Polysaccharide Lyase Family 30

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• Author: Marie-Line Garron
• Responsible Curator: Marie-Line Garron

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Polysaccharide Lyase Family PL30
3D Structure
Mechanism
Charge neutraliser
Active site residues
CAZy DB link
https://www.cazy.org/PL30.html

Substrate specificities

PL30 is a small family (38 sequences), exclusively bacterial belonging to the phylum Bacteroidetes (PL30) [1]. The founding member of family 30, and the only one characterized, belongs to Bacteroides cellulosilyticus WH2 (BcellWH2_02524, WP_029426181.1). The activity was measured against hyaluronic acid by following the absorbance at 232nm. The increase in absorbance confirms that BcellWH2_02524 is a hyaluronan lyase (4.2.2.1), probably using the by b-elimination process like all PL families [2].

Kinetics and Mechanism

Not Determined

Catalytic Residues

Not Determined

Three-dimensional structures

Unsolved

Family Firsts

First stereochemistry determination

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First catalytic nucleophile identification

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First general acid/base residue identification

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First 3-D structure

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References

1. Drula E, Garron ML, Dogan S, Lombard V, Henrissat B, and Terrapon N. (2022). The carbohydrate-active enzyme database: functions and literature. Nucleic Acids Res. 2022;50(D1):D571-D577. DOI:10.1093/nar/gkab1045 |
2. Helbert W, Poulet L, Drouillard S, Mathieu S, Loiodice M, Couturier M, Lombard V, Terrapon N, Turchetto J, Vincentelli R, and Henrissat B. (2019). Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space. Proc Natl Acad Sci U S A. 2019;116(13):6063-6068. DOI:10.1073/pnas.1815791116 |