Glycoside Hydrolase Family 75

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• Author:
• Responsible Curator: ^^^Ryszard Brzezinski^^^

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Glycoside Hydrolase Family GH75
Clan	GH-x
Mechanism	inverting
Active site residues	known/not known
CAZy DB link
https://www.cazy.org/GH75.html

Substrate specificities

Glycoside hydrolases of family 75 include both eukaryotic (essentially fungal) and prokaryotic proteins. They have so far been characterized only from fimanetous fungi. They are primarily of beta-1,4-chitosanases with endo-splitting activity [1, 2].

Kinetics and Mechanism

Family GH46 enzymes utilize an inverting mechanism as shown by NMR [3].

Catalytic Residues

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Three-dimensional structures

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This is an example of how to make references to a journal article [4]. (See the References section below). Multiple references can go in the same place like this [4, 5]. You can even cite books using just the ISBN [6]. References that are not in PubMed can be typed in by hand [7].

Family Firsts

First stereochemistry determination

Cite some reference here, with a short (1-2 sentence) explanation [4].

First catalytic nucleophile identification

Cite some reference here, with a short (1-2 sentence) explanation [7].

First general acid/base residue identification

Cite some reference here, with a short (1-2 sentence) explanation [5].

First 3-D structure

Cite some reference here, with a short (1-2 sentence) explanation [6].

References

1. Shimosaka M, Nogawa M, Ohno Y, and Okazaki M. Chitosanase from the pathogenic fungus, Fusarium solani f.sp. phaseoli - purification and some properties. Biosci. Biotech. Biochem. 57, 231-235.

2. Cheng CY and Li YK. (2000). An Aspergillus chitosanase with potential for large-scale preparation of chitosan oligosaccharides. Biotechnol Appl Biochem. 2000;32(3):197-203. DOI:10.1042/ba20000063 |
4. Comfort DA, Bobrov KS, Ivanen DR, Shabalin KA, Harris JM, Kulminskaya AA, Brumer H, and Kelly RM. (2007). Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Biochemistry. 2007;46(11):3319-30. DOI:10.1021/bi061521n |
5. He S and Withers SG. (1997). Assignment of sweet almond beta-glucosidase as a family 1 glycosidase and identification of its active site nucleophile. J Biol Chem. 1997;272(40):24864-7. DOI:10.1074/jbc.272.40.24864 |

7. Sinnott, M.L. (1990) Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90, 1171-1202. DOI: 10.1021/cr00105a006