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Difference between revisions of "Glycoside Hydrolase Family 147"
| Line 29: | Line 29: | ||
== Substrate specificities == | == Substrate specificities == | ||
| − | The founding member of GH147, BACOVA_05493 a β1,4-galactosidase demonstrates a preference towards longer oligosaccharides releasing | + | The founding member of GH147, BACOVA_05493 from ''Bacteroides ovatus'' is a β1,4-galactosidase. The enzyme demonstrates a preference towards longer oligosaccharides and β1,4-galactan, releasing galactopyranose from the oligosaccharide or polysaccharide chain. BACOVA_05493 is unable to hydrolyse galactobiose <cite>Luis2017</cite>. |
== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
| − | NMR analysis of the | + | NMR analysis of the galactose product released from galactotriose revealed a retaining mechanism of action <cite>Luis2017</cite>. |
== Catalytic Residues == | == Catalytic Residues == | ||
| − | + | ||
| + | Hydrophobic cluster analysis showed that BACOVA_05493 is a member of Clan GH-A. Based on this assumption the catalytic nucleophile and general acid/base residues of the founding member of GH147 were proposed as Glu300 and Glu203, respectively. This hypothesis was supported by mutagenesis data showing that the E300A and E203A mutants were catalytically inactive <cite>Luis2017</cite>. | ||
== Three-dimensional structures == | == Three-dimensional structures == | ||
| − | Currently there is no crystal structure of any member of GH147. | + | |
| + | Currently there is no crystal structure of any member of GH147, although sequence analysis and hydrophobic cluster analysis predicts a fold of an (α/β)<sub>8</sub> barrel. | ||
== Family Firsts == | == Family Firsts == | ||
| − | ;First stereochemistry determination: | + | ;First stereochemistry determination: BACOVA_05493 from ''B. ovatus'' was shown to have a retaining mechanism <cite>Luis2017</cite>. |
| − | ;First catalytic nucleophile identification: | + | ;First catalytic nucleophile identification: BACOVA_05493 from ''B. ovatus'' <cite>Luis2017</cite>. |
| − | ;First general acid/base residue identification: | + | ;First general acid/base residue identification: BACOVA_05493 from ''B. ovatus'' <cite>Luis2017</cite>. |
| − | ;First 3-D structure: Currently | + | |
| + | ;First 3-D structure: Currently no experimental structure is available, although BACOVA_05493 is predicted to fold into a (α/β)<sub>8</sub> barrel <cite>Luis2017</cite>. | ||
== References == | == References == | ||
Revision as of 10:50, 15 February 2018
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author: ^^^Jonathon Briggs^^^
- Responsible Curator: ^^^Harry Gilbert^^^
| Glycoside Hydrolase Family GH147 | |
| Clan | GH-A |
| Mechanism | retaining |
| Active site residues | known |
| CAZy DB link | |
| https://www.cazy.org/GH147.html | |
Substrate specificities
The founding member of GH147, BACOVA_05493 from Bacteroides ovatus is a β1,4-galactosidase. The enzyme demonstrates a preference towards longer oligosaccharides and β1,4-galactan, releasing galactopyranose from the oligosaccharide or polysaccharide chain. BACOVA_05493 is unable to hydrolyse galactobiose [1].
Kinetics and Mechanism
NMR analysis of the galactose product released from galactotriose revealed a retaining mechanism of action [1].
Catalytic Residues
Hydrophobic cluster analysis showed that BACOVA_05493 is a member of Clan GH-A. Based on this assumption the catalytic nucleophile and general acid/base residues of the founding member of GH147 were proposed as Glu300 and Glu203, respectively. This hypothesis was supported by mutagenesis data showing that the E300A and E203A mutants were catalytically inactive [1].
Three-dimensional structures
Currently there is no crystal structure of any member of GH147, although sequence analysis and hydrophobic cluster analysis predicts a fold of an (α/β)8 barrel.
Family Firsts
- First stereochemistry determination
- BACOVA_05493 from B. ovatus was shown to have a retaining mechanism [1].
- First catalytic nucleophile identification
- BACOVA_05493 from B. ovatus [1].
- First general acid/base residue identification
- BACOVA_05493 from B. ovatus [1].
- First 3-D structure
- Currently no experimental structure is available, although BACOVA_05493 is predicted to fold into a (α/β)8 barrel [1].
References
- Luis AS, Briggs J, Zhang X, Farnell B, Ndeh D, Labourel A, Baslé A, Cartmell A, Terrapon N, Stott K, Lowe EC, McLean R, Shearer K, Schückel J, Venditto I, Ralet MC, Henrissat B, Martens EC, Mosimann SC, Abbott DW, and Gilbert HJ. (2018). Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018;3(2):210-219. DOI:10.1038/s41564-017-0079-1 |