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Difference between revisions of "Glycoside Hydrolase Family 194"
| Line 32: | Line 32: | ||
== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
| − | + | PgSGL3 follows anomer-inverting mechanism, which is determined by measuring change in optical rotation during hydrolysis of β-1,2-glucan <cite>#Nakajima2025</cite>. | |
== Catalytic Residues == | == Catalytic Residues == | ||
Revision as of 09:43, 25 February 2026
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
| Glycoside Hydrolase Family GH194 | |
| Clan | GH-S |
| Mechanism | inverting |
| Active site residues | not known |
| CAZy DB link | |
| https://www.cazy.org/GH194.html | |
Substrate specificities
PgSGL3(H744_1c0222, KEGG) from Photobacterium gaetbulicola is specific to β-1,2-glucan among polysaccharides. The enzyme hydrolyzes β-1,2-glucan endolytically to produce β-1,2-glucooligosaccharides
Kinetics and Mechanism
PgSGL3 follows anomer-inverting mechanism, which is determined by measuring change in optical rotation during hydrolysis of β-1,2-glucan [1].
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Content is to be added here.
Family Firsts
- First stereochemistry determination
- Content is to be added here.
- First catalytic nucleophile identification
- Content is to be added here.
- First general acid/base residue identification
- Content is to be added here.
- First 3-D structure
- Content is to be added here.
References
- Nakajima M, Tanaka N, Motouchi S, Kobayashi K, Shimizu H, Abe K, Hosoyamada N, Abara N, Morimoto N, Hiramoto N, Nakata R, Takashima A, Hosoki M, Suzuki S, Shikano K, Fujimaru T, Imagawa S, Kawadai Y, Wang Z, Kitano Y, Nihira T, Nakai H, and Taguchi H. (2025). New glycoside hydrolase families of β-1,2-glucanases. Protein Sci. 2025;34(6):e70147. DOI:10.1002/pro.70147 |
- Tanaka N, Nakajima M, Narukawa-Nara M, Matsunaga H, Kamisuki S, Aramasa H, Takahashi Y, Sugimoto N, Abe K, Terada T, Miyanaga A, Yamashita T, Sugawara F, Kamakura T, Komba S, Nakai H, and Taguchi H. (2019). Identification, characterization, and structural analyses of a fungal endo-β-1,2-glucanase reveal a new glycoside hydrolase family. J Biol Chem. 2019;294(19):7942-7965. DOI:10.1074/jbc.RA118.007087 |
- Lo Conte C, Allegrini C, Matucci A, Bartolucci M, Rosi E, Camiciottoli G, Amendola M, Pistolesi M, and Bargagli E. (2018). Immunoglobulin replacement therapy for yellow nail syndrome. Scand J Immunol. 2018;87(3). DOI:10.1111/sji.12639 |