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Polysaccharide Lyase Family 30
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| Polysaccharide Lyase Family PL30 | |
| 3D Structure | |
| Mechanism | |
| Charge neutraliser | |
| Active site residues | |
| CAZy DB link | |
| https://www.cazy.org/PL30.html | |
Substrate specificities
PL30 is a small family (38 sequences), exclusively bacterial belonging to the phylum Bacteroidetes (PL30) [1]. The founding member of family 30, and the only one characterized, belongs to Bacteroides cellulosilyticus WH2 (BcellWH2_02524, WP_029426181.1). The activity was measured against hyaluronic acid by following the absorbance at 232nm. The increase in absorbance confirms that BcellWH2_02524 is a hyaluronan lyase (4.2.2.1), probably using the by b-elimination process like all PL families [2].
Kinetics and Mechanism
Not Determined
Catalytic Residues
Not Determined
Three-dimensional structures
Unsolved
Family Firsts
- First description of catalytic activity
- Hyaluronan lyase was the first activity discovered for the family PL30 [2].
- First catalytic base identification
- Content is to be added here.
- First catalytic acid identification
- Content is to be added here.
- First 3-D structure
- Content is to be added here.
References
- Drula E, Garron ML, Dogan S, Lombard V, Henrissat B, and Terrapon N. (2022). The carbohydrate-active enzyme database: functions and literature. Nucleic Acids Res. 2022;50(D1):D571-D577. DOI:10.1093/nar/gkab1045 |
- Helbert W, Poulet L, Drouillard S, Mathieu S, Loiodice M, Couturier M, Lombard V, Terrapon N, Turchetto J, Vincentelli R, and Henrissat B. (2019). Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space. Proc Natl Acad Sci U S A. 2019;116(13):6063-6068. DOI:10.1073/pnas.1815791116 |