Carbohydrate Binding Module Family 96

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• Author: Wenwen Tao
• Responsible Curator: Yaoguang Chang

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CAZy DB link

http://www.cazy.org/CBM96.html

Ligand specificities

Members of the CBM96 family are present within various CAZyme families, including PL8, PL35, GH16, and GH136, suggesting that they may possess a wide range of diverse activities. TM6-N4, originating from the marine thermophilic bacterium Defluviitalea phaphyphila, and DmCBM96-1/DmCBM96-2, derived from the bacterium Dysgonomonas sp. HDW5A isolated from Hydrophilus acuminatus , are currently the characterized members of the CBM96 family. TM6-N4 is associated with the catalytic module of PL39 alginate lyase, and its specific binding capability towards alginate was confirmed by affinity electrophoresis analysis. However, isothermal titration calorimetry analysis revealed that TM6-N4 exhibits no significant preference for mannuronate or guluronate oligosaccharides [1]. DmCBM96-1 and DmCBM96-2, which are consecutive CBMs linked to chondroitin sulfate (CS) lyase PL8_3, have been confirmed to bind to CS-A (chondroitin 4-sulfate), CS-C (chondroitin 6-sulfate), and Aj-fCS (consisting of CS-type backbone and sulfated fucose branches), but not to other polyuronic acids such as hyaluronic acid (HA), dermatan sulfate (DS), heparin (HS), pectin, and alginate. Comparatively, the relative affinity of both DmCBM96 proteins to CS-C are higher than CS-A [2].

Structural Features

Figure 1. Crystal structure of TM6-N4. The strand, helix, loop and Ca^{2+ were colored in cyan, yellow, white and green respectively.}

Upon BLASTP analysis, DmCBM96-1 exhibits a relatively low sequence identity of approximately 32% when compared to TM6-N4, whereas the sequence identity between DmCBM96-2 and TM6-N4 is even lower, at 25%. The crystal structures of TM6-N4 (1.35 Å) and DmCBM96-1 (2.20 Å) reveal that they both exhibit a typical β-sandwich fold, comprising two antiparallel β-sheets consisting of 11 β-strands, but differ in helixes. The binding sites of both DmCBM96-1 and TM6-N4 are located within the loop regions. Site-directed mutagenesis assay revealed that the basic amino acids Arg27, Lys45, Arg53, Arg157, and the aromatic amino acid Tyr51 at the binding site of DmCBM96-1 are crucial for CS binding [2]. While , the amino acids located at the bottom or wall of the shallow groove of TM6-N4, including Lys10, Lys22, Lys25, Lys27, Lys31, Arg36, and Tyr159, are essential for alginate binding [1]. Among these, the crucial amino acids Lys45 and Arg53 in DmCBM96-1 correspond to the key amino acids Lys27 and Arg36 in TM6-N4, respectively, and they are highly conserved within the CBM96 family, suggesting their pivotal roles in the ligand binding process.

Functionalities

Members of the CBM96 family are annotated by dbCAN to be associated with several CAZyme families, and their presumed physiological role is to facilitate enzyme-carbohydrate interactions and enhance the local concentration of enzymes near substrates, thereby promoting the degradation of polysaccharides. Based on the polysaccharide-binding specificity of the CBM96 family, it may also have potential applications in the study of cellular structures or the detection of pathogenic bacteria. Furthermore, through fusion expression, CBM96 could potentially be utilized to optimize the properties of other CAZymes [1].

Family Firsts

First Identified

The TM6-N4 derived from the alginate lyase Dp0100 of marine bacterium Defluviitalea phaphyphila, which was first reported in 2023, represents the first characterized member of the CBM96 family [1].

First Structural Characterization

Alginate-binding protein TM6-N4 is the first member of the CBM96 family to undergo crystal structure characterization (PDB 7vbo) [1]. Subsequently, CS-binding protein DmCBM96-1 was also characterized (PDB 2pqr) [2].

References

1. Ji S, Tian X, Li X, and She Q. (2023). Identification and structural analysis of a carbohydrate-binding module specific to alginate, a representative of a new family, CBM96. J Biol Chem. 2023;299(2):102854. DOI:10.1016/j.jbc.2022.102854 |
2. Liu G, Mei X, Zhang Y, Chen G, Li J, Tao W, Sun M, Zheng L, Chang Y, and Xue C. (2024). Characterization and Structural Analysis of a Novel Carbohydrate-Binding Module from Family 96 with Chondroitin Sulfate-Specific Binding Capacity. J Agric Food Chem. 2024;72(23):13196-13204. DOI:10.1021/acs.jafc.4c00090 |