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Difference between revisions of "Polysaccharide Lyase Family 32"
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== Substrate specificities == | == Substrate specificities == | ||
| − | + | The PL32 family was created following the functional characterization of the ORF “HMPREF1071_02769” from ''Bacteroides salyersiae ''CL02T12C01 (EIY62149.1), which showed endo-mannuronan lyase activity ([https://www.enzyme-database.org/query.php?ec=4.2.2.3 EC 4.2.2.3]) <cite>Helbert2019</cite>. Shortly afterwards, the sequence was <u>'''reclassified'''</u> as PL_nc on the basis of structural modelling revealing that the catalytic domain was restricted to the N-terminal domain ([http://www.cazy.org/PL0.html PL_nc]) <cite>Drula2022</cite>. The small number of sequences and the proximity to the PL14 and PL36 families do not allow the creation of a new PL family. | |
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| − | + | == References == | |
| − | + | <biblio> | |
| − | == | + | #Helbert2019 pmid=30850540 |
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| − | = | + | #Drula2022 pmid=34850161 |
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</biblio> | </biblio> | ||
[[Category:Polysaccharide Lyase Families|PL032]] | [[Category:Polysaccharide Lyase Families|PL032]] | ||
Revision as of 07:14, 23 August 2024
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
Substrate specificities
The PL32 family was created following the functional characterization of the ORF “HMPREF1071_02769” from Bacteroides salyersiae CL02T12C01 (EIY62149.1), which showed endo-mannuronan lyase activity (EC 4.2.2.3) [1]. Shortly afterwards, the sequence was reclassified as PL_nc on the basis of structural modelling revealing that the catalytic domain was restricted to the N-terminal domain (PL_nc) [2]. The small number of sequences and the proximity to the PL14 and PL36 families do not allow the creation of a new PL family.
References
- Helbert W, Poulet L, Drouillard S, Mathieu S, Loiodice M, Couturier M, Lombard V, Terrapon N, Turchetto J, Vincentelli R, and Henrissat B. (2019). Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space. Proc Natl Acad Sci U S A. 2019;116(13):6063-6068. DOI:10.1073/pnas.1815791116 |
- Drula E, Garron ML, Dogan S, Lombard V, Henrissat B, and Terrapon N. (2022). The carbohydrate-active enzyme database: functions and literature. Nucleic Acids Res. 2022;50(D1):D571-D577. DOI:10.1093/nar/gkab1045 |