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Difference between revisions of "Polysaccharide Lyase Family 7"

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== Substrate specificities ==
 
== Substrate specificities ==
The polysaccharide lyase family 7 (PL7) contains 5 subfamilies <cite>Lombard2010</cite>. All characterized members were active on alginate the anionic, gel forming polysaccharide from brown seaweed such as kelps. Alginate consists of beta-D-mannuronate and alpha-L-guluronate, which occur in homogenous or heterogenous blocks.  Bacteria also synthesize alginate for which alginate lyases not only occur in marine but also in terrestrial microbes. Some PL7 prefer regions made of mannuronate others prefer guluronate and the rest does not distinguish.  
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The polysaccharide lyase family 7 (PL7) contains 5 subfamilies <cite>Lombard2010</cite>. All characterized members were active on alginate the anionic, gel forming polysaccharide from brown seaweed such as kelps. Alginate consists of beta-D-mannuronate and alpha-L-guluronate, which occur in homogenous or heterogenous blocks. Hence, PL7 can be mannuronate, guluronate or mixed link lyases.  Bacteria also synthesize alginate for which alginate lyases not only occur in marine but also in terrestrial microbes.
  
  

Revision as of 09:39, 16 February 2015

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Polysaccharide Lyase Family PL7
3D Structure β jelly roll
Mechanism β-elimination
Active site residues known
CAZy DB link
https://www.cazy.org/PL7.html

Substrate specificities

The polysaccharide lyase family 7 (PL7) contains 5 subfamilies [1]. All characterized members were active on alginate the anionic, gel forming polysaccharide from brown seaweed such as kelps. Alginate consists of beta-D-mannuronate and alpha-L-guluronate, which occur in homogenous or heterogenous blocks. Hence, PL7 can be mannuronate, guluronate or mixed link lyases. Bacteria also synthesize alginate for which alginate lyases not only occur in marine but also in terrestrial microbes.


Catalytic Residues

Three-dimensional structures

Family Firsts

References

  1. Lombard V, Bernard T, Rancurel C, Brumer H, Coutinho PM, and Henrissat B. (2010). A hierarchical classification of polysaccharide lyases for glycogenomics. Biochem J. 2010;432(3):437-44. DOI:10.1042/BJ20101185 | PubMed ID:20925655 [Lombard2010]