User:Wade Abbott

Dr. Abbott received his PhD from the University of Victoria in 2005. He then studied the molecular basis of protein-carbohydrate interactions under Dr. ^^^Alisdair Boraston^^^ at the University of Victoria. In 2008, Dr. Abbott joined Dr. ^^^Harry Gilbert^^^ at the Complex Carbohydrate Research Centre, at the University of Georgia where he investigated the functional genomics of carbohydrate utilization pathways from intestinal bacteria. Currently, Dr. Abbott is a Research Scientist for Agriculture and Agri-Food Canada based at the Lethbridge Research Centre and Adjunct Professor at the University of Lethbridge. His research program investigates the mechanisms of complex carbohydrate modification by intestinal bacteria, and is developing applications for carbohydrates in animal agriculture and human intestinal health.

I have contributed to structure-function studies of the following CAZyme Families:

Glycoside Hydrolases

• GH20 Streptococcus pneumoniae exo-β-D-N-acetylglucosaminidase (SpnGH20AB/StrH) [1, 2].

• GH28 Yersinia enterocolitica exopolygalacturonase (YeGH28) [3]. *Family First

• GH85 Streptococcus pneumoniae endo-β-D-glucosaminidase D (SpGH85/EndoD) [4]. *Family First

Polysaccharide Lyases

• PL2 Yersinia enterocolitica perplasmic pectate lyase (YePL2A) [5]. Paenibacillus sp. Y412MC10 Cytoplasmic endolytic pectate lyase (PaePL2) [6]. *Family First

• PL22 Yersinia enterocolitica Cytoplasmic oligogalacturonate lyase (YePL22/Ogl) [7]. *Family First

Carbohydrate Binding Modules

• CBM6 [8],[9]
• CBM32 Yersinia enterocolitica polygalacturonic acid binding protein (YeCBM32) [10]. Streptococcus pneumoniae EndoD CBM (SpnCBM32) [11].
• CBM35 [12]
• CBM51 Clostridium perfringens blood group binding CBMs (GH95CBM51 and GH98CBM51) [13]

Carbohydrate Esterases

• CE8 Yersinia enterocolitica pectin methylesterase [14]

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References

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3. Abbott DW and Boraston AB. (2007). The structural basis for exopolygalacturonase activity in a family 28 glycoside hydrolase. J Mol Biol. 2007;368(5):1215-22. DOI:10.1016/j.jmb.2007.02.083 |
4. Abbott DW, Macauley MS, Vocadlo DJ, and Boraston AB. (2009). Streptococcus pneumoniae endohexosaminidase D, structural and mechanistic insight into substrate-assisted catalysis in family 85 glycoside hydrolases. J Biol Chem. 2009;284(17):11676-89. DOI:10.1074/jbc.M809663200 |
5. Abbott DW and Boraston AB. (2007). A family 2 pectate lyase displays a rare fold and transition metal-assisted beta-elimination. J Biol Chem. 2007;282(48):35328-36. DOI:10.1074/jbc.M705511200 |
6. Abbott DW, Thomas D, Pluvinage B, and Boraston AB. (2013). An ancestral member of the polysaccharide lyase family 2 displays endolytic activity and magnesium dependence. Appl Biochem Biotechnol. 2013;171(7):1911-23. DOI:10.1007/s12010-013-0483-9 |
8. Abbott DW, Macauley MS, Vocadlo DJ, and Boraston AB. (2009). Streptococcus pneumoniae endohexosaminidase D, structural and mechanistic insight into substrate-assisted catalysis in family 85 glycoside hydrolases. J Biol Chem. 2009;284(17):11676-89. DOI:10.1074/jbc.M809663200 |
10. Abbott DW, Hrynuik S, and Boraston AB. (2007). Identification and characterization of a novel periplasmic polygalacturonic acid binding protein from Yersinia enterolitica. J Mol Biol. 2007;367(4):1023-33. DOI:10.1016/j.jmb.2007.01.030 |