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Difference between revisions of "Glycoside Hydrolase Family 12"
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== Substrate specificities == | == Substrate specificities == | ||
| − | + | The substrate specificities found among the [[glycoside hydrolases]] of family 12 are: ''[[endo]]''-β-1,4-glucanase (EC 3.2.1.4), ''xyloglucan hydrolase (EC 3.2.1.151), ''[[endo]]''-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan ''[[endo]]''-transglycosylase (EC 2.4.1.207),. | |
| − | + | ||
| + | References that are not in PubMed can be typed in by hand <cite>MikesClassic</cite>. | ||
Revision as of 03:17, 5 March 2010
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author: ^^^Mats Sandgren^^^
- Responsible Curator: ^^^Mats Sandgren^^^
| Glycoside Hydrolase Family GHnn | |
| Clan | GH-C |
| Mechanism | retaining |
| Active site residues | known |
| CAZy DB link | |
| http://www.cazy.org/fam/GH12.html | |
Substrate specificities
The substrate specificities found among the glycoside hydrolases of family 12 are: endo-β-1,4-glucanase (EC 3.2.1.4), xyloglucan hydrolase (EC 3.2.1.151), endo-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan endo-transglycosylase (EC 2.4.1.207),.
References that are not in PubMed can be typed in by hand [1].
Kinetics and Mechanism
Content is to be added here.
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Content is to be added here.
Family Firsts
- First sterochemistry determination
- Cite some reference here, with a short (1-2 sentence) explanation [2].
- First catalytic nucleophile identification
- Cite some reference here, with a short (1-2 sentence) explanation [1].
- First general acid/base residue identification
- Cite some reference here, with a short (1-2 sentence) explanation [3].
- First 3-D structure
- Cite some reference here, with a short (1-2 sentence) explanation [4].
References
-
Sinnott, M.L. (1990) Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90, 1171-1202. DOI: 10.1021/cr00105a006
- Comfort DA, Bobrov KS, Ivanen DR, Shabalin KA, Harris JM, Kulminskaya AA, Brumer H, and Kelly RM. (2007). Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Biochemistry. 2007;46(11):3319-30. DOI:10.1021/bi061521n |
- He S and Withers SG. (1997). Assignment of sweet almond beta-glucosidase as a family 1 glycosidase and identification of its active site nucleophile. J Biol Chem. 1997;272(40):24864-7. DOI:10.1074/jbc.272.40.24864 |