximity/position on substrate.
• Most Common Associated Modules: 1. Glycoside Hydrolase Activity; 2. Additional Associated Modules (other CBM, FNIII, cohesin, dockerins, expansins, etc.)
• Novel Applications: Include here if CBM has been used to modify another enzyme, or if a CBM was used to label plant/mammalian tissues? Etc.

Family Firsts

First Identified

Insert archetype here, possibly including very brief synopsis.

First Structural Characterization

Insert archetype here, possibly including very brief synopsis.

References

1. Kuranda MJ and Robbins PW. (1991). Chitinase is required for cell separation during growth of Saccharomyces cerevisiae. J Biol Chem. 1991;266(29):19758-67. | Google Books | Open Library
9. Correa JU, Elango N, Polacheck I, and Cabib E. (1982). Endochitinase, a mannan-associated enzyme from Saccharomyces cerevisiae. J Biol Chem. 1982;257(3):1392-7. | Google Books | Open Library
10. Kuranda MJ and Robbins PW. (1987). Cloning and heterologous expression of glycosidase genes from Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1987;84(9):2585-9. DOI:10.1073/pnas.84.9.2585 |