User:Mario Murakami

Mario Murakami is the scientific director of the Brazilian Biorenewables National Laboratory (since 2018) and former coordinator of the macromolecular crystallography village at the Brazilian National Center for Research in Energy and Materials (2010-2017). He obtained Ph.D. degree in molecular biophysics (2006) from the State University of São Paulo with a split Ph.D. at the University of Hamburg and German Electron Synchrotron DESY. He worked with the structural elucidation of macromolecular complexes involved in the inhibition and activation of enzymes during his post-docs at UNESP and Rutgers University. His current research interests encompass the discovery and mechanistic understanding of CAZymes and the genetic engineering of filamentous fungi for enzyme production. He has contributed to structure and function studies of CAZymes from families GH1 [1, 2, 3, 4, 5, 6], GH2 [7], GH5 [8, 9, 10, 11, 12, 13], GH7 [14], GH8 [15], GH10 [16, 17, 18], GH11 [19, 20, 21], GH12 [22, 23, 24], GH16 [25, 26], GH26 [27], GH39 [28, 29], GH42 [30], GH43 [31, 32, 33], GH45 [34], GH51 [35, 36], GH54 [37], GH57 [38], GH128 [39] and AA9 [40]. Recently, his group rationally engineered a publicly available strain (Trichoderma reesei RUT-C30), which can secrete more than 80 g/L of proteins, mostly CAZymes, using a low-cost and byproduct-based bioprocess [41].

---

Selected publications

Santos CR, Costa PACR, Vieira PS, Gonzalez SET, Correa TLR, Lima EA, Mandelli F, Pirolla RAS, Domingues MN, Cabral L, Martins MP, Cordeiro RL, Junior AT, Souza BP, Prates ÉT, Gozzo FC, Persinoti GF, Skaf MS, and Murakami MT. (2020) Structural insights into β-1,3-glucan cleavage by a glycoside hydrolase family. Nat Chem Biol. DOI:10.1038/s41589-020-0554-5 HubMed

Fonseca LM, Parreiras LS, and Murakami MT. (2020) Rational engineering of the Trichoderma reesei RUT-C30 strain into an industrially relevant platform for cellulase production. Biotechnol Biofuels. 13, 93. DOI:10.1186/s13068-020-01732-w HubMed

Mandelli F, de Morais MAB, de Lima EA, Oliveira L, Persinoti GF, and Murakami MT. (2020) Spatially remote motifs cooperatively affect substrate preference of a ruminal GH26-type endo-β-1,4-mannanase. J Biol Chem. 295, 5012-5021. DOI:10.1074/jbc.RA120.012583 HubMed

Cordeiro RL, Pirolla RAS, Persinoti GF, Gozzo FC, de Giuseppe PO, and Murakami MT. (2019) N-glycan Utilization by Bifidobacterium Gut Symbionts Involves a Specialist β-Mannosidase. J Mol Biol. 431, 732-747. DOI:10.1016/j.jmb.2018.12.017 HubMed

Corrêa TLR, Júnior AT, Wolf LD, Buckeridge MS, Dos Santos LV, and Murakami MT. (2019) An actinobacteria lytic polysaccharide monooxygenase acts on both cellulose and xylan to boost biomass saccharification. Biotechnol Biofuels. 12, 117. DOI:10.1186/s13068-019-1449-0 HubMed

Domingues MN, Souza FHM, Vieira PS, de Morais MAB, Zanphorlin LM, Dos Santos CR, Pirolla RAS, Honorato RV, de Oliveira PSL, Gozzo FC, and Murakami MT. (2018) Structural basis of exo-β-mannanase activity in the GH2 family. J Biol Chem. 293, 13636-13649. DOI:10.1074/jbc.RA118.002374 HubMed

Dos Santos CR, de Giuseppe PO, de Souza FHM, Zanphorlin LM, Domingues MN, Pirolla RAS, Honorato RV, Tonoli CCC, de Morais MAB, de Matos Martins VP, Fonseca LM, Büchli F, de Oliveira PSL, Gozzo FC, and Murakami MT. (2018) The mechanism by which a distinguishing arabinofuranosidase can cope with internal di-substitutions in arabinoxylans. Biotechnol Biofuels. 11, 223. DOI:10.1186/s13068-018-1212-y HubMed

Dos Santos CR, Cordeiro RL, Wong DW, and Murakami MT. (2015) Structural basis for xyloglucan specificity and α-d-Xylp(1 → 6)-D-Glcp recognition at the -1 subsite within the GH5 family. Biochemistry. 54, 1930-42. DOI:10.1021/acs.biochem.5b00011 HubMed

Santos CR, Hoffmam ZB, de Matos Martins VP, Zanphorlin LM, de Paula Assis LH, Honorato RV, Lopes de Oliveira PS, Ruller R, and Murakami MT. (2014) Molecular mechanisms associated with xylan degradation by Xanthomonas plant pathogens. J Biol Chem. 289, 32186-200. DOI:10.1074/jbc.M114.605105 HubMed

Santos CR, Polo CC, Costa MC, Nascimento AF, Meza AN, Cota J, Hoffmam ZB, Honorato RV, Oliveira PS, Goldman GH, Gilbert HJ, Prade RA, Ruller R, Squina FM, Wong DW, and Murakami MT. (2014) Mechanistic strategies for catalysis adopted by evolutionary distinct family 43 arabinanases. J Biol Chem. 289, 7362-73. DOI:10.1074/jbc.M113.537167 HubMed

de Giuseppe PO, Souza Tde A, Souza FH, Zanphorlin LM, Machado CB, Ward RJ, Jorge JA, Furriel Rdos P, and Murakami MT. (2014) Structural basis for glucose tolerance in GH1 β-glucosidases. Acta Crystallogr D Biol Crystallogr. 70, 1631-9. DOI:10.1107/S1399004714006920 HubMed

---

Solved structures

---

References

Error fetching PMID 24914974:
Error fetching PMID 26475230:
Error fetching PMID 27006690:
Error fetching PMID 27029646:
Error fetching PMID 29454992:
Error fetching PMID 30894609:
Error fetching PMID 29997257:
Error fetching PMID 22155669:
Error fetching PMID 21880019:
Error fetching PMID 24358302:
Error fetching PMID 25714929:
Error fetching PMID 27334041:
Error fetching PMID 30641082:
Error fetching PMID 22494694:
Error fetching PMID 28448629:
Error fetching PMID 21070746:
Error fetching PMID 23922891:
Error fetching PMID 25266726:
Error fetching PMID 16289057:
Error fetching PMID 22006920:
Error fetching PMID 26923808:
Error fetching PMID 22230786:
Error fetching PMID 25605422:
Error fetching PMID 28088615:
Error fetching PMID 21352806:
Error fetching PMID 23459129:
Error fetching PMID 32139511:
Error fetching PMID 22993088:
Error fetching PMID 32500063:
Error fetching PMID 27685756:
Error fetching PMID 24469445:
Error fetching PMID 25640722:
Error fetching PMID 30556897:
Error fetching PMID 30396396:
Error fetching PMID 21796714:
Error fetching PMID 30127853:
Error fetching PMID 22750495:
Error fetching PMID 21104698:
Error fetching PMID 32451508:
Error fetching PMID 31168322:
Error fetching PMID 32461765: