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Difference between revisions of "Glycoside Hydrolase Family 193"
| Line 37: | Line 37: | ||
== Catalytic Residues == | == Catalytic Residues == | ||
| − | E246(SkSGL) is the putative general acid as this residue is structurally well-superimposed with the general acid (E262) in GH162 β-1,2-glucanase from Talaromyces funiculosus <cite>#Tanaka2019</cite> | + | E246(SkSGL) is the putative general acid as this residue is structurally well-superimposed with the general acid (E262) in [[GH162]] β-1,2-glucanase from <i>Talaromyces funiculosus</i> <cite>#Tanaka2019</cite>. <br> |
| + | D160(SkSGL) is one of the candidate for the general base as this residue is a spatially conserved residue shared with several β-1,2-glucanases; [[GH144]] (from <i>Chitinophaga pinensis</i> and <i>Xanthomonas campestris</i> pv. <i>campestris</i>) [[GH192]] (from <i>P. gaetbulicola</i> and <i>Endozoicomonas elysicola</i>), and [[GH194]] (from <i>P. gaetbulicala</i>) <cite>#Nakajima2025, #Abe2017</cite>. However, no complex structure with a substrate is available. | ||
== Three-dimensional structures == | == Three-dimensional structures == | ||
| − | + | Currently not determined. | |
== Family Firsts == | == Family Firsts == | ||
| − | ;First stereochemistry determination: | + | ;First stereochemistry determination: A bacterial β-1,2-glucanase from <i>S. kedieii</i> by monitoring the change in optical rotation <cite>#Nakajima2025</cite>. |
| − | ;First catalytic nucleophile identification: | + | ;First catalytic nucleophile identification: not known. |
| − | ;First general acid/base residue identification: | + | ;First general acid/base residue identification: not known. |
| − | ;First 3-D structure: | + | ;First 3-D structure: not determined. |
== References == | == References == | ||
| Line 52: | Line 53: | ||
#Nakajima2025 pmid=40411428 | #Nakajima2025 pmid=40411428 | ||
#Tanaka2019 pmid=30926603 | #Tanaka2019 pmid=30926603 | ||
| + | #Abe2017 pmid=28270506 | ||
</biblio> | </biblio> | ||
<!-- Do not delete this Category tag --> | <!-- Do not delete this Category tag --> | ||
[[Category:Glycoside Hydrolase Families|GH193]] | [[Category:Glycoside Hydrolase Families|GH193]] | ||
Revision as of 09:27, 25 February 2026
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
| Glycoside Hydrolase Family GH193 | |
| Clan | GH-S |
| Mechanism | inverting |
| Active site residues | not known |
| CAZy DB link | |
| https://www.cazy.org/GH193.html | |
Substrate specificities
SkSGL(Sked_30460, KEGG) from Sanguibacter kedieii is specific to β-1,2-glucan among polysaccharides. The enzyme hydrolyzes β-1,2-glucan endolytically to produce β-1,2-glucooligosaccharides [1].
Kinetics and Mechanism
SkSGL follows anomer-inverting mechanism, which is determined by measuring change in optical rotation during hydrolysis of β-1,2-glucan [1].
Catalytic Residues
E246(SkSGL) is the putative general acid as this residue is structurally well-superimposed with the general acid (E262) in GH162 β-1,2-glucanase from Talaromyces funiculosus [2].
D160(SkSGL) is one of the candidate for the general base as this residue is a spatially conserved residue shared with several β-1,2-glucanases; GH144 (from Chitinophaga pinensis and Xanthomonas campestris pv. campestris) GH192 (from P. gaetbulicola and Endozoicomonas elysicola), and GH194 (from P. gaetbulicala) [1, 3]. However, no complex structure with a substrate is available.
Three-dimensional structures
Currently not determined.
Family Firsts
- First stereochemistry determination
- A bacterial β-1,2-glucanase from S. kedieii by monitoring the change in optical rotation [1].
- First catalytic nucleophile identification
- not known.
- First general acid/base residue identification
- not known.
- First 3-D structure
- not determined.
References
- Nakajima M, Tanaka N, Motouchi S, Kobayashi K, Shimizu H, Abe K, Hosoyamada N, Abara N, Morimoto N, Hiramoto N, Nakata R, Takashima A, Hosoki M, Suzuki S, Shikano K, Fujimaru T, Imagawa S, Kawadai Y, Wang Z, Kitano Y, Nihira T, Nakai H, and Taguchi H. (2025). New glycoside hydrolase families of β-1,2-glucanases. Protein Sci. 2025;34(6):e70147. DOI:10.1002/pro.70147 |
- Tanaka N, Nakajima M, Narukawa-Nara M, Matsunaga H, Kamisuki S, Aramasa H, Takahashi Y, Sugimoto N, Abe K, Terada T, Miyanaga A, Yamashita T, Sugawara F, Kamakura T, Komba S, Nakai H, and Taguchi H. (2019). Identification, characterization, and structural analyses of a fungal endo-β-1,2-glucanase reveal a new glycoside hydrolase family. J Biol Chem. 2019;294(19):7942-7965. DOI:10.1074/jbc.RA118.007087 |
- Abe K, Nakajima M, Yamashita T, Matsunaga H, Kamisuki S, Nihira T, Takahashi Y, Sugimoto N, Miyanaga A, Nakai H, Arakawa T, Fushinobu S, and Taguchi H. (2017). Biochemical and structural analyses of a bacterial endo-β-1,2-glucanase reveal a new glycoside hydrolase family. J Biol Chem. 2017;292(18):7487-7506. DOI:10.1074/jbc.M116.762724 |