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Difference between revisions of "Polysaccharide Lyase Family 30"
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== Substrate specificities == | == Substrate specificities == | ||
| − | + | PL30 is a small family (38 sequences), exclusively bacterial belonging to the phylum ''Bacteroidetes'' ([http://www.cazy.org/PL30.html PL30]) <cite>Drula2022</cite>. The founding member of family 30, and the only one characterized, belongs to ''Bacteroides cellulosilyticus WH2 (BcellWH2_02524, ''WP_029426181.1). The activity was measured against hyaluronic acid by following the absorbance at 232nm. The increase in absorbance confirms that ''BcellWH2_02524 ''is a hyaluronan lyase ([https://www.enzyme-database.org/query.php?ec=4.2.2.1 4.2.2.1]), probably using the by β-elimination process like all PL families <cite>Helbert2019</cite>. | |
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== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
| − | + | Not Determined | |
== Catalytic Residues == | == Catalytic Residues == | ||
| − | + | Not Determined | |
== Three-dimensional structures == | == Three-dimensional structures == | ||
| − | + | Unsolved | |
== Family Firsts == | == Family Firsts == | ||
| − | ;First | + | ;First description of catalytic activity: Hyaluronan lyase was the first activity discovered for the family PL30 <cite>Helbert2019</cite>. |
| − | ;First | + | ;First charge neutralizer identification: To be determined |
| − | ;First | + | ;First Brønstead acid and base residue identification: To be determinated |
| − | ;First 3-D structure: | + | ;First 3-D structure: To be determinated |
== References == | == References == | ||
<biblio> | <biblio> | ||
| − | # | + | |
| − | # | + | #Drula2022 pmid=34850161 |
| + | |||
| + | #Helbert2019 pmid=30850540 | ||
</biblio> | </biblio> | ||
[[Category:Polysaccharide Lyase Families|PL030]] | [[Category:Polysaccharide Lyase Families|PL030]] | ||
Latest revision as of 08:31, 23 August 2024
This page has been approved by the Responsible Curator as essentially complete. CAZypedia is a living document, so further improvement of this page is still possible. If you would like to suggest an addition or correction, please contact the page's Responsible Curator directly by e-mail.
| Polysaccharide Lyase Family PL30 | |
| 3D Structure | |
| Mechanism | |
| Charge neutraliser | |
| Active site residues | |
| CAZy DB link | |
| http://www.cazy.org/PL30.html | |
Substrate specificities
PL30 is a small family (38 sequences), exclusively bacterial belonging to the phylum Bacteroidetes (PL30) [1]. The founding member of family 30, and the only one characterized, belongs to Bacteroides cellulosilyticus WH2 (BcellWH2_02524, WP_029426181.1). The activity was measured against hyaluronic acid by following the absorbance at 232nm. The increase in absorbance confirms that BcellWH2_02524 is a hyaluronan lyase (4.2.2.1), probably using the by β-elimination process like all PL families [2].
Kinetics and Mechanism
Not Determined
Catalytic Residues
Not Determined
Three-dimensional structures
Unsolved
Family Firsts
- First description of catalytic activity
- Hyaluronan lyase was the first activity discovered for the family PL30 [2].
- First charge neutralizer identification
- To be determined
- First Brønstead acid and base residue identification
- To be determinated
- First 3-D structure
- To be determinated
References
- Drula E, Garron ML, Dogan S, Lombard V, Henrissat B, and Terrapon N. (2022). The carbohydrate-active enzyme database: functions and literature. Nucleic Acids Res. 2022;50(D1):D571-D577. DOI:10.1093/nar/gkab1045 |
- Helbert W, Poulet L, Drouillard S, Mathieu S, Loiodice M, Couturier M, Lombard V, Terrapon N, Turchetto J, Vincentelli R, and Henrissat B. (2019). Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space. Proc Natl Acad Sci U S A. 2019;116(13):6063-6068. DOI:10.1073/pnas.1815791116 |