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Difference between revisions of "Glycoside Hydrolase Family 121"
| Line 32: | Line 32: | ||
== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
| − | HypBA2 is a retaining enzyme. The stereochemical course of the reaction was shown by transglycosylation activity toward | + | HypBA2 is a retaining enzyme. The stereochemical course of the reaction was shown by transglycosylation activity toward methanol, and following <sup>1</sup>H-NMR and <sup>13</sup>C-NMR analysis of the transglycosylation product (Ara''f''β1-2Ara''f''β-Me). |
== Catalytic Residues == | == Catalytic Residues == | ||
Revision as of 23:45, 4 November 2012
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author: ^^^Kiyotaka Fujita^^^
- Responsible Curator: ^^^Shinya Fushinobu^^^
| Glycoside Hydrolase Family GH121 | |
| Clan | GH-x |
| Mechanism | retaining |
| Active site residues | not known |
| CAZy DB link | |
| https://www.cazy.org/GH121.html | |
Substrate specificities
This family of glycoside hydrolases contains β-L-arabinobiosidase, which was recently established for HypBA2 from Bifidobacterium longum JCM 1217[1]. HypBA2 liberated Arafβ1-2Araf (β-Ara2) from unmodified Arafβ1-2Arafβ1-2Arafβ-hydroxyproline (Ara3-Hyp) but not Arafα1-3Arafβ1-2Arafβ1-2Arafβ-Hyp (Ara4-Hyp) or Arafβ1-2Arafβ-Hyp (Ara2-Hyp). HypBA2 directly liberated β-Ara2 from hydroxyproline-rich glycoproteins (HRGPs) such as carrot extensin and potato lectin. The family members are only found from Bacterial genomes.
Kinetics and Mechanism
HypBA2 is a retaining enzyme. The stereochemical course of the reaction was shown by transglycosylation activity toward methanol, and following 1H-NMR and 13C-NMR analysis of the transglycosylation product (Arafβ1-2Arafβ-Me).
Catalytic Residues
Not known.
Three-dimensional structures
Not known.
Family Firsts
- First stereochemistry determination
- This was determined with HypBA2 enzyme by measurement of glycosyl transfer reactions to methanol and the 1H-NMR and 13C-NMR spectra.
- First catalytic nucleophile identification
- No experimental proof.
- First general acid/base residue identification
- No experimental proof.
- First 3-D structure
- Not known.