User:Masahiro Nakajima

Masahiro Nakajima received his Ph.D. from the Graduate School of Agricultural and Life Science, The University of Tokyo in 2006. He joined the group of Dr. Motomitsu Kitaoka as a postdoctoral fellow (2006–2010). He moved to Iwate Biotechnology Research Center as a researcher (2010–2012). He was an assistant professor in Taguchi Laboratory (2012–2020) and is currently an associate professor in his own laboratory (2020-) at Department of Applied Biological Science, Tokyo University of Science. His research currently focuses on structures and functions of carbohydrate-active enzymes acting on unique sugar chains such as β-1,2-glucan. He acts as a Responsible Curator of Glycoside Hydrolase Families GH144, GH162, GH186 and GH189 and also created clan GH-S. He determined the functions and/or crystal structures of

• GH3 β-Glucosidases [1, 2, 3]
• GH16 β-1,3-Glucanase [4]
• GH35 β-1,2-Glucosyltransglycosylase [5] (new EC number)
• GH38 α-Mannosidase [6]
• GH57 4-α-Glucanotransferase [7]
• GH94 1,2-β-Oligoglucan phosphorylases [8, 9] (new EC number)
• GH112 D-Galactosyl-β-1,4-L-rhamnose phosphorylase [10] (new EC number) and β-1,3-galactosyl-N-acetylhexosamine phosphorylases [10, 11, 12, 13]
• GH144 Bacterial β-1,2-glucanases [14] (family created) and sophorosylhydrolase [15] (new EC number)
• GH162 Fungal β-1,2-glucanase [16] (family created) (clan GH-S created)
• GH186 E. coli β-1,2-glucanase [17] (family created)
• GH189 Cyclic β-1,2-glucan synthase (transglycosylase domain) [18] (family created) (new EC number)

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10. Nakajima M, Nishimoto M, and Kitaoka M. (2009). Characterization of three beta-galactoside phosphorylases from Clostridium phytofermentans: discovery of d-galactosyl-beta1->4-l-rhamnose phosphorylase. J Biol Chem. 2009;284(29):19220-7. DOI:10.1074/jbc.M109.007666 |
12. Nakajima M and Kitaoka M. (2008). Identification of lacto-N-Biose I phosphorylase from Vibrio vulnificus CMCP6. Appl Environ Microbiol. 2008;74(20):6333-7. DOI:10.1128/AEM.02846-07 |
13. Nakajima M, Nihira T, Nishimoto M, and Kitaoka M. (2008). Identification of galacto-N-biose phosphorylase from Clostridium perfringens ATCC13124. Appl Microbiol Biotechnol. 2008;78(3):465-71. DOI:10.1007/s00253-007-1319-8 |
14. Abe K, Nakajima M, Yamashita T, Matsunaga H, Kamisuki S, Nihira T, Takahashi Y, Sugimoto N, Miyanaga A, Nakai H, Arakawa T, Fushinobu S, and Taguchi H. (2017). Biochemical and structural analyses of a bacterial endo-β-1,2-glucanase reveal a new glycoside hydrolase family. J Biol Chem. 2017;292(18):7487-7506. DOI:10.1074/jbc.M116.762724 |
16. Tanaka N, Nakajima M, Narukawa-Nara M, Matsunaga H, Kamisuki S, Aramasa H, Takahashi Y, Sugimoto N, Abe K, Terada T, Miyanaga A, Yamashita T, Sugawara F, Kamakura T, Komba S, Nakai H, and Taguchi H. (2019). Identification, characterization, and structural analyses of a fungal endo-β-1,2-glucanase reveal a new glycoside hydrolase family. J Biol Chem. 2019;294(19):7942-7965. DOI:10.1074/jbc.RA118.007087 |
18. Tanaka N, Saito R, Kobayashi K, Nakai H, Kamo S, Kuramochi K, Taguchi H, Nakajima M, and Masaike T. (2024). Functional and structural analysis of a cyclization domain in a cyclic β-1,2-glucan synthase. Appl Microbiol Biotechnol. 2024;108(1):187. DOI:10.1007/s00253-024-13013-9 |