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User:Sei Motouchi

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I am a Ph.D. candidate at Tokyo University of Science. My research interests concern structure and function of new CAZymes, especially β-1,2-glucan-related enzymes responsible for enabling interactions between organisms. I contributed to the biochemical identification and/or structural determination of:

  • GH186 Escherichia coli endo β-1,2-glucanase (EcOpgD) (biochemical identification, determination of Michaelis complex and Suggestion of unique reaction mechanism) Family first [1].
  • GH186 Escherichia coli endo β-1,2-glucanase (EcOpgG) (biochemical identification and determination of Michaelis complex) [1].
  • GH186 Xanthomonas campestris pv. campestris α-1,6-cyclized β-1,2-glucohexadecaose synthase (XccOpgD) (biochemical identification, determination of Michaelis complex and Suggestion of reaction mechanism) First-identified anomer-inverting transglycosylase [2].




  1. Motouchi S, Kobayashi K, Nakai H, and Nakajima M. (2023). Identification of enzymatic functions of osmo-regulated periplasmic glucan biosynthesis proteins from Escherichia coli reveals a novel glycoside hydrolase family. Commun Biol. 2023;6(1):961. DOI:10.1038/s42003-023-05336-6 | PubMed ID:37735577 [Motouchi2023]
  2. Motouchi S, Komba S, Nakai H, and Nakajima M. (2024). Discovery of Anomer-Inverting Transglycosylase: Cyclic Glucohexadecaose-Producing Enzyme from Xanthomonas, a Phytopathogen. J Am Chem Soc. 2024;146(26):17738-17746. DOI:10.1021/jacs.4c02579 | PubMed ID:38957137 [Motouchi2024]

All Medline abstracts: PubMed