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Difference between revisions of "Carbohydrate Binding Module Family 92"
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== Family Firsts == | == Family Firsts == | ||
;First Identified | ;First Identified | ||
| − | The first member Cgk16A-CBM92 is a component of a κ-carrageenase Cgk16A [2], which was discovered from a marine bacterium ''Wenyingzhuangia aestuarii'' OF219. | + | ;The first member Cgk16A-CBM92 is a component of a κ-carrageenase Cgk16A [2], which was discovered from a marine bacterium ''Wenyingzhuangia aestuarii'' OF219. |
== References == | == References == | ||
Revision as of 00:51, 27 January 2023
Cite error: Invalid <ref> tag; refs with no name must have content
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| CAZy DB link | |
| https://www.cazy.org/CBM92.html |
Ligand specificities
The first and only hereinto characterized member in the CBM92 family is Cgk16A-CBM92 [1]. The CBM could specifically bind to carrageenan and showed no significant difference in the affinity to κ- and ι-carrageenan.
Structural Features
No three-dimensional structure has been solved in this CBM family at present.
Functionalities
A carrageenan-specific fluorescent probe was successfully constructed by fusing Cgk16A-CBM92 with a green fluorescent protein. The in situ visualization of carrageenan in red alga Kappaphycus alvarezii was realized by utilizing the fluorescent probe [1].
Family Firsts
- First Identified
- The first member Cgk16A-CBM92 is a component of a κ-carrageenase Cgk16A [2], which was discovered from a marine bacterium Wenyingzhuangia aestuarii OF219.
References
[1] X. Mei, Y. Chang, J. Shen, Y. Zhang, J. Han, C. Xue. (2022). Characterization of a novel carrageenan-specific carbohydrate- binding module: a promising tool for the in situ investigation of carrageenan. J. Agric. Food Chem. 2022;70: 9066-9072. DOI: 10.1021/acs.jafc.2c03139 | PUBMED ID: 35830544 [Mei2022] [2] J. Shen, Y. Chang, F. Chen, S. Dong. (2018). Expression and characterization of a kappa-carrageenase from marine bacterium Wenyingzhuangia aestuarii OF219: a biotechnological tool for the depolymerization of kappa-carrageenan. Int. J. Biol. Macromol. 2018;112: 93-100. DOI: 10.1016/j.ijbiomac.2018.01.075 | PUBMED ID: 29355636 [Shen2018]