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Difference between revisions of "Glycoside Hydrolase Family 19"
Line 47: | Line 47: | ||
== Family Firsts == | == Family Firsts == | ||
− | First primary sequence determination | + | First primary sequence determination: |
Bean leaf chitinase (Broglie et al., 1986) | Bean leaf chitinase (Broglie et al., 1986) | ||
− | First stereochemistry determination | + | First stereochemistry determination: |
Yam chitinase, by NMR (Fukamizo et al., 1995) and Bean chitinase, by HPLC (Iseli et al., 1996) | Yam chitinase, by NMR (Fukamizo et al., 1995) and Bean chitinase, by HPLC (Iseli et al., 1996) | ||
− | First general base residue identification | + | First general base residue identification: |
Chitinase from barley; determination by site-directed mutagenesis (Andersen et al., 1997), structural analysis (Hart et al., 1993) and modelling (Brameld and Goddard, 1998). Additional support from structure determination and modelling of a papaya chitinase (Huet et al., 2008). | Chitinase from barley; determination by site-directed mutagenesis (Andersen et al., 1997), structural analysis (Hart et al., 1993) and modelling (Brameld and Goddard, 1998). Additional support from structure determination and modelling of a papaya chitinase (Huet et al., 2008). | ||
− | First general acid residue identification | + | First general acid residue identification: |
Chitinase from barley; determination by site-directed mutagenesis (Andersen et al., 1997), structural analysis (Hart et al., 1993) and modelling (Brameld and Goddard, 1998). Additional support from structure determination and modelling of a papaya chitinase (Huet et al., 2008). | Chitinase from barley; determination by site-directed mutagenesis (Andersen et al., 1997), structural analysis (Hart et al., 1993) and modelling (Brameld and Goddard, 1998). Additional support from structure determination and modelling of a papaya chitinase (Huet et al., 2008). | ||
− | First 3-D structure | + | First 3-D structure: |
Barley chitinase (Hart et al., 1993). | Barley chitinase (Hart et al., 1993). | ||
Revision as of 13:54, 25 March 2010
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author: ^^^Vincent Eijsink^^^
- Responsible Curator: ^^^Vincent Eijsink^^^
Glycoside Hydrolase Family GHnn | |
Clan | GH-x |
Mechanism | retaining/inverting |
Active site residues | known/not known |
CAZy DB link | |
http://www.cazy.org/fam/GHnn.html |
Substrate specificities
Content is to be added here.
This is an example of how to make references to a journal article [1]. (See the References section below). Multiple references can go in the same place like this [1, 2]. You can even cite books using just the ISBN [3]. References that are not in PubMed can be typed in by hand [4].
Kinetics and Mechanism
Content is to be added here.
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Content is to be added here.
Family Firsts
First primary sequence determination: Bean leaf chitinase (Broglie et al., 1986)
First stereochemistry determination: Yam chitinase, by NMR (Fukamizo et al., 1995) and Bean chitinase, by HPLC (Iseli et al., 1996)
First general base residue identification: Chitinase from barley; determination by site-directed mutagenesis (Andersen et al., 1997), structural analysis (Hart et al., 1993) and modelling (Brameld and Goddard, 1998). Additional support from structure determination and modelling of a papaya chitinase (Huet et al., 2008).
First general acid residue identification: Chitinase from barley; determination by site-directed mutagenesis (Andersen et al., 1997), structural analysis (Hart et al., 1993) and modelling (Brameld and Goddard, 1998). Additional support from structure determination and modelling of a papaya chitinase (Huet et al., 2008).
First 3-D structure: Barley chitinase (Hart et al., 1993).