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Glycoside Hydrolase Family 68
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- Author: ^^^Tirso Pons^^^ and ^^^Wim Van den Ende^^^
- Responsible Curator: ^^^Wim Van den Ende^^^
| Glycoside Hydrolase Family GH68 | |
| Clan | GH-J |
| Mechanism | retaining |
| Active site residues | known |
| CAZy DB link | |
| http://www.cazy.org/fam/GH68.html | |
Substrate specificities
Glycoside hydrolase family GH68 contains enzymes that hydrolyze fructose containing polysaccharides such as levansucrase (EC 2.4.1.10); β-fructofuranosidase (EC 3.2.1.26); and inulosucrase (EC 2.4.1.9)
Kinetics and Mechanism
Content is to be added here.
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Currently, only two different three dimensional structures of family GH68 enzymes have been solved so far. The first crystal structure was reported for the bacterial levansucrase (SacB) from Bacillus subtilis subsp. subtilis str. 168 [1]. The second one corresponds to levansucrase (LdsA) from Gluconacetobacter diazotrophicus SRT4 [2].
Family Firsts
- First stereochemistry determination
- Cite some reference here, with a short (1-2 sentence) explanation [3].
- First catalytic nucleophile identification
- Cite some reference here, with a short (1-2 sentence) explanation [4].
- First general acid/base residue identification
- Cite some reference here, with a short (1-2 sentence) explanation [5].
- First 3-D structure
- Cite some reference here, with a short (1-2 sentence) explanation [6].
References
You can even cite books using just the ISBN [6]. References that are not in PubMed can be typed in by hand [4].
- Meng G and Fütterer K. (2003). Structural framework of fructosyl transfer in Bacillus subtilis levansucrase. Nat Struct Biol. 2003;10(11):935-41. DOI:10.1038/nsb974 |
- Martínez-Fleites C, Ortíz-Lombardía M, Pons T, Tarbouriech N, Taylor EJ, Arrieta JG, Hernández L, and Davies GJ. (2005). Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus. Biochem J. 2005;390(Pt 1):19-27. DOI:10.1042/BJ20050324 |
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Sinnott, M.L. (1990) Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90, 1171-1202. DOI: 10.1021/cr00105a006