Carbohydrate Binding Module Family 3

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• Author:
• Responsible Curator: ^^^Ed Bayer^^^

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CAZy DB link

https://www.cazy.org/CBM11.html

Ligand specificities

CBM3 is a Gram-positive bacterial family that comprises around 150 amino acids. The family is divided into four subgroups, CBM3a-d. The major ligand recognised by CBM3as and CBM3bs is crystalline cellulose with an affinity (K_D) of 0.4 µM determined by depletion isotherms [1]. Isothermal titration calorimetry showed that binding to crystalline cellulose was entropically driven consistent with apolar interactions resulting in the release of caged water molecules from a ligand with a restricted conformation [2, 3]. CBM3s that bind to crystalline cellulose also interact with chitin and xyloglucan with an affinity ~500 lower than for crystalline cellulose. Interaction with the soluble polysaccharide was enthalpically driven with changes in entropy having a negative impact on affinity[2, 3]. The site of binding of a CBM3 from the Clostridium thermocellulum scaffoldin (CipA) to crystalline cellulose was determined by transmission electron microscopy with detection of the protein by immuno-gold labelling. The data showed that the CBM3 bound to the 110 face of Valonia cellulose [4]. The binding profile and site of cellulose recognition show that CBM3s are type A modules. The three CBM3s from anti-sigma sensors displayed different specificities; Cthe_0059 CBM3b bound to a range of plant cell wall polysaccharides (PCWPs), Cthe_0404 CBM3b interacted weakly to xyloglucan but not to any other PCWP, and Cthe_0267 CBM3 bound primarily to crystalline and amorphous cellulose [5, 6].

Structural Features

The crystal structure of CBM3 from the C. thermocellum scaffoldin CipA revealed a classical β-jelly-roll fold consisting of nine β-strands in two antiparallel β-sheets comprising four (1, 2, 7, 4; β-sheet 1) and five (9, 8, 3, 6, 5; β-sheet 2) β strands, respectively (Figure 1 [7]. b-sheet 1 forms a flat surface that contains a linear array of five residues that presents a planar hydrophobic surface comprising a His, Trp, Tyr and an Arg-Asp ion pair. The residues in the planar strip were predicted to make apolar interactions with glucose molecules n, n+1, n+3 and n+5, consistent with mutagenesis data showing that each of the five amino acids played an important role in binding cellulose [8]. Structures of CBMbs from other cellulosome-producing species followed that reinforced the original structural findings [9, 10, 11]. In other CBM3s that bind to cellulose, such as in the anti-σ-cell surface sensor RsgI1 (Cthe_0059), the His and ion pair are replaced by a Tyr and Phe, thus the hydrophobic planar binding site comprises four aromatic amino acids [6]. In a second cellulose-binding CBM3 located in an Rsgl sensor (Rsgl2, Cthe_0267), the aromatic planar strip is truncated, but lies planar with a hydrophobic protruding loop that is predicted to contribute to the cellulose binding site of the protein, similar to a group d CBM3 present in a GH48 exo-cellulase [12]. In addition to the hydrophobic strips it has also been proposed that highly conserved polar residues may be able to make productive hydrogen bonds with two additional cellulose chains in the microfibril [6, 7].

Functionalities

Content in this section should include, in paragraph form, a description of:

• Functional role of CBM: Describe common functional roles such as targeting, disruptive, anchoring, proximity/position on substrate.
• Most Common Associated Modules: 1. Glycoside Hydrolase Activity; 2. Additional Associated Modules (other CBM, FNIII, cohesin, dockerins, expansins, etc.)
• Novel Applications: Include here if CBM has been used to modify another enzyme, or if a CBM was used to label plant/mammalian tissues? Etc.

Family Firsts

First Identified

Insert archetype here, possibly including very brief synopsis.

First Structural Characterization

Insert archetype here, possibly including very brief synopsis.

References

13. Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, and Henrissat B. (2009). The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics. Nucleic Acids Res. 2009;37(Database issue):D233-8. DOI:10.1093/nar/gkn663 |

14. Davies, G.J. and Sinnott, M.L. (2008) Sorting the diverse: the sequence-based classifications of carbohydrate-active enzymes. The Biochemist, vol. 30, no. 4., pp. 26-32. Download PDF version.

15. Boraston AB, Bolam DN, Gilbert HJ, and Davies GJ. (2004). Carbohydrate-binding modules: fine-tuning polysaccharide recognition. Biochem J. 2004;382(Pt 3):769-81. DOI:10.1042/BJ20040892 |
16. Hashimoto H (2006). Recent structural studies of carbohydrate-binding modules. Cell Mol Life Sci. 2006;63(24):2954-67. DOI:10.1007/s00018-006-6195-3 |
17. Shoseyov O, Shani Z, and Levy I. (2006). Carbohydrate binding modules: biochemical properties and novel applications. Microbiol Mol Biol Rev. 2006;70(2):283-95. DOI:10.1128/MMBR.00028-05 |
18. Guillén D, Sánchez S, and Rodríguez-Sanoja R. (2010). Carbohydrate-binding domains: multiplicity of biological roles. Appl Microbiol Biotechnol. 2010;85(5):1241-9. DOI:10.1007/s00253-009-2331-y |