CAZypedia needs your help!
We have many unassigned pages in need of Authors and Responsible Curators. See a page that's out-of-date and just needs a touch-up? - You are also welcome to become a CAZypedian. Here's how.
Scientists at all career stages, including students, are welcome to contribute.
Learn more about CAZypedia's misson here and in this article.
Totally new to the CAZy classification? Read this first.
Difference between revisions of "Glycoside Hydrolases"
(New page: <!-- Sourced from the Template:Biolerplate page by the preloader.php script --> '''The text below is a template to help you create a consistent layout for GH entries. To get an idea of w...) |
|||
| Line 1: | Line 1: | ||
<!-- Sourced from the Template:Biolerplate page by the preloader.php script --> | <!-- Sourced from the Template:Biolerplate page by the preloader.php script --> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
== Overview == | == Overview == | ||
| − | + | Glycoside hydrolases are enzymes that catalyze the hydrolysis of the glycosidic linkage of glycosides, leading to the formation of a simple sugar and the corresponding alglycon. Glycoside hydrolases are also referred to as glycosidases. Glycoside hydrolases are known that can catalyze the hydrolysis of O-, N- and S-linked glycosides. | |
== Classification == | == Classification == | ||
| + | Glycoside hydrolases can be classified in many different ways. The following paragraphs list several different ways, the utility of which depends on the context in which the classification is made and used. | ||
| + | ''exo''- and ''endo''- refers to the ability of a glycoside hydrolase to cleave a substrate at the end (most frequently, but not always the non-reducing end) or within the middle of a chain. For example, most cellulases are ''endo''-acting, whereas LacZ β-galactosidase from ''E. coli'' is ''exo''-acting. | ||
== Mechanism == | == Mechanism == | ||
Revision as of 01:50, 21 August 2008
Overview
Glycoside hydrolases are enzymes that catalyze the hydrolysis of the glycosidic linkage of glycosides, leading to the formation of a simple sugar and the corresponding alglycon. Glycoside hydrolases are also referred to as glycosidases. Glycoside hydrolases are known that can catalyze the hydrolysis of O-, N- and S-linked glycosides.
Classification
Glycoside hydrolases can be classified in many different ways. The following paragraphs list several different ways, the utility of which depends on the context in which the classification is made and used.
exo- and endo- refers to the ability of a glycoside hydrolase to cleave a substrate at the end (most frequently, but not always the non-reducing end) or within the middle of a chain. For example, most cellulases are endo-acting, whereas LacZ β-galactosidase from E. coli is exo-acting.
Mechanism
Sequence-based classification
Glycoside Hydrolase Firsts
- First sterochemistry determination
- Cite some reference here, with a short explanation [1].
- First catalytic nucleophile identification
- First general acid/base residue identification
- First 3-D structure
References
- Comfort DA, Bobrov KS, Ivanen DR, Shabalin KA, Harris JM, Kulminskaya AA, Brumer H, and Kelly RM. (2007). Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Biochemistry. 2007;46(11):3319-30. DOI:10.1021/bi061521n |
[[Category:Glycoside Hydrolase Families]]