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Difference between revisions of "Carbohydrate Binding Module Family 76"
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| − | * [[Author]]: | + | * [[Author]]: [[User:Ana Luis|Ana Luis]] |
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== Ligand specificities == | == Ligand specificities == | ||
| − | CBM76 is a small family containing only five members found exclusively in Ruminococci. The founding member of this family is CBM76<sub>RfGH44</sub> from ''Ruminococcus flavefaciens'' that recognizes different β-1,4-glucans <cite>Venditto2016</cite>. By isothermal titration calorimetry, the affinity of this binding module to xyloglucan (K<sub>A</sub> 1.1 x 10<sup>6</sup>) is 100-fold higher than to glucomannan, barley β-glucan, hydroxyethylcellulose and XXXG (the repeating unit of xyloglucan, where X comprises glucose decorated at O6 with xylose and G corresponds to undecorated glucose) <cite>Venditto2016</cite>. | + | CBM76 is a small family containing only five members found exclusively in Ruminococci. The founding member of this family is CBM76<sub>RfGH44</sub> from ''Ruminococcus flavefaciens'' that recognizes different β-1,4-glucans <cite>Venditto2016</cite>. By isothermal titration calorimetry, the affinity of this binding module to xyloglucan (K<sub>A</sub> 1.1 x 10<sup>6</sup> M<sup>-1</sup>) is 100-fold higher than to glucomannan, barley β-glucan, hydroxyethylcellulose and XXXG (the repeating unit of xyloglucan, where X comprises glucose decorated at O6 with xylose and G corresponds to undecorated glucose) <cite>Venditto2016</cite>. |
== Structural Features == | == Structural Features == | ||
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== Functionalities == | == Functionalities == | ||
| − | The founding member of this family, CBM76<sub>RfGH44</sub>, is appended to a glycoside hydrolase family 44 ([[GH44]]) and a C-terminal dockerin. The binding module specificity | + | The founding member of this family, CBM76<sub>RfGH44</sub>, is appended to a glycoside hydrolase family 44 ([[GH44]]) and a C-terminal dockerin. The carbohydrate-binding module specificity for β-1,4-glucans is consistent with the previously reported activity for [[GH44]] enzymes (endoglucanase or xyloglucanase) <cite>Venditto2016</cite>; however, the impact of CBM76<sub>RfGH44</sub> binding module on [[GH44]] substrate recognition and catalysis it is not known. |
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== Family Firsts == | == Family Firsts == | ||
| − | ;First Identified:CBM76<sub>RfGH44</sub> from ''Ruminococcus flavefaciens'' <cite>Venditto2016</cite>. | + | ;First Identified: CBM76<sub>RfGH44</sub> from ''Ruminococcus flavefaciens'' <cite>Venditto2016</cite>. |
;First Structural Characterization: No three-dimensional structure has been solved for this CBM family. | ;First Structural Characterization: No three-dimensional structure has been solved for this CBM family. | ||
Latest revision as of 13:19, 18 December 2021
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Ligand specificities
CBM76 is a small family containing only five members found exclusively in Ruminococci. The founding member of this family is CBM76RfGH44 from Ruminococcus flavefaciens that recognizes different β-1,4-glucans [1]. By isothermal titration calorimetry, the affinity of this binding module to xyloglucan (KA 1.1 x 106 M-1) is 100-fold higher than to glucomannan, barley β-glucan, hydroxyethylcellulose and XXXG (the repeating unit of xyloglucan, where X comprises glucose decorated at O6 with xylose and G corresponds to undecorated glucose) [1].
Structural Features
No three-dimensional structure has been solved for this CBM family.
Functionalities
The founding member of this family, CBM76RfGH44, is appended to a glycoside hydrolase family 44 (GH44) and a C-terminal dockerin. The carbohydrate-binding module specificity for β-1,4-glucans is consistent with the previously reported activity for GH44 enzymes (endoglucanase or xyloglucanase) [1]; however, the impact of CBM76RfGH44 binding module on GH44 substrate recognition and catalysis it is not known.
Family Firsts
- First Identified
- CBM76RfGH44 from Ruminococcus flavefaciens [1].
- First Structural Characterization
- No three-dimensional structure has been solved for this CBM family.
References
- Venditto I, Luis AS, Rydahl M, Schückel J, Fernandes VO, Vidal-Melgosa S, Bule P, Goyal A, Pires VM, Dourado CG, Ferreira LM, Coutinho PM, Henrissat B, Knox JP, Baslé A, Najmudin S, Gilbert HJ, Willats WG, and Fontes CM. (2016). Complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition. Proc Natl Acad Sci U S A. 2016;113(26):7136-41. DOI:10.1073/pnas.1601558113 |