Glycoside Hydrolase Family 121

This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.

• Author: ^^^Kiyotaka Fujita^^^
• Responsible Curator: ^^^Shinya Fushinobu^^^

---

Glycoside Hydrolase Family GH121
Clan	GH-x
Mechanism	retaining
Active site residues	known/not known
CAZy DB link
https://www.cazy.org/GH121.html

Substrate specificities

This family of glycoside hydrolases contains β-L-arabinobiosidase, which was recently established for HypBA2 from Bifidobacterium longum JCM 1217[1].

Kinetics and Mechanism

HypBA2 is a retaining enzyme.

Catalytic Residues

Content is to be added here.

Three-dimensional structures

Content is to be added here.

Family Firsts

First stereochemistry determination

This was determined with HypBA2 enzyme by measurement of glycosyl transfer reactions to methanol and the ¹H-NMR and ¹³C-NMR spectra.

First catalytic nucleophile identification

Content is to be added here.

First general acid/base residue identification

Content is to be added here.

First 3-D structure

Content is to be added here.

References

1. Fujita K, Sakamoto S, Ono Y, Wakao M, Suda Y, Kitahara K, and Suganuma T. (2011). Molecular cloning and characterization of a beta-L-Arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family. J Biol Chem. 2011;286(7):5143-50. DOI:10.1074/jbc.M110.190512 |