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| − | '''November 23, 2018:''' ''Welcome to the CAZypedia fold [[CBM49]]!'' The crystalline cellulose-binding [[CBM49]] CAZypedia page was authored by '''[[User:Breeanna Urbanowicz|Breeanna Urbanowicz]]''' and '''[[User:Elizabeth Ficko-Blean|Elizabeth Ficko-Blean]]'''. '''[[User:Breeanna Urbanowicz|Breeanna Urbanowicz]]''' also acted as responsible curator. There is experimental evidence that rice[[CBM49]] is cleaved post-translationally in vivo which probably plays an important role in plant growth. Find out more about the interesting family 49 CBMs '''[[CBM49|here]]'''. | + | '''19 July 2024:''' ''Chalk-up one more for the GTs!'' The '''[[Glycosyltransferase Family 47]]''' page joined the small group of [[Curator Approved]] [[Glycosyltransferase Families]] pages in ''CAZypedia'' today. This entry was [[author]]ed by Ph.D. students '''[[User:Daniel Tehrani|Daniel Tehrani]]''' and '''[[User:Charlie Corulli|Charlie Corulli]]''', and [[Responsible Curator|Curated]] by '''[[User:Breeanna Urbanowicz|Breeanna Urbanowicz]]''' with input from '''[https://ccrc.uga.edu/team/kelley-moremen/ Kelley Moremen]'''. Widely represented in plants, '''[[GT47]]''' members are anomer-[[inverting]] [[glycosyltransferases]], which are involved in the biosynthesis of several cell wall matrix polysaccharides. Representatives from mammals are involved in heparin biosynthesis. Correspondingly, members of [[GH47]] have diverse substrate specificities, including the transfer of both anionic and neutral monosaccharides to polysaccharides. ''This is a great example where two keen Ph.D. students worked with their supervisors to create a valuable page for the scientific community. We encourage others to follow their lead, on your favorite family!'' |
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| − | '''October 11, 2018:''' ''Fall ushers in a new CAZypedia CBM family page.'' The chitin-binding and lytic polysaccharide monooxygenase associated [[CBM73]] family is described in detail. '''[[User:Zarah Forsberg|Zarah Forsberg]]''' authored the page and '''[[User:Gustav Vaaje-Kolstad|Gustav Vaaje-Kolstad]]''' acted as responsible curator. Learn more about the [[CBM73]] family on its CAZypedia [[CBM73|page]].
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| − | '''September 24, 2018:''' ''Revenge of the Ruminococci Part Deux.'' Two more CBM families from Ruminococcal bacteria are ready for reading. The [[CBM79]] and [[CBM80]] CAZypedia pages were authored by '''[[User:Immacolata Venditto|Immacolata Venditto]]''' and '''[[User:Harry Gilbert|Harry Gilbert]]''' acted as responsible curator. These CBMs are important for enzyme targeting but also for targeting the entire cellulosome complex to substrate. Information on the [[CBM79]] and [[CBM80]] families can be found on their respective CAZypedia pages. | |
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| − | '''July 3, 2018:''' ''Revenge of the Ruminococci.'' Three CBM families containing characterized CBM members from Ruminococcal bacteria are now on-line in CAZypedia; two of these families contain uniquely Ruminococcal CBMs. '''[[User:Ana Luis|Ana Luis]]''' authored the pages (in one fell swoop) and '''[[User:Harry Gilbert|Harry Gilbert]]''' acted as responsible curator. Descriptions of families '''[[CBM75]]''', '''[[CBM76]]''' and '''[[CBM77]]''' can be found on their respective CAZypedia pages.
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| − | '''June 4, 2018:''' ''When two worlds collide.'' The '''[[CBM81]]''' family has an interesting binding mechanism, mixing characteristics of both [[Carbohydrate-binding_modules#Types|type A]] and [[Carbohydrate-binding_modules#Types|type B]] CBMs. The binding is enthalpically driven to soluble ligands, so by definition this is a [[Carbohydrate-binding_modules#Types|type B]] interaction; however, the CBM binding face resembles the flat face of [[Carbohydrate-binding_modules#Types|type A]] (crystalline-polysaccharide binding) CBMs. '''[[User: Marcelo Liberato|Marcelo Liberato]]''' [[author]]ed the [[CBM81]] page and '''[[User: Fabio Squina|Fabio Squina]]''' acted as the [[Responsible Curator]]. Find out more about the unusual family 81 CBMs '''[[CBM81|here]]'''.
