Glycoside Hydrolase Family 72

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• Authors: ^^^Ramon Hurtado-Guerrero^^^ and ^^^Thierry Fontaine^^^
• Responsible Curator: ^^^Bernard Henrissat^^^

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Glycoside Hydrolase Family GH72
Clan	none, (βα)8 fold
Mechanism	retaining
Active site residues	known
CAZy DB link
https://www.cazy.org/GH72.html

Substrate specificities

The GH72 family is formed exclusively by transglycosylases of the fungal kindgom whose activity was firstly characterized in Aspergillus fumigatus

The GH72 family is formed exclusively by transglycosylases of the fungal kindgom whose activity was firstly characterized in Aspergillus fumigatus [1] and yeasts [2, 3, 4]. These GPI-anchored plasma membrane enzymes elongate and remodel the β-1,3 glucan of the cell wall [4, 5, 6, 7, 8, 9]. This activity is due to their catalytic domain is located in the external part of the plasma membrane. Two sub-families have been described for GH72 family members depending on the presence or absence of a C-terminal cysteine rich domain (carbohydrate binding domain, CBM43) in addition to the catalytic domain [10]. This is an example of how to make references to a journal article [11]. (See the References section below). Multiple references can go in the same place like this [11, 12]. You can even cite books using just the ISBN [13]. References that are not in PubMed can be typed in by hand [14].

Kinetics and Mechanism

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Catalytic Residues

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Three-dimensional structures

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Family Firsts

First stereochemistry determination

Cite some reference here, with a short (1-2 sentence) explanation [11].

First catalytic nucleophile identification

Cite some reference here, with a short (1-2 sentence) explanation [14].

First general acid/base residue identification

Cite some reference here, with a short (1-2 sentence) explanation [12].

First 3-D structure

Cite some reference here, with a short (1-2 sentence) explanation [13].

References

11. Comfort DA, Bobrov KS, Ivanen DR, Shabalin KA, Harris JM, Kulminskaya AA, Brumer H, and Kelly RM. (2007). Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Biochemistry. 2007;46(11):3319-30. DOI:10.1021/bi061521n |
12. He S and Withers SG. (1997). Assignment of sweet almond beta-glucosidase as a family 1 glycosidase and identification of its active site nucleophile. J Biol Chem. 1997;272(40):24864-7. DOI:10.1074/jbc.272.40.24864 |

14. Sinnott, M.L. (1990) Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90, 1171-1202. DOI: 10.1021/cr00105a006