User:Birgitte Zeuner

Assistant Professor at the Department of Biotechnology and Biomedicine, Technical University of Denmark. My ORCID is here.

Background

I obtained an MSc degree in Biotechnology at Department of Chemical and Biochemical Engineering, Technical University of Denmark in 2009. I continued my PhD studies there under the supervision of Anne S. Meyer, where I worked with CE1 feruloyl esterases to catalyze transesterification in ionic liquids [1, 2], as well as with GH33 sialidases for transsialylation [3, 4]. Starting from solvent engineering and reaction design to improve enzyme-catalysed synthesis, my work has since evolved to include protein engineering and enzyme discovery. My post doc studies included a lot of carbohydrate analysis (HPAEC-PAD) and work on GH33 sialidases [5, 6, 7], GH29 α-L-fucosidases [8, 9, 10], GH20 β-N-acetylhexosaminidases [11, 12], and other GH families [13] for transglycosylation to yield human milk oligosaccharide structures. In 2018, I was appointed Assistant Professor in Enzyme Technology for Oligosaccharide Synthesis at Department of Biotechnology and Biomedicine, Technical University of Denmark. My current work focuses on transglycosylation [10, 14, 15, 16]. As GH-catalysed synthesis is not always a walk in the park, I sometimes spend time off from that to deal with targeted carbohydrate breakdown using PL1 pectin lyases [17].

Selected papers

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1. Zeuner, B., Ståhlberg, T., van Buu, O.N., Kunov-Kruse, A.J., Riisager, A., Meyer, A.S. (2011) Dependency of the hydrogen bonding capacity of the solvent anion on the thermal stability of feruloyl esterases in ionic liquid systems. Green Chem. 13, 1550-1557.DOI: 10.1039/C1GC15115K

7. Zeuner, B., González-Delgado, I., Holck, J., Morales, G., López-Muñoz, M.J., Segura, Y., Meyer, A.S., Mikkelsen, J.D. (2017) Characterization and immobilization of engineered sialidases from Trypanosoma rangeli for transsialylation. AIMS Mol. Sci. 4(2), 140-163.DOI: 10.3934/molsci.2017.2.140

10. Zeuner, B., Meyer, A.S. (2020) Enzymatic transfucosylation for synthesis of human milk oligosaccharides. Carbohydr. Res.DOI: 10.1016/j.carres.2020.108029

16. Muschiol, J., Vuillemin, M., Meyer, A.S., Zeuner, B. (2020) β-N-Acetylhexosaminidases for carbohydrate synthesis via trans-glycosylation. Catalysts 10, 365.DOI: 10.3390/catal10040365