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| | + | '''9 July 2024:''' ''Yet another new family of beta-1,2-glucan-active enzymes!'' Today, '''[[User:Masahiro Nakajima|Masahiro Nakajima]]''' [[Curator Approved]] the '''[[Glycoside Hydrolase Family 186]]''' page by '''[[User:Sei Motouchi|Sei Motouchi]]'''. '''[[GH186]]''' is a family of anomer-[[inverting]] enzymes from bacteria, members of which are specific for beta-1,2-glucans. Intriguingly, although some [[GH186]] members work as classic [[glycoside hydrolases]], others perform transglycosylation by wrapping the sugar chain around in the active-site, to position the 6-OH group of a terminal glucosyl unit for direct attack. Also notable, [[GH186]] members appear to use an extended chain of water molecules to relay acceptor deprotonation by the [[general base]] residue, ''i.e.'' a [https://en.wikipedia.org/wiki/Grotthuss_mechanism Grotthuss mechanism]. ''Check out the '''[[GH186]]''' page to learn more about these interesting enzymes, and make sure to see the [[GH189]], [[GH144]], and [[GH162]] pages from this same group.'' |
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| − | '''25 May 2018:''' ''The almost exclusive expansin associated [[CBM63]] family is on-line.'' An interesting function is described as a bacterial [[CBM63]] targets expansin to biomechanical hotspots in the ''Arabidopsis'' cell wall, where cell wall loosening occurs. The page was authored by '''[[User: Will Chase|Will Chase]]''' and '''[[User: Daniel Cosgrove|Daniel Cosgrove]]''' with '''[[User: Daniel Cosgrove|Daniel Cosgrove]]''' acting as responsible curator. Learn more about this expansin family CBM [[CBM63|here]].
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| − | '''4 May 2018:''' ''CAZypedia's first non-LPMO Auxiliary Activity Family page!'' Today [[Responsible Curator]] '''[[User:Roland Ludwig|Roland Ludwig]]''' [[Curator Approved|approved]] '''[[User:Daniel Kracher|Daniel Kracher's]]''' and his expansive '''[[Auxiliary Activity Family 3]]''' page. '''[[AA3]]''' comprises a number of FAD-dependent redox enzymes including cellobiose dehydrogenase, aryl alcohol oxidase/dehydrogenases, glucose oxidases and glucose dehydrogenases, pyranose dehydrogenase, alcohol oxidase, and pyranose oxidase across four subfamilies. '''''[[User:Roland Ludwig|Roland]]''' and '''[[User:Daniel Kracher|Daniel]]''' have done a monumental job in succinctly capturing the diversity of this family, which you can read about [[Auxiliary Activity Family 3|here]].''
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| − | '''2 May 2018:''' ''The [[CBM65]] page has been added to the CAZypedia fold.'' This is a small CAZy family with two currently characterized members from an anaerobic cellulolytic ruminal bacterium. The two [[CBM65]] members bind various beta-glucans and play an important role in enhancing enzymatic activity on substrate. The page was authored by '''[[User:Ana Luis|Ana Luis]]''' and '''[[User:Harry Gilbert|Harry Gilbert]]''' acted as responsible curator. ''Learn more about this CBM family [[CBM65|here]].''
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| − | '''1 March 2018:''' The shortest month of the year saw four '''[[CBM]]''' families reach '''[[Curator Approved]]''' status, including two early members. '''[[User:Harry Gilbert|Harry Gilbert]]''' with input from '''[[User:Ed Bayer|Ed Bayer]]''', who also acted as '''[[Responsible Curator]]''', authored the cellulose-binding '''[[CBM3]]''' page. '''[[User:Harry Gilbert|Harry Gilbert]]''' and '''[[User:Claire Dumon|Claire Dumon]]''' both contributed to the xylan and glucan-binding '''[[CBM4]]''' page. The xylan-binding '''[[CBM22]]''' page was taken on by '''[[User:Harry Gilbert|Harry Gilbert]]''' solo. Finally, the cellulose-binding '''[[CBM78]]''' family was authored by '''[[User:Immacolata Venditto|Immacolata Venditto]]''', with '''[[User:Harry Gilbert|Harry Gilbert]]''' acting as '''[[Responsible Curator]]'''. ''Learn more about each of these families on [[Carbohydrate Binding Module Families|their respective pages]].''
