Glycoside Hydrolase Family 74

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• Author: ^^^Katsuro Yaoi^^^ and ^^^Takuya Ishida^^^
• Responsible Curator: ^^^Katsuro Yaoi^^^

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Glycoside Hydrolase Family GH74
Clan	none, 7-fold β-propeller
Mechanism	inverting
Active site residues	known
CAZy DB link
http://www.cazy.org/GH74.html

Substrate specificities

Glycoside hydrolases of this family hydrolyze β-1,4-linkages of various glucans. With the exception of Cel74 from Thermotoga maritima, all biochemically characterized enzymes are specific toward xyloglucans and/or xyloglucan-oligosaccharides. Cel74 from Thermotoga maritima exhibits the highest activity on barley β-glucan, with relative activity of 20% toward xyloglucan. A wide diversity in the modes of action by GH-74 enzymes has been reported. "Oligoxyloglucan reducing end-specific cellobiohydrolase" from Geotrichum sp. M128, OXG-RCBH (EC 3.2.1.150) and "oligoxyloglucan reducing end-specific xyloglucanobiohydrolase (OREX)" from Emericella nidulans (formerly known as Aspergillus nidulans) are active on only xyloglucan oligosaccharides and have essentially no ability to degrade xyloglucan polysaccharides. They release oligosaccharides with two glucose units from non-reducing end of xyloglucan oligosaccharides [1]. On the other hand, GH-74 enzymes designated as xyloglucanase; xyloglucan specific endo-β-1,4-glucanases: XEG; and xyloglucan hydrolases: Xgh, (EC 3.2.1.151), exhibit endo-type activity on xyloglucan from tamarind seed, a readily available and well-investigated xyloglucan [2]. Many GH-74 xyloglucanases hydrolyze the glycosidic linkage of unbranched glucose residues, but several members including OXG-RCBH, OREX, and Cel74A from Hypocrea jecorina (formerly known as Trichoderma reesei) accommodate side-chain xylose residues at subsite -1 [3] of the active site.

Kinetics and Mechanism

Family 74 enzymes are inverting enzymes, as shown by NMR analysis on Xeg74 from Thermobifida fusca [4] and XEG from Geotrichum sp. M128 [5].

Catalytic Residues

Crystal structure of OXG-RCBH demonstrated that Asp35 and Asp465 are located in the middle of the binding cleft, and its crucial roles in hydrolytic activity were experimentally confirmed by using site-directed mutagenesis [6]. The corresponding Asp residues in Clostridium thermocellum are nicely located between subsites -1 and +1 in the complex structure [7].

Three-dimensional structures

Over all structures of GH-74 enzymes consist of a tandem repeat of two seven-bladed β-propeller domains. The two domains form a open cleft substrate binding site at the interface. The catalytic residues are located in the middle of this cleft. One side of the binding cleft of OXG-RCBH is blocked by a so-called 'exo-loop' which is found only in exo-acting enzymes in this family. A crystal structure of a complex with xyloglucan oligo-saccharides elucidated the interaction with the side-chains of the substrate by these enzymes [6, 7].

Family Firsts

First stereochemistry determination

Xeg74 from Thermobifida fusca by ¹H-NMR [4].

First gene cloning

EglC from Aspergillus niger [8].

First general acid residue identification

OXG-RCBH from Geotrichum sp. M128 [6].

First general base residue identification

OXG-RCBH from Geotrichum sp. M128 [6].

First 3-D structure

OXG-RCBH from Geotrichum sp. M128 [6].

References

1. Yaoi K and Mitsuishi Y. (2002). Purification, characterization, cloning, and expression of a novel xyloglucan-specific glycosidase, oligoxyloglucan reducing end-specific cellobiohydrolase. J Biol Chem. 2002;277(50):48276-81. DOI:10.1074/jbc.M208443200 |
2. York WS, Harvey LK, Guillen R, Albersheim P, and Darvill AG. (1993). Structural analysis of tamarind seed xyloglucan oligosaccharides using beta-galactosidase digestion and spectroscopic methods. Carbohydr Res. 1993;248:285-301. DOI:10.1016/0008-6215(93)84135-s |
3. Davies GJ, Wilson KS, and Henrissat B. (1997). Nomenclature for sugar-binding subsites in glycosyl hydrolases. Biochem J. 1997;321 ( Pt 2)(Pt 2):557-9. DOI:10.1042/bj3210557 |
4. Irwin DC, Cheng M, Xiang B, Rose JK, and Wilson DB. (2003). Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca. Eur J Biochem. 2003;270(14):3083-91. DOI:10.1046/j.1432-1033.2003.03695.x |
5. Yaoi K and Mitsuishi Y. (2004). Purification, characterization, cDNA cloning, and expression of a xyloglucan endoglucanase from Geotrichum sp. M128. FEBS Lett. 2004;560(1-3):45-50. DOI:10.1016/S0014-5793(04)00068-7 |
6. Yaoi K, Kondo H, Noro N, Suzuki M, Tsuda S, and Mitsuishi Y. (2004). Tandem repeat of a seven-bladed beta-propeller domain in oligoxyloglucan reducing-end-specific cellobiohydrolase. Structure. 2004;12(7):1209-17. DOI:10.1016/j.str.2004.04.020 |
7. Martinez-Fleites C, Guerreiro CI, Baumann MJ, Taylor EJ, Prates JA, Ferreira LM, Fontes CM, Brumer H, and Davies GJ. (2006). Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation. J Biol Chem. 2006;281(34):24922-33. DOI:10.1074/jbc.M603583200 |
8. Hasper AA, Dekkers E, van Mil M, van de Vondervoort PJ, and de Graaff LH. (2002). EglC, a new endoglucanase from Aspergillus niger with major activity towards xyloglucan. Appl Environ Microbiol. 2002;68(4):1556-60. DOI:10.1128/AEM.68.4.1556-1560.2002 |