Glycosyltransferase Family 42

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• Author: ^^^Warren Wakarchuk^^^
• Responsible Curator: ^^^Warren Wakarchuk^^^

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Glycosyltransferase Family GT42
Clan	GT-x
Mechanism	Inverting. CMP-NeuAc α-2,3-sialyltransferase (EC 2.4.99.-); donor is CMP-β-Neu5Ac
Active site residues	known
CAZy DB link
http://www.cazy.org/fam/GT42.html

Substrate specificities

The glycosyltransferases in family GT42 were originally examined from isolates of C. jejuni that express a number of ganglioside mimics, some of which have an α2,8-α2,3 linked di-sialic acid moiety [1]. The CjGT42 known as Cst-II was found as part of the lipooligosaccharide (LOS) biosynthesis operon [2], which facilitated the correlation of gene content to LOS structure. Based on the LOS structures which contained both α2,3 and α2,8 linked sialic acid it was predicted that there should be two sialyltransferases, but the surprising finding was that a single CjGT-42 enzyme, Cst-II, was making both linkages [3]. This bi-functional enzyme has been shown to be a determinant in the development of post-infectious neuropathies (Guillain-Barré syndrome), due to an anti-ganglioside antibody response in some patients who were infected with C. jejuni [4, 5]. Some species of Campylobacter express a second family GT42 enzyme known as Cst-I, which was in fact cloned first, but it has been shown not to be involved in LOS biosynthesis on the basis of gene knockouts in strains with both cst genes (Michel Gilbert, personal communication). Cst-I contains an extended C-terminal domain of unknown function, and the target acceptor of this second CjGT-42 is not known at present. The GT42 family also contains members from H. influenzae and P. multocida, in which the GT42 enzymes are one of two or three resident sialyltransferases. In H. influenzae the first GT-42 enzyme to be described was the α2,3-sialyltransferase Lic3A [6], followed by a second a2,3/2,8-bi-functional version known as Lic3B [7]. In H. influenzae the in vivo acceptor for Lic3A/B is the simple disaccharide lactose in contrast to the more complex ganglioside mimics seen in C. jejuni [8]. At present there are uncharacterized members of GT42 from Helicobacter acinonychis str. Sheeba, H. mustelae that were identified through genome sequencing, but which have not been linked to specific sialylated structures.

Kinetics and Mechanism

Detailed kinetic studies have revealed that this enzyme follows an unusual steady state iso ordered bi bi kinetic mechanism for carrying out sialyl transfer with inversion of anomeric stereochemistry [9]. His188 appears to be the catalytic general base, which is somewhat unusual since this role is generally performed by Glu or Asp residues. Direct measurement of its pK_a value by NMR showed the pK_a agrees with that deduced from the pH dependence of this reaction. This required the generation of an active monomeric form of the tetrameric enzyme [9]. Chemical rescue of the H188A mutant was performed with nucleophilic anions adding weight to the assignment of the function, and further supporting the S_N2-like inverting mechanism.

Catalytic Residues

His188 in CjGT42 Cst-II_OH4383 appears to be the catalytic general base [9]. The amino acids Asn51 and Leu54 had the most effect on the bi-functional activity of Cst-II, but only Asn51 was shown to be critical for the α2,8-sialyltransferase activity in this variant of Cst-II. There are members of GT42 which have an Asn at an equivalent sequence position that are not bi-functional, but they have much lower overall sequence homolgy with Cst-II_OH4384.

Three-dimensional structures

The CjGT42 enzymes Cst-I and Cst-II were the first sialyltransferases whose 3D structure was determined by X-ray crystallography [10, 11], and they share the same basic structure. GT42 enzymes have a modified GT-A type fold characterized by a single α/β Rossmann fold nucleotide binding domain, and a flexible lid domain composed of a coil and two helices. Unlike the typical GT-A fold, CjGT-42 enzymes lack the conserved DXD motif and are metal-ion independent. The enzyme structure revealed a tetramer in which each monomer carries an independent active site, and examination of the active site residues suggested a conserved histidine, His188 in Cst-II, as the catalytic base.

Glycosyltransferase structures often have a “lid” domain, which is a mobile loop that undergoes a conformational change upon binding a substrate [12]. The lid domain in Cst-I and Cst-II contains residues which interact with the donor and/or acceptor. A common feature of transferase substrate binding is the role of aromatic residues stacking onto the base of the sugar-nucleotide. In Cst-I the aromatic residue is Tyr171 and in Cst-II it is Tyr156, both of which are conserved in GT-42 and occur in the lid domain. There is no structure of a ternary complex for GT-42 so it is not possible to know all of the interactions that are involved in substrate binding, but there are donor complexes with the incompetent donor CMP-3-fluoro-Neu5Ac and acceptor models have been examined to predict interactions [11]. In Cst-I, Phe190 appears to prevent sialylated acceptors from binding in the active site. In Cst-II, this Phe residue is replaced by Ala175, which opens up a pocket for the carboxyl group of a modelled sialyl-lactose acceptor [11].

