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Difference between revisions of "Glycoside Hydrolase Family 117"
| Line 29: | Line 29: | ||
== Substrate specificities == | == Substrate specificities == | ||
| − | The only activity so far identified in this recently discovered family of glycoside hydrolases is that α-1,3-L-(3,6-anhydro)-galactosidase <cite> | + | The only activity so far identified in this recently discovered family of glycoside hydrolases is that α-1,3-L-(3,6-anhydro)-galactosidase <cite>Sugano1994 Suzuki2002 Rebuffet2011</cite>. Nevertheless phylogenetic analysis of this family and activity test on a member from an other clade show that the family GH117 is polyspecific <cite>Rebuffet2011</cite>. |
| Line 37: | Line 37: | ||
== Catalytic Residues == | == Catalytic Residues == | ||
| − | From structural analysis and sequences alignment the catalytic residues have been predicted to be two out of the three acidic residues Asp-97, Asp-252 and Glu-310 (Zg4663 numbering) <cite> | + | From structural analysis and sequences alignment the catalytic residues have been predicted to be two out of the three acidic residues Asp-97, Asp-252 and Glu-310 (Zg4663 numbering) <cite>Rebuffet2011</cite>. |
== Three-dimensional structures == | == Three-dimensional structures == | ||
| − | Zg4663, α-1,3-L-(3,6-anhydro)-galactosidase (AhgA), PDB: [http://www.pdb.org/pdb/explore/explore.do?structureId=3p2n 3P2N] <cite> | + | Zg4663, α-1,3-L-(3,6-anhydro)-galactosidase (AhgA), PDB: [http://www.pdb.org/pdb/explore/explore.do?structureId=3p2n 3P2N] <cite>Rebuffet2011</cite>. |
| Line 48: | Line 48: | ||
;First catalytic nucleophile identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>Sinnott1990</cite>. | ;First catalytic nucleophile identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>Sinnott1990</cite>. | ||
;First general acid/base residue identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>He1999</cite>. | ;First general acid/base residue identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>He1999</cite>. | ||
| − | ;First 3-D structure: Zg4663, α-1,3-L-(3,6-anhydro)-galactosidase (AhgA), PDB: [http://www.pdb.org/pdb/explore/explore.do?structureId=3p2n 3P2N] <cite> | + | ;First 3-D structure: Zg4663, α-1,3-L-(3,6-anhydro)-galactosidase (AhgA), PDB: [http://www.pdb.org/pdb/explore/explore.do?structureId=3p2n 3P2N] <cite>Rebuffet2011</cite>. |
== References == | == References == | ||
<biblio> | <biblio> | ||
| − | # | + | #Sugano1994 pmid=7961439 |
| − | # | + | #Suzuki2002 pmid=16233232 |
| − | # | + | #Rebuffet2011 pmid=21332624 |
#He1999 pmid=9312086 | #He1999 pmid=9312086 | ||
#StickWilliams isbn=978-0-240-52118-3 | #StickWilliams isbn=978-0-240-52118-3 | ||
Revision as of 00:16, 5 May 2011
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author: ^^^Etienne Rebuffet^^^
- Responsible Curator: ^^^Mirjam Czjzek^^^
| Glycoside Hydrolase Family GH117 | |
| Clan | None |
| Mechanism | Not known |
| Active site residues | Not known |
| CAZy DB link | |
| https://www.cazy.org/GH117.html | |
Substrate specificities
The only activity so far identified in this recently discovered family of glycoside hydrolases is that α-1,3-L-(3,6-anhydro)-galactosidase [1, 2, 3]. Nevertheless phylogenetic analysis of this family and activity test on a member from an other clade show that the family GH117 is polyspecific [3].
Kinetics and Mechanism
Mechanism of glycoside hydrolase family 117 is still unknown.
Catalytic Residues
From structural analysis and sequences alignment the catalytic residues have been predicted to be two out of the three acidic residues Asp-97, Asp-252 and Glu-310 (Zg4663 numbering) [3].
Three-dimensional structures
Zg4663, α-1,3-L-(3,6-anhydro)-galactosidase (AhgA), PDB: 3P2N [3].
Family Firsts
- First stereochemistry determination
- Cite some reference here, with a short (1-2 sentence) explanation [4].
- First catalytic nucleophile identification
- Cite some reference here, with a short (1-2 sentence) explanation [5].
- First general acid/base residue identification
- Cite some reference here, with a short (1-2 sentence) explanation [6].
- First 3-D structure
- Zg4663, α-1,3-L-(3,6-anhydro)-galactosidase (AhgA), PDB: 3P2N [3].
References
- Sugano Y, Kodama H, Terada I, Yamazaki Y, and Noma M. (1994). Purification and characterization of a novel enzyme, alpha-neoagarooligosaccharide hydrolase (alpha-NAOS hydrolase), from a marine bacterium, Vibrio sp. strain JT0107. J Bacteriol. 1994;176(22):6812-8. DOI:10.1128/jb.176.22.6812-6818.1994 |
- Suzuki H, Sawai Y, Suzuki T, and Kawai K. (2002). Purification and characterization of an extracellular alpha-neoagarooligosaccharide hydrolase from Bacillus sp. MK03. J Biosci Bioeng. 2002;93(5):456-63. DOI:10.1016/s1389-1723(02)80092-5 |
- Rebuffet E, Groisillier A, Thompson A, Jeudy A, Barbeyron T, Czjzek M, and Michel G. (2011). Discovery and structural characterization of a novel glycosidase family of marine origin. Environ Microbiol. 2011;13(5):1253-70. DOI:10.1111/j.1462-2920.2011.02426.x |
-
Sinnott, M.L. (1990) Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90, 1171-1202. DOI: 10.1021/cr00105a006
- He S and Withers SG. (1997). Assignment of sweet almond beta-glucosidase as a family 1 glycosidase and identification of its active site nucleophile. J Biol Chem. 1997;272(40):24864-7. DOI:10.1074/jbc.272.40.24864 |
This is an example of how to make references to a journal article [4]. (See the References section below). Multiple references can go in the same place like this [4, 6]. You can even cite books using just the ISBN [7]. References that are not in PubMed can be typed in by hand [5].