Glycoside Hydrolase Family 14

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Undergraduate students, (post)graduate students, post-doctoral researchers, research associates, and professors are all welcomed to contribute!

• If you are interested in contributing to CAZypedia, please see the 10th anniversary publication [1] and this page for an overview of the content and mission of our community-driven resource. Future authors of CAZypedia can include the CAZypedia article [1] and cite individual pages in their curricula vitae to reflect their contribution.
• Until this CAZypedia page is completed, current information on the composition of this family is available via the corresponding page of the CAZy database [2]:
  https://www.cazy.org/GH14.html

References

1. CAZypedia Consortium (2018). Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes. Glycobiology. 2018;28(1):3-8. DOI:10.1093/glycob/cwx089 |
2. Drula E, Garron ML, Dogan S, Lombard V, Henrissat B, and Terrapon N. (2022). The carbohydrate-active enzyme database: functions and literature. Nucleic Acids Res. 2022;50(D1):D571-D577. DOI:10.1093/nar/gkab1045 |

• Author: ^^^Bunzo Mikami^^^
• Responsible Curator: ^^^Kousaku Murata^^^

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Glycoside Hydrolase Family GHnn
Clan	GH-x
Mechanism	retaining/inverting
Active site residues	known/not known
CAZy DB link
http://www.cazy.org/fam/GHnn.html

Substrate specificities

Content is to be added here.

This is an example of how to make references to a journal article [3]. (See the References section below). Multiple references can go in the same place like this [3, 4]. You can even cite books using just the ISBN [5]. References that are not in PubMed can be typed in by hand [6].

Kinetics and Mechanism

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Catalytic Residues

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Three-dimensional structures

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Family Firsts

First stereochemistry determination

Cite some reference here, with a short (1-2 sentence) explanation [3].

First catalytic nucleophile identification

Cite some reference here, with a short (1-2 sentence) explanation [6].

First general acid/base residue identification

Cite some reference here, with a short (1-2 sentence) explanation [4].

First 3-D structure

Cite some reference here, with a short (1-2 sentence) explanation [5].

References

3. Comfort DA, Bobrov KS, Ivanen DR, Shabalin KA, Harris JM, Kulminskaya AA, Brumer H, and Kelly RM. (2007). Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Biochemistry. 2007;46(11):3319-30. DOI:10.1021/bi061521n |
4. He S and Withers SG. (1997). Assignment of sweet almond beta-glucosidase as a family 1 glycosidase and identification of its active site nucleophile. J Biol Chem. 1997;272(40):24864-7. DOI:10.1074/jbc.272.40.24864 |

6. Sinnott, M.L. (1990) Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90, 1171-1202. DOI: 10.1021/cr00105a006