Glycoside Hydrolase Family 43

• Author: Satoshi Kaneko
• Responsible Curator: Harry Brumer

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Glycoside Hydrolase Family GH43
Clan	GH-F
Mechanism	inverting
Active site residues	not known
CAZy DB link
http://www.cazy.org/fam/GH43.html

Substrate specificities

GH43 enzymes display a variety of exo-activities in which L-alpha-arabinofuanose, D-beta-galactopyranose and D-beta-xylopyranose residues are hydrolyzed from the non-reducing end of polysaccharides or aryl groups. In addition examples of endo-alpha1,5-arabinanases are also evident in this family

Kinetics and Mechanism

NMR, deploying arabinan as the substrate, showed that an endo-alpha1,5-arabinanase displays a single displacement or inverting mechanism

Family Firsts

First sterochemistry determination

First catalytic nucleophile identification

First general acid/base residue identification

First 3-D structure

alpha-L-Arabinanase from Cellvibrio japonicus [1].

References

1. Nurizzo D, Turkenburg JP, Charnock SJ, Roberts SM, Dodson EJ, McKie VA, Taylor EJ, Gilbert HJ, and Davies GJ. (2002). Cellvibrio japonicus alpha-L-arabinanase 43A has a novel five-blade beta-propeller fold. Nat Struct Biol. 2002;9(9):665-8. DOI:10.1038/nsb835 |