Glycoside Hydrolase Family 12

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• Author: ^^^Mats Sandgren^^^
• Responsible Curator: ^^^Mats Sandgren^^^

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Glycoside Hydrolase Family GHnn
Clan	GH-C
Mechanism	retaining
Active site residues	known
CAZy DB link
http://www.cazy.org/fam/GH12.html

Substrate specificities

The substrate specificities found among the glycoside hydrolases of family 12 are: endo-β-1,4-glucanase (EC 3.2.1.4), xyloglucan hydrolase (EC 3.2.1.151), endo-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan endo-transglycosylase (EC 2.4.1.207).

Kinetics and Mechanism

GH Family 12 enzymes are retaining enzymes, as first shown by NMR studdies [1] on endoglucanase 3 from Humicola insolens, and is believed to follow a classical Koshland double-displacement mechanism in which a glycosyl-enzyme intermediate is formed and subsequently this intermediate is hydrolysed via oxocarbenium-ion transition states. No detailed studies involving both steady state and pre-steady state kinetic have yet been reported for GH family 12.

Catalytic Residues

The catalytic nucleophile and the general acid/base catalyst of GH family 12 enzymes was initialy predicted by sequence homology to the xylanase members of GH family 11, a glycoside hydrolase family where the catalytic nucleophile was first identified in the Bacillus circulans endo-xylanase through trapping of the 2-deoxy-2-fluoroxylobiosyl-enzyme intermediate and subsequent peptide mapping via LC-MS/MS technologies [2]. GH family 11 and 12 together form clan C of related GH families. The prediction of the catalytic nucleophile and the general acid/base of GH family 12 enzymes was later confirmed to be right whe the first three dimensional structure of a GH family 12 enzyme was determined, that of Streptomyces Lividans CelB [3]. The catalytic nucleophile was subsequentialy confirmed in Streptomyces Lividans CelB to be Glu 120 by using the same labeling strategy used for detecting the catalytic nucleophile of GH family 11 [4].

Three-dimensional structures

Content is to be added here.

Family Firsts

First sterochemistry determination

Humicola insolens endoglucanase 3 by NMR [1].

First catalytic nucleophile identification

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First general acid/base residue identification

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First 3-D structure

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References

1. Schou C, Rasmussen G, Kaltoft MB, Henrissat B, and Schülein M. (1993). Stereochemistry, specificity and kinetics of the hydrolysis of reduced cellodextrins by nine cellulases. Eur J Biochem. 1993;217(3):947-53. DOI:10.1111/j.1432-1033.1993.tb18325.x |
2. Miao S, Ziser L, Aebersold R, and Withers SG. (1994). Identification of glutamic acid 78 as the active site nucleophile in Bacillus subtilis xylanase using electrospray tandem mass spectrometry. Biochemistry. 1994;33(23):7027-32. DOI:10.1021/bi00189a002 |
2. Sulzenbacher G, Shareck F, Morosoli R, Dupont C, and Davies GJ. (1997). The Streptomyces lividans family 12 endoglucanase: construction of the catalytic cre, expression, and X-ray structure at 1.75 A resolution. Biochemistry. 1997;36(51):16032-9. DOI:10.1021/bi972407v |
4. Zechel DL, He S, Dupont C, and Withers SG. (1998). Identification of Glu-120 as the catalytic nucleophile in Streptomyces lividans endoglucanase celB. Biochem J. 1998;336 ( Pt 1)(Pt 1):139-45. DOI:10.1042/bj3360139 |