CAZypedia needs your help!
We have many unassigned pages in need of Authors and Responsible Curators. See a page that's out-of-date and just needs a touch-up? - You are also welcome to become a CAZypedian. Here's how.
Scientists at all career stages, including students, are welcome to contribute.
Learn more about CAZypedia's misson here and in this article.
Totally new to the CAZy classification? Read this first.
Difference between revisions of "Glycoside Hydrolase Family 114"
| Line 1: | Line 1: | ||
<!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption --> | <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption --> | ||
| − | <includeonly>{{ | + | <includeonly>{{CuratorApproved}}</includeonly> |
* [[Author]]: ^^^Spencer Williams^^^ | * [[Author]]: ^^^Spencer Williams^^^ | ||
* [[Responsible Curator]]: ^^^Spencer Williams^^^ | * [[Responsible Curator]]: ^^^Spencer Williams^^^ | ||
| Line 29: | Line 29: | ||
== Substrate specificities == | == Substrate specificities == | ||
| − | Only a single enzyme of [[glycoside hydrolase]] family 114 has been characterized; an ''endo''-α-1,4-polygalactosaminidase from ''Pseudomonas'' sp. 881 <cite>Tamura1995</cite>. This enzyme hydrolyzes α-1,4-polygalactosamine to oligosaccharides in an [[endo]]-acting manner. α-1,4-Polygalactosamine, also known as galactosaminoglycan, is a polymer consisting of α-1,4-linked galactosamine residues, which is only partially ''N''-acetylated, and may also contain ''N''-formyl residues, and is produced by fungi including ''Aspergillus parasiticus'' and ''Paecilomyces'' sp. I-1. An endogalactosaminidase has been purified from ''Streptomyces griseus''; the sequence of this protein is unknown <cite>Riessig1975</cite>. | + | Only a single enzyme of [[glycoside hydrolase]] family 114 has been characterized; an ''endo''-α-1,4-polygalactosaminidase from ''Pseudomonas'' sp. 881 <cite>Tamura1995</cite>. This enzyme hydrolyzes α-1,4-polygalactosamine to oligosaccharides in an [[endo]]-acting manner. α-1,4-Polygalactosamine, also known as galactosaminoglycan, is a polymer consisting of α-1,4-linked galactosamine residues, which is only partially ''N''-acetylated, and may also contain ''N''-formyl residues, and is produced by fungi including ''Aspergillus parasiticus'' <cite>Distiller1960</cite> and ''Paecilomyces'' sp. I-1 <cite>Takagi1985</cite>. An endogalactosaminidase has been purified from ''Streptomyces griseus''; the sequence of this protein is unknown <cite>Riessig1975</cite>. |
== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
| Line 52: | Line 52: | ||
#Riessig1975 pmid=3271 | #Riessig1975 pmid=3271 | ||
#Tamura1988 Tamura, J.-I., Takagi, H., Kadowaki, K. Purification and Some Properties of the Endo α-1,4 Polygalactosaminidase from Pseudomonas sp., ''Agric. Biol. Chem.'' 1988, 52 , 2475-2484. [http://dx.doi.org/10.1080/00021369.1988.10869068]. | #Tamura1988 Tamura, J.-I., Takagi, H., Kadowaki, K. Purification and Some Properties of the Endo α-1,4 Polygalactosaminidase from Pseudomonas sp., ''Agric. Biol. Chem.'' 1988, 52 , 2475-2484. [http://dx.doi.org/10.1080/00021369.1988.10869068]. | ||
| − | + | #Distiller1960 pmid=13816939 | |
| + | #Takagi1985 Takagi, H., Kadowaki, K. Purification and Chemical Properties of a Flocculant Produced by Paecilomyces. ''Agric. Biol. Chem.'' 1985, 49, 3159-3164. [http://dx.doi.org/10.1080/00021369.1985.10867250] | ||
</biblio> | </biblio> | ||
[[Category:Glycoside Hydrolase Families|GH114]] | [[Category:Glycoside Hydrolase Families|GH114]] | ||
Revision as of 01:59, 3 September 2016
- Author: ^^^Spencer Williams^^^
- Responsible Curator: ^^^Spencer Williams^^^
| Glycoside Hydrolase Family GH114 | |
| Clan | none |
| Mechanism | probably retaining |
| Active site residues | not known |
| CAZy DB link | |
| https://www.cazy.org/GH114.html | |
Substrate specificities
Only a single enzyme of glycoside hydrolase family 114 has been characterized; an endo-α-1,4-polygalactosaminidase from Pseudomonas sp. 881 [1]. This enzyme hydrolyzes α-1,4-polygalactosamine to oligosaccharides in an endo-acting manner. α-1,4-Polygalactosamine, also known as galactosaminoglycan, is a polymer consisting of α-1,4-linked galactosamine residues, which is only partially N-acetylated, and may also contain N-formyl residues, and is produced by fungi including Aspergillus parasiticus [2] and Paecilomyces sp. I-1 [3]. An endogalactosaminidase has been purified from Streptomyces griseus; the sequence of this protein is unknown [4].
Kinetics and Mechanism
The endo-α-1,4-polygalactosaminidase from Pseudomonas sp. 881 possesses activity on deacetylated α-1,4-polygalactosamine, but no activity on fully N-acetylated α-1,4-polygalactosamine [5]. Tetraose and longer galactosamine oligosaccharides were hydrolyzed to galactosaminobiose and galactosaminotriose as the final products [6]. Based on the dependence of rate on the chain length of the substrate, it was proposed that the enzyme has 8 subsites [6] The enzyme is inhibited by metal ions including Hg2+, Fe2+ and Sn2+ [5].
Catalytic Residues
None known.
Three-dimensional structures
No 3-D structure has been reported for any member of this family.
Family Firsts
- First stereochemistry determination
- Not known, but a retaining mechanism may be inferred from report of transglycosylation activity [6].
- First catalytic nucleophile identification
- Not known.
- First general acid/base residue identification
- Not known.
- First 3-D structure
- None reported.
References
-
Tamura, J.-I., Hasegawa, K., Kadowaki, K., Igarashi, Y., Kodama, T. Molecular Cloning and Sequence Analysis of the Gene Encoding an Endo a-l,4 Polygalactosaminidase of Pseudomonas sp. 881. J. Fermentation Bioengineer., 1995, 80, 305. [1].
- DISTLER JJ and ROSEMAN S. (1960). Galactosamine polymers produced by Aspergillus parasiticus. J Biol Chem. 1960;235:2538-41. | Google Books | Open Library
-
Takagi, H., Kadowaki, K. Purification and Chemical Properties of a Flocculant Produced by Paecilomyces. Agric. Biol. Chem. 1985, 49, 3159-3164. [1]
- Reissig JL, Lai WH, and Glasgow JE. (1975). An endogalactosaminidase from Streptomyces griseus. Can J Biochem. 1975;53(12):1237-49. DOI:10.1139/o75-169 |
-
Tamura, J.-I., Takagi, H., Kadowaki, K. Purification and Some Properties of the Endo α-1,4 Polygalactosaminidase from Pseudomonas sp., Agric. Biol. Chem. 1988, 52 , 2475-2484. [1].
- Tamura J, Abe T, Hasegawa K, and Kadowaki K. (1992). The Mode of Action of Endo α-1,4 Polygalactosaminidase from Pseudomonas sp. 881 on Galactosaminooligosaccharides. Biosci Biotechnol Biochem. 1992;56(3):380-3. DOI:10.1271/bbb.56.380 |