Glycoside Hydrolase Family 12

This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.

• Author: ^^^Mats Sandgren^^^
• Responsible Curator: ^^^Mats Sandgren^^^

---

Glycoside Hydrolase Family GHnn
Clan	GH-C
Mechanism	retaining
Active site residues	known
CAZy DB link
http://www.cazy.org/fam/GH12.html

Substrate specificities

The substrate specificities found among the glycoside hydrolases of family 12 are: endo-β-1,4-glucanase (EC 3.2.1.4), xyloglucan hydrolase (EC 3.2.1.151), endo-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan endo-transglycosylase (EC 2.4.1.207),.

Kinetics and Mechanism

GH Family 12 enzymes are retaining enzymes, as first shown by NMR studdies [1] of endoglucanase 3 from Humicola insolens, and follow a classical Koshland double-displacement mechanism. No detailed studies involving both steady state and pre-steady state kinetic have jet been reported for this family.

Catalytic Residues

Content is to be added here.

Three-dimensional structures

Content is to be added here.

Family Firsts

First sterochemistry determination

Cite some reference here, with a short (1-2 sentence) explanation [2].

First catalytic nucleophile identification

Cite some reference here, with a short (1-2 sentence) explanation [3].

First general acid/base residue identification

Cite some reference here, with a short (1-2 sentence) explanation [4].

First 3-D structure

Cite some reference here, with a short (1-2 sentence) explanation [5].

References

1. Schou C, Rasmussen G, Kaltoft MB, Henrissat B, and Schülein M. (1993). Stereochemistry, specificity and kinetics of the hydrolysis of reduced cellodextrins by nine cellulases. Eur J Biochem. 1993;217(3):947-53. DOI:10.1111/j.1432-1033.1993.tb18325.x |