User:Priscila Giuseppe

Priscila Giuseppe earned a B.Sc. in Biological Sciences from the State University of Campinas and a PhD in Protein X-ray Crystallography from the Graduate Program in Genetics and Molecular Biology at the same university. Her doctoral research was conducted at CNPEM (Brazil) and Synchrotron Soleil (France), under the supervision of Beatriz Guimarães, and at the Institut Pasteur (France) under the supervision of Mathieu Picardeau. In 2011, she joined the group of Mario Murakami, as a research associate at the Brazilian Biosciences National Laboratory (LNBio-CNPEM), contributing on several studies on the molecular mechanisms that drive protein function and biocatalysis. Since 2020 she has been a Principal Investigator at the [ https://lnbr.cnpem.br/ Brazilian Biorenewables National Laboratory], leading a research group focused on the study and development of biocatalytic strategies for lignin valorization. In the glycobiology field, she has contributed to structural and functional studies related to members of the following CAZy families:

• GH1 HiBG [1] and EaBglA [2]

• GH3 XacXyl3A, XacXyl3B, XacBgl3A, XacBgl3B, XacBgl3C [3]

• GH5 subfamily 18 BlMan5B [4]

• GH8 XacCel8 [5]

• GH31 XacXyl31 [3] and Bl_Glc31 [6]

• GH35 XacGalD [3]

• GH38 Bl_Man38A, Bl_Man38B, Bl_Man38C [6]

• GH51 XacAbf51 [7]

• GH74 XacXeg74 [3]

• GH85 Bl_Endo85 [6]

• GH95 XacAfc95 [3]

• GH125 Bl_Man125 [6]

• CE20 XacXaeA [3]

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1. de Giuseppe PO, Souza Tde A, Souza FH, Zanphorlin LM, Machado CB, Ward RJ, Jorge JA, Furriel Rdos P, and Murakami MT. (2014). Structural basis for glucose tolerance in GH1 β-glucosidases. Acta Crystallogr D Biol Crystallogr. 2014;70(Pt 6):1631-9. DOI:10.1107/S1399004714006920 |
2. Zanphorlin LM, de Giuseppe PO, Honorato RV, Tonoli CC, Fattori J, Crespim E, de Oliveira PS, Ruller R, and Murakami MT. (2016). Oligomerization as a strategy for cold adaptation: Structure and dynamics of the GH1 β-glucosidase from Exiguobacterium antarcticum B7. Sci Rep. 2016;6:23776. DOI:10.1038/srep23776 |
3. Vieira PS, Bonfim IM, Araujo EA, Melo RR, Lima AR, Fessel MR, Paixão DAA, Persinoti GF, Rocco SA, Lima TB, Pirolla RAS, Morais MAB, Correa JBL, Zanphorlin LM, Diogo JA, Lima EA, Grandis A, Buckeridge MS, Gozzo FC, Benedetti CE, Polikarpov I, Giuseppe PO, and Murakami MT. (2021). Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors. Nat Commun. 2021;12(1):4049. DOI:10.1038/s41467-021-24277-4 |
4. Cordeiro RL, Pirolla RAS, Persinoti GF, Gozzo FC, de Giuseppe PO, and Murakami MT. (2019). N-glycan Utilization by Bifidobacterium Gut Symbionts Involves a Specialist β-Mannosidase. J Mol Biol. 2019;431(4):732-747. DOI:10.1016/j.jmb.2018.12.017 |
5. de Melo RR, de Lima EA, Persinoti GF, Vieira PS, de Sousa AS, Zanphorlin LM, de Giuseppe PO, Ruller R, and Murakami MT. (2021). Identification of a cold-adapted and metal-stimulated β-1,4-glucanase with potential use in the extraction of bioactive compounds from plants. Int J Biol Macromol. 2021;166:190-199. DOI:10.1016/j.ijbiomac.2020.10.137 |
6. Cordeiro RL, Santos CR, Domingues MN, Lima TB, Pirolla RAS, Morais MAB, Colombari FM, Miyamoto RY, Persinoti GF, Borges AC, de Farias MA, Stoffel F, Li C, Gozzo FC, van Heel M, Guerin ME, Sundberg EJ, Wang LX, Portugal RV, Giuseppe PO, and Murakami MT. (2023). Mechanism of high-mannose N-glycan breakdown and metabolism by Bifidobacterium longum. Nat Chem Biol. 2023;19(2):218-229. DOI:10.1038/s41589-022-01202-4 |
7. Dos Santos CR, de Giuseppe PO, de Souza FHM, Zanphorlin LM, Domingues MN, Pirolla RAS, Honorato RV, Tonoli CCC, de Morais MAB, de Matos Martins VP, Fonseca LM, Büchli F, de Oliveira PSL, Gozzo FC, and Murakami MT. (2018). The mechanism by which a distinguishing arabinofuranosidase can cope with internal di-substitutions in arabinoxylans. Biotechnol Biofuels. 2018;11:223. DOI:10.1186/s13068-018-1212-y |