User:Yaoguang Chang

Yaoguang Chang obtained his Ph.D. degree from the Ocean University of China and was a visiting scholar at the University of Massachusetts Amherst. At present, he is a professor at the College of Food Science and Engineering, Ocean University of China. His research interests involve the gene-mining and characterization of CAZymes and carbohydrate-binding modules for marine polysaccharides. By utilizing the enzymes and binding proteins as critical tools, novel identification, quantification, and modification methods of marine polysaccharides were established, which would consequently facilitate the development and application of promising functional polysaccharides from the ocean. His research group discovered the first member of GH168, GH174, and CBM92 families, and contributed to the studies related to members of the following families:

GH16: κ-carrageenase Cgk16A [1], βκ-carrageenase Cgbk16A_Wf [2], β-porphyranase Por16A_Wf [3], and β-porphyranase Por16C_Wf [4] GH29: fucosidase Alf1_Wf [5] GH82: ι-carrageenase Cgi82A [6] GH86: β-agarase Aga86A_Wa [7-8] GH168: endo-1,3-fucanase Fun168A [9] GH174: endo-1,3-fucanase Fun174A [10] PL7: alginate lyase Aly7B_Wf [11] CBM16: alginate-binding CBM [12] CBM47: sulfated fucan-binding CBM WfCBM47 [13] CBM70: hyaluronic acid-binding CBM SrCBM70 [14] CBM92: carrageenan-binding CBM Cgk16A-CBM92 [15] CBMnc: sulfated fucan-binding CBM Fun174A-CBM [16]

Some of the above biotechnological tools have been successfully integrated with glycomics [17-18], lateral flow immunoassay [14], and other techniques, and served in the structural and chemical investigation of marine polysaccharides. And some enzymes have been employed to produce low molecular weight polysaccharides and oligosaccharides with verified bioactivities [19-20].

References

1. pmid= 29355636

2. pmid= 34421852

3. pmid= 31352784

4. pmid= 32520542

5. pmid= 27576198

6. pmid= 28760444

7. pmid= 32578425

8. pmid= 36746585

9. pmid= 32849348

10. pmid= 36746582

11. pmid= 30248453

12. Mei X, Chang Y, Shen J, Zhang Y, and Xue C. (2020). Expression and characterization of a novel alginate-binding protein: A promising tool for investigating alginate. Carbohydr Polym. 2020;246:116645. DOI:10.1016/j.carbpol.2020.116645 |
13. Mei X, Chang Y, Shen J, Zhang Y, Chen G, Liu Y, and Xue C. (2022). Characterization of a sulfated fucan-specific carbohydrate-binding module: A promising tool for investigating sulfated fucans. Carbohydr Polym. 2022;277:118748. DOI:10.1016/j.carbpol.2021.118748 |
13. Mei X, Sun M, Zhang Y, Shen J, Li J, Xue C, and Chang Y. (2022). Establishment of a carbohydrate binding module-based lateral flow immunoassay method for identifying hyaluronic acid. Int J Biol Macromol. 2022;223(Pt A):1180-1185. DOI:10.1016/j.ijbiomac.2022.11.122 |
13. Mei X, Chang Y, Shen J, Zhang Y, Han J, and Xue C. (2022). Characterization of a Novel Carrageenan-Specific Carbohydrate-Binding Module: a Promising Tool for the In Situ Investigation of Carrageenan. J Agric Food Chem. 2022;70(29):9066-9072. DOI:10.1021/acs.jafc.2c03139 |
14. Mei X, Liu G, Shen J, Chen G, Zhang Y, Xue C, and Chang Y. (2023). Discovery of a sulfated fucan-specific carbohydrate-binding module: The first member of a new carbohydrate-binding module family. Int J Biol Macromol. 2023;238:124037. DOI:10.1016/j.ijbiomac.2023.124037 |

15. pmid= 34420739

16. pmid= 37059545

17. pmid= 35667104

18. pmid= 34415766