Carbohydrate Binding Module Family 47

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• Author: Wenwen Tao
• Responsible Curator: Yaoguang Chang

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CAZy DB link

http://www.cazy.org/CBM47.html

Ligand specificities

The C-terminal triplet fucose-binding module (SpX-1.2.3) of protein toxin SpGH98, originating from the fucose utilization operon in Streptococcus pneumoniae, has been firstly designated as CBM47 [1]. Anguilla anguilla agglutinin (AAA), derived from the European eel, which was characterized earlier and initially classified as an F-type lectin [2], was also included in the CBM47 family. Several other members of CBM47 family, such as MsaFBP32 [3], LLY^lec [4] and its mutant LLY^lecY62H [5], have been confirmed to possess the F-type lectin fold. The aforementioned proteins are all capable of binding to polysaccharides containing fucose, often trisaccharides or smaller, such as AAA, and a portion of CBM47 members has been demonstrated to bind to polysaccharides with galactose [2]. Both of these sugars are ubiquitous in glycoproteins and glycolipids on the cell surface, playing crucial roles in cellular bioactivity and functions. Furthermore, the CBM47 family exhibits specific binding to Lewis blood group oligosaccharides, which are characterized by fucosylation modifications. For instance, LLY^lec binds to both Lewis y (Le^y) antigen (type 1 antigen) and Lewis b (Le^b) antigen (type 2 antigen) [4], whereas AAA displays a preference for binding to the Le^y antigen [2]. Consequently, the CBM47 family holds potential applications in immune recognition and disease diagnosis. Besides, a novel CBM47 domain was discovered from the marine bacterium Wenyingzhuangia fucanilytica, which is appended to the GH168 family sequence. The CBM exhibited a specific binding capacity to sulfated fucans with the backbone composed of 1,3-α-L-fucopyranose residues [6].

Structural Features

The core structures of the CBM47 family exhibit remarkable similarity, adopting an eight-stranded β-sandwich fold, which is comprised of a five-stranded anti-parallel β-sheet on one side and a three-stranded anti-parallel β-sheet on the other [1, 2, 3, 4, 5]. Additionally, CBM47 typically exists as a dimer or trimer under physiological conditions, with Ca²⁺ playing a pivotal role in maintaining its conformational stability. The CBM47 family recognizes and binds polysaccharides through shallow grooves located within their complementarity-determining regions (CDRs), and these variable loops have been shown to exhibit a high degree of sequence and conformational variability among different CBM47s, enabling them to recognize diverse ligands. For instance, LLY^lec and SpX-1 reveal smaller differences among their loops, permitting them to bind to a wide array of Lewis blood group oligosaccharides [1, 4]. While AAA features a particularly longer and rigid loop, which may account for its preference to form complexes with type 1 antigens while lacking affinity for type 2 antigens [2]. Polysaccharide binding via loop regions typically tends to be of shorter length.

Functionalities

Content in this section should include, in paragraph form, a description of:

• Functional role of CBM: Describe common functional roles such as targeting, disruptive, anchoring, proximity/position on substrate.
• Most Common Associated Modules: 1. Glycoside Hydrolase Activity; 2. Additional Associated Modules (other CBM, FNIII, cohesin, dockerins, expansins, etc.)
• Novel Applications: Include here if CBM has been used to modify another enzyme, or if a CBM was used to label plant/mammalian tissues? Etc.

Family Firsts

First Identified

Insert archetype here, possibly including very brief synopsis.

First Structural Characterization

Insert archetype here, possibly including very brief synopsis.

References

1. Boraston AB, Wang D, and Burke RD. (2006). Blood group antigen recognition by a Streptococcus pneumoniae virulence factor. J Biol Chem. 2006;281(46):35263-71. DOI:10.1074/jbc.M607620200 |
2. Bianchet MA, Odom EW, Vasta GR, and Amzel LM. (2002). A novel fucose recognition fold involved in innate immunity. Nat Struct Biol. 2002;9(8):628-34. DOI:10.1038/nsb817 |
3. Bianchet MA, Odom EW, Vasta GR, and Amzel LM. (2010). Structure and specificity of a binary tandem domain F-lectin from striped bass (Morone saxatilis). J Mol Biol. 2010;401(2):239-52. DOI:10.1016/j.jmb.2010.06.018 |
4. Feil SC, Lawrence S, Mulhern TD, Holien JK, Hotze EM, Farrand S, Tweten RK, and Parker MW. (2012). Structure of the lectin regulatory domain of the cholesterol-dependent cytolysin lectinolysin reveals the basis for its lewis antigen specificity. Structure. 2012;20(2):248-58. DOI:10.1016/j.str.2011.11.017 |
5. Lawrence SL, Feil SC, Holien JK, Kuiper MJ, Doughty L, Dolezal O, Mulhern TD, Tweten RK, and Parker MW. (2012). Manipulating the Lewis antigen specificity of the cholesterol-dependent cytolysin lectinolysin. Front Immunol. 2012;3:330. DOI:10.3389/fimmu.2012.00330 |
6. Mei X, Chang Y, Shen J, Zhang Y, Chen G, Liu Y, and Xue C. (2022). Characterization of a sulfated fucan-specific carbohydrate-binding module: A promising tool for investigating sulfated fucans. Carbohydr Polym. 2022;277:118748. DOI:10.1016/j.carbpol.2021.118748 |