Difference between revisions of "Carbohydrate Binding Module Family 82"

Latest revision as of 13:14, 18 December 2021

This page has been approved by the Responsible Curator as essentially complete. CAZypedia is a living document, so further improvement of this page is still possible. If you would like to suggest an addition or correction, please contact the page's Responsible Curator directly by e-mail.

CAZy DB link
https://www.cazy.org/CBM82.html

Ligand specificities

Figure 1. Domain architecture of E. rectale Amy13K The CBM and GH families are noted. The 'S' indicate the signal sequence and the 'anchor' is a cell wall anchor region. The unknown domain has no known function, however, deletion eliminates enzyme activity [].

The founding member of this family and the first module to be characterized is the first CBM found in the cell-wall anchored GH13 enzyme Amy13K from Eubacterium rectale (see Fig1). It was found to bind beta-cyclodextrin and glycogen with similar affinity, with slightly weaker affinity for maltoheptaose as determined by isothermal titration calorimetry. The module was also found to bind to corn starch granules, both from a wild-type source and from a high amylose source (HiMaize 260) with approximately equal affinity but did not demonstrate binding to potato starch or a chemically crosslinked starch (Fibersym) as determined via depletion assays. Binding to amylopectin and pullulan was also demonstrated via affinity electrophoresis. [1].

Structural Features

Secondary structure analysis and alignments suggest they are likely to be beta-sandwich type folds similar to the CBM41 family [1].

Functionalities

This family has been found to be exclusively associated with GH13 family amylases from a quite narrow taxonomic range within Roseburia and Eubacterium rectale [1]. Removal of the only CBM82 from the E. rectale Amy13K enzyme resulted in an approximately 2-fold decrease in activity of the enzyme towards amylopectin or potato starch, but resulted in a larger 5-fold decrease in the activity of the enzyme towards corn starch granules, suggesting an important role in targeting the enzyme to this substrate. [1]

Family Firsts

First Identified: In Roseburia inulinivorans as a predicted CBM [2]

First Characterized: From E. rectale Amy13K, establishing the family [1]

References

All Medline abstracts: PubMed

@@ Line 2: / Line 2: @@
 <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption -->
 {{CuratorApproved}}
-* [[Author]]: ^^^Darrell Cockburn^^^
+* [[Author]]: [[User:Darrell Cockburn|Darrell Cockburn]]
-* [[Responsible Curator]]:  ^^^Nicole Koropatkin^^^
+* [[Responsible Curator]]:  [[User:Nicole Koropatkin|Nicole Koropatkin]]
 ----
@@ Line 20: / Line 20: @@
 [[File:Amy13K_CBM82_highlight.jpg|thumb|600px|right|'''Figure 1. Domain architecture of ''E. rectale'' Amy13K''' The CBM and GH families are noted. The 'S' indicate the signal sequence and the 'anchor' is a cell wall anchor region. The unknown domain has no known function, however, deletion eliminates enzyme activity <cite>Cockburn2018</cite>.]]
-The founding member of this family and the first module to be characterized is the first CBM found in the cell-wall anchored Amy13K from ''Eubacterium rectale'' (see Fig1). It was found to bind beta-cyclodextrin and glycogen with similar affinity, with slightly weaker affinity for maltoheptaose as determined by isothermal titration calorimetry. The module was also found to bind to corn starch granules, both from a wild-type source and from a high amylose source (HiMaize 260) with approximately equal affinity but did not demonstrate binding to potato starch or a chemically crosslinked starch (Fibersym) as determined via depletion assays. Binding to amylopectin and pullulan was also demonstrated via affinity electrophoresis.
+The founding member of this family and the first module to be characterized is the first CBM found in the cell-wall anchored [[GH13]] enzyme Amy13K from ''Eubacterium rectale'' (see Fig1). It was found to bind beta-cyclodextrin and glycogen with similar affinity, with slightly weaker affinity for maltoheptaose as determined by isothermal titration calorimetry. The module was also found to bind to corn starch granules, both from a wild-type source and from a high amylose source (HiMaize 260) with approximately equal affinity but did not demonstrate binding to potato starch or a chemically crosslinked starch (Fibersym) as determined via depletion assays. Binding to amylopectin and pullulan was also demonstrated via affinity electrophoresis.
 <cite>Cockburn2018</cite>.