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Difference between revisions of "Glycoside Hydrolase Family 46"
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== Substrate specificities == | == Substrate specificities == | ||
| − | Glycoside hydrolases of family 46 are essentially ''endo''-beta-1,4-chitosanases (EC 3.2.1.132) that hydrolyze various links in chitosan, a polymer of beta-1,4-linked D-glucosamine (GlcN) units with a variable content (mostly 0 - 35%) of N-acetyl-D-glucosamine (GlcNAc) <cite>Yabuki1988 Boucher1992</cite>. Among the four types of links occurring between these two kinds of subunits, all the enzymes examined for their cleavage specificity recognized productively the GlcN-GlcN links. Furthermore, the chitosanase from ''Bacillus circulans'' MH-K1 | + | Glycoside hydrolases of family 46 are essentially ''endo''-beta-1,4-chitosanases (EC 3.2.1.132) that hydrolyze various links in chitosan, a polymer of beta-1,4-linked D-glucosamine (GlcN) units with a variable content (mostly 0 - 35%) of N-acetyl-D-glucosamine (GlcNAc) <cite>Yabuki1988 Boucher1992</cite>. Among the four types of links occurring between these two kinds of subunits in chitosan, all the enzymes examined for their cleavage specificity recognized productively the GlcN-GlcN links. Furthermore, the chitosanase from ''Bacillus circulans'' MH-K1 recognized also GlcN-GlcNAc links <cite>Mitsutomi1996</cite>, while the chitosanase from ''Streptomyces'' sp. N174 recognized the GlcNAc-GlcN links <cite>Fukamizo1995</cite>. |
| Line 56: | Line 56: | ||
#Yabuki1988 Yabuki, M., Uchiyama, A., Suzuki, K., Ando, A., Fujii, T. (1988) Purification and properties of chitosanase from ''Bacillus circulans'' MH-K1. Journal of General and Applied Microbiology 34:255-270. | #Yabuki1988 Yabuki, M., Uchiyama, A., Suzuki, K., Ando, A., Fujii, T. (1988) Purification and properties of chitosanase from ''Bacillus circulans'' MH-K1. Journal of General and Applied Microbiology 34:255-270. | ||
#Boucher1992 Boucher, I., Dupuy, A., Vidal, P., Neugebauer, W. A., Brzezinski, R. (1992) Purification and characterization of a chitosanase from ''Streptomyces'' N174. Applied Microbiology and Biotechnology 38:188-193. | #Boucher1992 Boucher, I., Dupuy, A., Vidal, P., Neugebauer, W. A., Brzezinski, R. (1992) Purification and characterization of a chitosanase from ''Streptomyces'' N174. Applied Microbiology and Biotechnology 38:188-193. | ||
| − | + | #Mitsutomi1996 Mitsutomi, M., Ueda, M., Arai, M., Ando, A., Watanabe, T. (1996) Action patterns of microbial chitinases and chitosanases on partially ''N''-acetylated chitosan. Chitin Enzymology, vol. 2, pp 273-284. | |
| + | #Fukamizo1995 Fukamizo, T., Honda, Y., Goto, S., Boucher, I., Brzezinski, R. (1995) Reaction mechanism of chitosanase from ''Streptomyces'' sp. N174. Biochemical Journal 311:377-383. | ||
</biblio> | </biblio> | ||
[[Category:Glycoside Hydrolase Families|GH046]] | [[Category:Glycoside Hydrolase Families|GH046]] | ||
Revision as of 11:20, 4 February 2010
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author: ^^^Ryszard Brzezinski^^^
- Responsible Curator: ^^^Ryszard Brzezinski^^^
| Glycoside Hydrolase Family GHnn | |
| Clan | GH-I |
| Mechanism | inverting |
| Active site residues | known |
| CAZy DB link | |
| http://www.cazy.org/fam/GH46.html | |
Substrate specificities
Glycoside hydrolases of family 46 are essentially endo-beta-1,4-chitosanases (EC 3.2.1.132) that hydrolyze various links in chitosan, a polymer of beta-1,4-linked D-glucosamine (GlcN) units with a variable content (mostly 0 - 35%) of N-acetyl-D-glucosamine (GlcNAc) [1, 2]. Among the four types of links occurring between these two kinds of subunits in chitosan, all the enzymes examined for their cleavage specificity recognized productively the GlcN-GlcN links. Furthermore, the chitosanase from Bacillus circulans MH-K1 recognized also GlcN-GlcNAc links [3], while the chitosanase from Streptomyces sp. N174 recognized the GlcNAc-GlcN links [4].
Kinetics and Mechanism
Content is to be added here.
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Content is to be added here.
Family Firsts
- First sterochemistry determination
- Cite some reference here, with a short (1-2 sentence) explanation (citation).
- First catalytic nucleophile identification
- Cite some reference here, with a short (1-2 sentence) explanation (citation).
- First general acid/base residue identification
- Cite some reference here, with a short (1-2 sentence) explanation (citation).
- First 3-D structure
- Cite some reference here, with a short (1-2 sentence) explanation (citation).
References
-
Yabuki, M., Uchiyama, A., Suzuki, K., Ando, A., Fujii, T. (1988) Purification and properties of chitosanase from Bacillus circulans MH-K1. Journal of General and Applied Microbiology 34:255-270.
-
Boucher, I., Dupuy, A., Vidal, P., Neugebauer, W. A., Brzezinski, R. (1992) Purification and characterization of a chitosanase from Streptomyces N174. Applied Microbiology and Biotechnology 38:188-193.
-
Mitsutomi, M., Ueda, M., Arai, M., Ando, A., Watanabe, T. (1996) Action patterns of microbial chitinases and chitosanases on partially N-acetylated chitosan. Chitin Enzymology, vol. 2, pp 273-284.
-
Fukamizo, T., Honda, Y., Goto, S., Boucher, I., Brzezinski, R. (1995) Reaction mechanism of chitosanase from Streptomyces sp. N174. Biochemical Journal 311:377-383.