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| − | '''15 February 2018:''' ''More on pectin, and also arabinan:'' '''[[User:Jonathon Briggs|Jonathon Briggs]]''' recently completed the '''[[Glycoside Hydrolase Family 147]]''' and '''[[Glycoside Hydrolase Family 146]]''' pages, which are involved in the utilization of pectin and galactan, respectively, by human gut Bacteroidetes. Both pages were upgraded to [[Curator Approved]] status today by [[Responsible Curator]] '''[[User:Harry Gilbert|Harry Gilbert]]'''. ''Learn more about these newly described families at [[GH146]] and [[GH147]].''
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| − | '''13 February 2018:''' ''The intricacies of pectin deconstruction:'' Rhamnogalacturonan II (RGII) represents the most structurally complex plant cell wall polysaccharide currently known, the complete saccharification of which requires a battery of CAZymes. Under the guidance of [[Responsible Curator]] '''[[User:Harry Gilbert|Harry Gilbert]]''', four new GH pages related to RGII deconstruction were [[Curator Approved]] today. Special thanks go to [[Author]]s '''[[User:Ana Luis|Ana Luis]]''' ('''[[GH106]]''', '''[[GH139]]''', and '''[[GH141]]''') and '''[[User:Didier Ndeh|Didier Ndeh]]''' ('''[[GH138]]''') for their hard work in putting these pages together. ''Learn more about the individual, specific contributions of each of these families (three of which have been recently uncovered) to microbial RGII utilization on their respective pages.'' | + | '''2 May 2024:''' ''CBDs I to X... A major milestone!'' '''CBM families 1 to 10 are now complete!''' These are the old CBD (cellulose-binding domain) families, which used to have roman numerals as part of their nomenclature. A special thank you to all the authors and responsible curators who have contributed to this major milestone. Go have a peek at each of these old school families on their respective ''CAZypedia'' pages: '''[[CBM1]], [[CBM2]], [[CBM3]], [[CBM4]], [[CBM5]], [[CBM6]], [[CBM7]], [[CBM8]], [[CBM9]], and [[CBM10]]'''. |
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19 July 2024: Chalk-up one more for the GTs! The Glycosyltransferase Family 47 page joined the small group of Curator Approved Glycosyltransferase Families pages in CAZypedia today. This entry was authored by Ph.D. students Daniel Tehrani and Charlie Corulli, and Curated by Breeanna Urbanowicz with input from Kelley Moremen. Widely represented in plants, GT47 members are anomer-inverting glycosyltransferases, which are involved in the biosynthesis of several cell wall matrix polysaccharides. Representatives from mammals are involved in heparin biosynthesis. Correspondingly, members of GH47 have diverse substrate specificities, including the transfer of both anionic and neutral monosaccharides to polysaccharides. This is a great example where two keen Ph.D. students worked with their supervisors to create a valuable page for the scientific community. We encourage others to follow their lead, on your favorite family!
9 July 2024: Yet another new family of beta-1,2-glucan-active enzymes! Today, Masahiro Nakajima Curator Approved the Glycoside Hydrolase Family 186 page by Sei Motouchi. GH186 is a family of anomer-inverting enzymes from bacteria, members of which are specific for beta-1,2-glucans. Intriguingly, although some GH186 members work as classic glycoside hydrolases, others perform transglycosylation by wrapping the sugar chain around in the active-site, to position the 6-OH group of a terminal glucosyl unit for direct attack. Also notable, GH186 members appear to use an extended chain of water molecules to relay acceptor deprotonation by the general base residue, i.e. a Grotthuss mechanism. Check out the GH186 page to learn more about these interesting enzymes, and make sure to see the GH189, GH144, and GH162 pages from this same group.
2 May 2024: CBDs I to X... A major milestone! CBM families 1 to 10 are now complete! These are the old CBD (cellulose-binding domain) families, which used to have roman numerals as part of their nomenclature. A special thank you to all the authors and responsible curators who have contributed to this major milestone. Go have a peek at each of these old school families on their respective CAZypedia pages: CBM1, CBM2, CBM3, CBM4, CBM5, CBM6, CBM7, CBM8, CBM9, and CBM10.