• PDB ID 1RO8 from GT42 (click images for large versions)
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Family Firsts

First catalytic residue identification

His188 is the catalytic base in the Campylobacter jejuni GT42 known as Cst-II [9]. His201 is the catalytic base inCampylobacter jejuni Cst-II.

First substrate specficity determination

GT42 known as Cst-IThe CjGT-42 enzyme Cst-II from strain OH4384, was the demonstrated to be first sialyltransferase which could make both α2,3 and α2,8 linkages [3]. This bi-functional activity is unique to GT-42.

First 3-D structure

The CjGT-42 enzymes Cst-I and Cst-II were the first sialyltransferases whose structure was determined by X-ray crystallography [10, 11].

References

1. Irving Nachamkin, Christine M. Szymanski, and Martin J. Blaser. (2008) Campylobacter. Amer Society for Microbiology.

   Book chapter: Gilbert, M., Parker, C.T., and Moran, A. P. Campylobacter jejuni Lipooligosaccharides: Structure and Function. In Campylobacter 3rd Edition, editors Irving Nachamkin, Christine M. Szymanski, Martin J. Blaser, ASM press, Washington D.C. pp 438-504, 2008

2. Gilbert M, Brisson JR, Karwaski MF, Michniewicz J, Cunningham AM, Wu Y, Young NM, and Wakarchuk WW. (2000). Biosynthesis of ganglioside mimics in Campylobacter jejuni OH4384. Identification of the glycosyltransferase genes, enzymatic synthesis of model compounds, and characterization of nanomole amounts by 600-mhz (1)h and (13)c NMR analysis. J Biol Chem. 2000;275(6):3896-906. DOI:10.1074/jbc.275.6.3896 |
3. Gilbert M, Karwaski MF, Bernatchez S, Young NM, Taboada E, Michniewicz J, Cunningham AM, and Wakarchuk WW. (2002). The genetic bases for the variation in the lipo-oligosaccharide of the mucosal pathogen, Campylobacter jejuni. Biosynthesis of sialylated ganglioside mimics in the core oligosaccharide. J Biol Chem. 2002;277(1):327-37. DOI:10.1074/jbc.M108452200 |
4. Koga M, Takahashi M, Masuda M, Hirata K, and Yuki N. (2005). Campylobacter gene polymorphism as a determinant of clinical features of Guillain-Barré syndrome. Neurology. 2005;65(9):1376-81. DOI:10.1212/01.wnl.0000176914.70893.14 |
5. van Belkum A, van den Braak N, Godschalk P, Ang W, Jacobs B, Gilbert M, Wakarchuk W, Verbrugh H, and Endtz H. (2001). A Campylobacter jejuni gene associated with immune-mediated neuropathy. Nat Med. 2001;7(7):752-3. DOI:10.1038/89831 |
6. Hood DW, Cox AD, Gilbert M, Makepeace K, Walsh S, Deadman ME, Cody A, Martin A, Månsson M, Schweda EK, Brisson JR, Richards JC, Moxon ER, and Wakarchuk WW. (2001). Identification of a lipopolysaccharide alpha-2,3-sialyltransferase from Haemophilus influenzae. Mol Microbiol. 2001;39(2):341-50. DOI:10.1046/j.1365-2958.2001.02204.x |
7. Fox KL, Cox AD, Gilbert M, Wakarchuk WW, Li J, Makepeace K, Richards JC, Moxon ER, and Hood DW. (2006). Identification of a bifunctional lipopolysaccharide sialyltransferase in Haemophilus influenzae: incorporation of disialic acid. J Biol Chem. 2006;281(52):40024-32. DOI:10.1074/jbc.M602314200 |
8. Schweda EK, Richards JC, Hood DW, and Moxon ER. (2007). Expression and structural diversity of the lipopolysaccharide of Haemophilus influenzae: implication in virulence. Int J Med Microbiol. 2007;297(5):297-306. DOI:10.1016/j.ijmm.2007.03.007 |
9. Chan PH, Lairson LL, Lee HJ, Wakarchuk WW, Strynadka NC, Withers SG, and McIntosh LP. (2009). NMR spectroscopic characterization of the sialyltransferase CstII from Campylobacter jejuni: histidine 188 is the general base. Biochemistry. 2009;48(47):11220-30. DOI:10.1021/bi901606n |
10. Chiu CP, Watts AG, Lairson LL, Gilbert M, Lim D, Wakarchuk WW, Withers SG, and Strynadka NC. (2004). Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog. Nat Struct Mol Biol. 2004;11(2):163-70. DOI:10.1038/nsmb720 |
11. Chiu CP, Lairson LL, Gilbert M, Wakarchuk WW, Withers SG, and Strynadka NC. (2007). Structural analysis of the alpha-2,3-sialyltransferase Cst-I from Campylobacter jejuni in apo and substrate-analogue bound forms. Biochemistry. 2007;46(24):7196-204. DOI:10.1021/bi602543d |
12. Lairson LL, Henrissat B, Davies GJ, and Withers SG. (2008). Glycosyltransferases: structures, functions, and mechanisms. Annu Rev Biochem. 2008;77:521-55. DOI:10.1146/annurev.biochem.76.061005.092322 |