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Difference between revisions of "Polysaccharide Lyase Family 30"
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== Substrate specificities == | == Substrate specificities == | ||
| − | + | PL30 is a small family (38 sequences), exclusively bacterial belonging to the phylum ''Bacteroidetes'' ([http://www.cazy.org/PL30.html PL30]) <cite>Drula2022</cite>. The founding member of family 30, and the only one characterized, belongs to ''Bacteroides cellulosilyticus WH2 (BcellWH2_02524, ''WP_029426181.1). The activity was measured against hyaluronic acid by following the absorbance at 232nm. The increase in absorbance confirms that ''BcellWH2_02524 ''is a hyaluronan lyase ([https://www.enzyme-database.org/query.php?ec=4.2.2.1 4.2.2.1]), probably using the by b-elimination process like all PL families <cite>Helbert2019</cite>. | |
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== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
| − | + | Not Determined | |
== Catalytic Residues == | == Catalytic Residues == | ||
| − | + | Not Determined | |
== Three-dimensional structures == | == Three-dimensional structures == | ||
| − | + | Unsolved | |
== Family Firsts == | == Family Firsts == | ||
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== References == | == References == | ||
<biblio> | <biblio> | ||
| − | # | + | |
| − | # | + | #Drula2022 pmid=34850161 |
| + | |||
| + | #Helbert2019 pmid=30850540 | ||
</biblio> | </biblio> | ||
[[Category:Polysaccharide Lyase Families|PL030]] | [[Category:Polysaccharide Lyase Families|PL030]] | ||
Revision as of 05:16, 23 August 2024
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
| Polysaccharide Lyase Family PL30 | |
| 3D Structure | |
| Mechanism | |
| Charge neutraliser | |
| Active site residues | |
| CAZy DB link | |
| https://www.cazy.org/PL30.html | |
Substrate specificities
PL30 is a small family (38 sequences), exclusively bacterial belonging to the phylum Bacteroidetes (PL30) [1]. The founding member of family 30, and the only one characterized, belongs to Bacteroides cellulosilyticus WH2 (BcellWH2_02524, WP_029426181.1). The activity was measured against hyaluronic acid by following the absorbance at 232nm. The increase in absorbance confirms that BcellWH2_02524 is a hyaluronan lyase (4.2.2.1), probably using the by b-elimination process like all PL families [2].
Kinetics and Mechanism
Not Determined
Catalytic Residues
Not Determined
Three-dimensional structures
Unsolved
Family Firsts
- First stereochemistry determination
- Content is to be added here.
- First catalytic nucleophile identification
- Content is to be added here.
- First general acid/base residue identification
- Content is to be added here.
- First 3-D structure
- Content is to be added here.
References
- Drula E, Garron ML, Dogan S, Lombard V, Henrissat B, and Terrapon N. (2022). The carbohydrate-active enzyme database: functions and literature. Nucleic Acids Res. 2022;50(D1):D571-D577. DOI:10.1093/nar/gkab1045 |
- Helbert W, Poulet L, Drouillard S, Mathieu S, Loiodice M, Couturier M, Lombard V, Terrapon N, Turchetto J, Vincentelli R, and Henrissat B. (2019). Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space. Proc Natl Acad Sci U S A. 2019;116(13):6063-6068. DOI:10.1073/pnas.1815791116 |