Carbohydrate Binding Module Family 55

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• Author: ^^^John Samuelson^^^
• Responsible Curator: ^^^Elizabeth Ficko-Blean^^^

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CAZy DB link

https://www.cazy.org/CBM55.html

Ligand specificities

A CBM55, which is ~60-aa long and contains eight cysteines in conserved positions, was first identified at the N-terminus of the GH18 chitinase of Entamoeba histolytica, the protist that causes dysentery and liver abscess (Fig. 1) [1, 2]. CBM55 members are also present at the N-termini of Jessie 3 lectins, which contain a self-aggregating (daub) domain, while CBM55 is the only domain in Jessie 1 and Jessie 2 lectins [3]. CBM55 members are present in GH18 chitinases and Jessie lectins of all five Entamoeba species that have been sequenced. CBM55 members are absent from other eukaryotes, eubacteria, and archaea, and so the CBM55 motif appears to have been “created from scratch” by the common ancestor to Entamoebae. CBM55 members of E. histolytica chitinase, Jessie 1, and Jessie 3 lectins, each produced by gene expression under a constitutive actin promoter in transformed trophozoites, demonstrated binding to chitin beads [1].

Structural Features

Content in this section should include, in paragraph form, a description of:

• Fold: Structural fold (beta trefoil, beta sandwich, etc.)
• Type: Include here Type A, B, or C and properties
• Features of ligand binding: Describe CBM binding pocket location (Side or apex) important residues for binding (W, Y, F, subsites), interact with reducing end, non-reducing end, planar surface or within polysaccharide chains. Include examples pdb codes. Metal ion dependent. Etc.

Functionalities

Content in this section should include, in paragraph form, a description of:

• Functional role of CBM: Describe common functional roles such as targeting, disruptive, anchoring, proximity/position on substrate.
• Most Common Associated Modules: 1. Glycoside Hydrolase Activity; 2. Additional Associated Modules (other CBM, FNIII, cohesin, dockerins, expansins, etc.)
• Novel Applications: Include here if CBM has been used to modify another enzyme, or if a CBM was used to label plant/mammalian tissues? Etc.

Family Firsts

First Identified

A CBM55 was first identified at the N-terminus of the GH18 chitinase of Entamoeba histolytica[1, 2].

First Structural Characterization

Insert archetype here, possibly including very brief synopsis.

References

1. Van Dellen K, Ghosh SK, Robbins PW, Loftus B, and Samuelson J. (2002). Entamoeba histolytica lectins contain unique 6-Cys or 8-Cys chitin-binding domains. Infect Immun. 2002;70(6):3259-63. DOI:10.1128/IAI.70.6.3259-3263.2002 |
2. de la Vega H, Specht CA, Semino CE, Robbins PW, Eichinger D, Caplivski D, Ghosh S, and Samuelson J. (1997). Cloning and expression of chitinases of Entamoebae. Mol Biochem Parasitol. 1997;85(2):139-47. DOI:10.1016/s0166-6851(96)02817-4 |
3. Chatterjee A, Ghosh SK, Jang K, Bullitt E, Moore L, Robbins PW, and Samuelson J. (2009). Evidence for a "wattle and daub" model of the cyst wall of entamoeba. PLoS Pathog. 2009;5(7):e1000498. DOI:10.1371/journal.ppat.1000498 |
4. Ralston KS, Solga MD, Mackey-Lawrence NM, Somlata, Bhattacharya A, and Petri WA Jr. (2014). Trogocytosis by Entamoeba histolytica contributes to cell killing and tissue invasion. Nature. 2014;508(7497):526-30. DOI:10.1038/nature13242 |
5. Van Dellen KL, Bulik DA, Specht CA, Robbins PW, and Samuelson JC. (2006). Heterologous expression of an Entamoeba histolytica chitin synthase in Saccharomyces cerevisiae. Eukaryot Cell. 2006;5(1):203-6. DOI:10.1128/EC.5.1.203-206.2006 |
6. Das S, Van Dellen K, Bulik D, Magnelli P, Cui J, Head J, Robbins PW, and Samuelson J. (2006). The cyst wall of Entamoeba invadens contains chitosan (deacetylated chitin). Mol Biochem Parasitol. 2006;148(1):86-92. DOI:10.1016/j.molbiopara.2006.03.002 |
7. Frisardi M, Ghosh SK, Field J, Van Dellen K, Rogers R, Robbins P, and Samuelson J. (2000). The most abundant glycoprotein of amebic cyst walls (Jacob) is a lectin with five Cys-rich, chitin-binding domains. Infect Immun. 2000;68(7):4217-24. DOI:10.1128/IAI.68.7.4217-4224.2000 |
8. Van Dellen KL, Chatterjee A, Ratner DM, Magnelli PE, Cipollo JF, Steffen M, Robbins PW, and Samuelson J. (2006). Unique posttranslational modifications of chitin-binding lectins of Entamoeba invadens cyst walls. Eukaryot Cell. 2006;5(5):836-48. DOI:10.1128/EC.5.5.836-848.2006 |
9. Ghosh SK, Van Dellen KL, Chatterjee A, Dey T, Haque R, Robbins PW, and Samuelson J. (2010). The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic. PLoS Negl Trop Dis. 2010;4(7):e750. DOI:10.1371/journal.pntd.0000750 |
10. Ali IK, Haque R, Siddique A, Kabir M, Sherman NE, Gray SA, Cangelosi GA, and Petri WA Jr. (2012). Proteomic analysis of the cyst stage of Entamoeba histolytica. PLoS Negl Trop Dis. 2012;6(5):e1643. DOI:10.1371/journal.pntd.0001643 |
11. Samuelson J and Robbins P. (2011). A simple fibril and lectin model for cyst walls of Entamoeba and perhaps Giardia. Trends Parasitol. 2011;27(1):17-22. DOI:10.1016/j.pt.2010.09.002 |
12. Ehrenkaufer GM, Weedall GD, Williams D, Lorenzi HA, Caler E, Hall N, and Singh U. (2013). The genome and transcriptome of the enteric parasite Entamoeba invadens, a model for encystation. Genome Biol. 2013;14(7):R77. DOI:10.1186/gb-2013-14-7-r77 |