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Difference between revisions of "Carbohydrate Binding Module Family 44"
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== Ligand specificities == | == Ligand specificities == | ||
| − | + | CBM44 targets ß-1,4-polymers such as xyloglucan and cellulose (hydroxyethylcellulose and Avicel), mixed linkage ß-1,3/ß1,4- glucans (lichenan and barley) or glucomannan (konjac) <cite>Najmudin2006</cite>. Affinity for xylan was very low and binding to laminarin, curdlan, pullulan, pustulan, galactomannan or galactan was negative. Isothermal titration calorimetry (ITC) revealed highest affinity for xyloglucan as a polysaccharide, which was comparable to the affinity for cellohexaose as an oligosaccharide. For other cello-oligosaccharides, this affinity decreased with decreasing chain length, while no binding was detected for cellotriose. | |
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== Structural Features == | == Structural Features == | ||
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== References == | == References == | ||
<biblio> | <biblio> | ||
| − | # | + | #Najmudin2006 pmid=16314409 |
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</biblio> | </biblio> | ||
[[Category:Carbohydrate Binding Module Families|CBM044]] <!-- ATTENTION: Make sure to replace "nnn" with a three digit family number, e.g. "032" or "105" etc., for proper sorting of the page by family number. --> | [[Category:Carbohydrate Binding Module Families|CBM044]] <!-- ATTENTION: Make sure to replace "nnn" with a three digit family number, e.g. "032" or "105" etc., for proper sorting of the page by family number. --> | ||
Revision as of 07:11, 6 January 2023
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| CAZy DB link | |
| https://www.cazy.org/CBMnn.html |
Ligand specificities
CBM44 targets ß-1,4-polymers such as xyloglucan and cellulose (hydroxyethylcellulose and Avicel), mixed linkage ß-1,3/ß1,4- glucans (lichenan and barley) or glucomannan (konjac) [1]. Affinity for xylan was very low and binding to laminarin, curdlan, pullulan, pustulan, galactomannan or galactan was negative. Isothermal titration calorimetry (ITC) revealed highest affinity for xyloglucan as a polysaccharide, which was comparable to the affinity for cellohexaose as an oligosaccharide. For other cello-oligosaccharides, this affinity decreased with decreasing chain length, while no binding was detected for cellotriose.
Structural Features
Content in this section should include, in paragraph form, a description of:
- Fold: Structural fold (beta trefoil, beta sandwich, etc.)
- Type: Include here Type A, B, or C and properties
- Features of ligand binding: Describe CBM binding pocket location (Side or apex) important residues for binding (W, Y, F, subsites), interact with reducing end, non-reducing end, planar surface or within polysaccharide chains. Include examples pdb codes. Metal ion dependent. Etc.
Functionalities
Content in this section should include, in paragraph form, a description of:
- Functional role of CBM: Describe common functional roles such as targeting, disruptive, anchoring, proximity/position on substrate.
- Most Common Associated Modules: 1. Glycoside Hydrolase Activity; 2. Additional Associated Modules (other CBM, FNIII, cohesin, dockerins, expansins, etc.)
- Novel Applications: Include here if CBM has been used to modify another enzyme, or if a CBM was used to label plant/mammalian tissues? Etc.
Family Firsts
- First Identified
- Insert archetype here, possibly including very brief synopsis.
- First Structural Characterization
- Insert archetype here, possibly including very brief synopsis.