Glycoside Hydrolase Family 42

This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.

• Author: ^^^Marco Moracci^^^
• Responsible Curator: ^^^Marco Moracci^^^

---

Glycoside Hydrolase Family GH42
Clan	GH-A
Mechanism	retaining
Active site residues	known
CAZy DB link
http://www.cazy.org/fam/GH42.html

Substrate specificities

Content is to be added here.

This is an example of how to make references to a journal article [1]. (See the References section below). Multiple references can go in the same place like this [2, 3]. You can even cite books using just the ISBN [4]. References that are not in PubMed can be typed in by hand [5].

Kinetics and Mechanism

Content is to be added here.

Catalytic Residues

Content is to be added here.

Three-dimensional structures

Content is to be added here.

Family Firsts

First sterochemistry determination

Cite some reference here, with a short (1-2 sentence) explanation [2].

First catalytic nucleophile identification

Cite some reference here, with a short (1-2 sentence) explanation [5].

First general acid/base residue identification

Cite some reference here, with a short (1-2 sentence) explanation [3].

First 3-D structure

Cite some reference here, with a short (1-2 sentence) explanation [4].

References

1. Kosugi A, Murashima K, and Doi RH. (2002). Characterization of two noncellulosomal subunits, ArfA and BgaA, from Clostridium cellulovorans that cooperate with the cellulosome in plant cell wall degradation. J Bacteriol. 2002;184(24):6859-65. DOI:10.1128/JB.184.24.6859-6865.2002 |

6. =18068682

7. Ohtsu N, Motoshima H, Goto K, Tsukasaki F, and Matsuzawa H. (1998). Thermostable beta-galactosidase from an extreme thermophile, Thermus sp. A4: enzyme purification and characterization, and gene cloning and sequencing. Biosci Biotechnol Biochem. 1998;62(8):1539-45. DOI:10.1271/bbb.62.1539 |
8. Kang SK, Cho KK, Ahn JK, Bok JD, Kang SH, Woo JH, Lee HG, You SK, and Choi YJ. (2005). Three forms of thermostable lactose-hydrolase from Thermus sp. IB-21: cloning, expression, and enzyme characterization. J Biotechnol. 2005;116(4):337-46. DOI:10.1016/j.jbiotec.2004.07.019 |
9. Yuan T, Yang P, Wang Y, Meng K, Luo H, Zhang W, Wu N, Fan Y, and Yao B. (2008). Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius. Biotechnol Lett. 2008;30(2):343-8. DOI:10.1007/s10529-007-9551-y |
10. Møller PL, Jørgensen F, Hansen OC, Madsen SM, and Stougaard P. (2001). Intra- and extracellular beta-galactosidases from Bifidobacterium bifidum and B. infantis: molecular cloning, heterologous expression, and comparative characterization. Appl Environ Microbiol. 2001;67(5):2276-83. DOI:10.1128/AEM.67.5.2276-2283.2001 |
11. Van Laere KM, Abee T, Schols HA, Beldman G, and Voragen AG. (2000). Characterization of a novel beta-galactosidase from Bifidobacterium adolescentis DSM 20083 active towards transgalactooligosaccharides. Appl Environ Microbiol. 2000;66(4):1379-84. DOI:10.1128/AEM.66.4.1379-1384.2000 |
12. Hinz SW, van den Brock LA, Beldman G, Vincken JP, and Voragen AG. (2004). beta-galactosidase from Bifidobacterium adolescentis DSM20083 prefers beta(1,4)-galactosides over lactose. Appl Microbiol Biotechnol. 2004;66(3):276-84. DOI:10.1007/s00253-004-1745-9 |

[[Category:Glycoside Hydrolase Families|GHnnn]]